대한의생명과학회지 (Biomedical Science Letters)

  • 제15권3호
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  • Pages.217-227
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  • 2009
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지

The Effect of Pomegranate Extracts on the Menopausal Syndromes

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  • 이혜영 (연세대학교 보건과학대학)
  • Kim, Hyun-Chul (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Kum, Eun-Joo (Hanilyanghang Co. Ltd.) ;
  • Kwon, Do-Hyoung (Hanilyanghang Co. Ltd.) ;
  • Lee, Hye-Young (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
  • 발행 : 2009.09.30
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초록

The present study was set to evaluate the effect of pomegranate extracts on improvement of the menopausal syndromes such as face flushing in ovariectomized rats by carrying out short- and long-term experiments. Pomegranate extracts used to feed rats were prepared from the pulp part which does not contain the rid of the pomegranate, and were dissolved in propylene glycol. From the short-term (16 days) experiment, it was clear that when the 25, 250, 1,250 mg/kg/day concentrations of pomegranate extracts were orally fed to ovariectomized rats, the body temperature of the rats in all the 3 groups were decreased with statistical significance compared to other control groups which were fed with propylene glycol only. Especially, the body temperature decreased by $2.7^{\circ}C$ compared to control groups even when the pomegranate extracts were fed at the low concentration of 25 mg/kg/day implying the usefulness of pomegranate extracts in improving face flushing troubles. In addition, the body weight of the groups fed with pomegranate extracts also decreased when compared to groups fed with only propylene glycol, and the results were also statistically significant. In case of the estradiol level in the blood of rats, the levels were somewhat higher in the groups fed with pomegranate extracts than the control groups, even though the difference was not statistically significant. As found from the results of the short-term experiment, in long-term experiment, the groups fed with pomegranate extracts showed statistically significant decrease in the body temperature and the body weight, whereas the increase of the estradiol levels in blood in each groups were statistically insignificant. During the short- and long-term experiments, no sign of toxicity was found in rats fed with pomegranate extracts indicating no toxic side effects of the pomegranate extracts when orally fed. The concentrations of pomegranate extracts 25, 250, 1250 mg/kg/day treated to ovariectomized rats in this study can be estimated to be 1.5, 15, and 75 g/day when treated to women whose body weight is 60 kg which is average for women with menopausal syndromes. Since even the 75 g/day of high concentration of pomegranate extracts did not show any toxicity in short- and long-term experiments, taking 1.5 g/day concentration of pomegranate extracts would be safe dose for not causing any side effects. Therefore, it can be concluded from the results of this study that taking 1.5 g/day of pomegranate extracts for certain period time will improve the menopausal syndromes including face flushing.

키워드

참고문헌

  1. Abe T, Chow JWM, Lean JM. Chamber TJ. Estrogen does not restore bone lost after ovariectomy in the rat. J Bone Miner Res. 1993. 8: 831-838. https://doi.org/10.1002/jbmr.5650080709
  2. Aitken JM, Armstrong E, Anderson JB. Osteoporosis after oophorectomy in the mature female rat and the effect of oestrogen and-or progestogen replacement therapy in its prevention. J Endocrinol. 1972. 55: 79-87. https://doi.org/10.1677/joe.0.0550079
  3. Albertazzi, Pansini F, Bottanzzi M, Bonaccorsi G, De AD, Morton MS. Dietary soy supplementation and phytoestrogen levels. Obstetrics Gynecol. 1999. 94: 229-231. https://doi.org/10.1016/S0029-7844(99)00275-6
  4. Aviram M, Dornfeld L, Rosenblat M, Volkova N, Kaplan M, Coleman R, Hayek T, Presser D, Fuhrman B. Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. Am J Clin Natr. 2000. 71: 1062-1076. https://doi.org/10.1093/ajcn/71.5.1062
  5. Cauley JA, Zmuda JM, Ensrud KE, Bauer DC. Timing of estrogen replacement therapy for optimal osteoporosis prevention. J Clin Endocrinol Metab. 2001. 86: 5700-5705. https://doi.org/10.1210/jc.86.12.5700
  6. Chattoraj SC. Endocrine function in fundamentals of clinical chemistry. NW Tietz eds WB Saunders Chap. 1976. 13: 699-823.
  7. Clavel-Chapelon F. Hormone replacement therapy and risk of breast cancer. Press Med. 2000. 21: 1688-1693.
  8. Coldham NG, Sauer MJ. Identification, quantitation and biological activity of phytoestrogens in dietary supplement for breast enhancement. Food Chem Toxicol. 2001. 39: 1211-1224. https://doi.org/10.1016/S0278-6915(01)00081-3
  9. Johnson EB, Muto MG, Yanushpolsky EH, Mutter GL. Phytoestrogen supplementation and endometrial cancer. Obstetrics Gynecol. 2001. 98: 947-950. https://doi.org/10.1016/S0029-7844(01)01542-3
  10. Kim JS, Cho JC, Choi CO, Kim WH. Studies on climacteric symptoms and serum concentrations of LH, FSH, and $Estradiol-17{\beta}$ in early postmenopausal woman. The Keimyung univ Med J. 1985. 4: 248-253.
  11. Kim SH, Kim IH, Kang BH, Cha TY, Lee JH, Rim SO, Song KS, Song BH, Kim JG, Lee JM. Analysis of extraction characteristics of phytoestrogen components from punica granatum L. J Korean Soc Appl Biol Chem. 2005. 48: 352-357.
  12. Kim JS, Park JH, Cho HS, Park JS, Hong EK. Effect of plant extract (FGF271) on estrogen replacement. Korean J Biotechnol Bioeng. 2002. 17: 409-415.
  13. Koh, JH, Hwang MO, Moon JS, Hwang SY, Son JY. Antioxidative and antimicrobial activities of pomegranate seed extracts. Korean J Food Cookery Sci. 2005. 21: 171-179.
  14. Kurachi H, Murata Y. Clinical feat ures affecting the results of estrogen replacement therapy on bone density in Japanese postmenopausal women. Gynecol Obstet Invest. 2001. 52: 223-226. https://doi.org/10.1159/000052979
  15. Lee JY. Hormone replacement therapy in postmenopausal women. 1993. J Klima. 12: 27-36.
  16. Lee YH, Hyun SH, Choung SY. Effect of singled and mixed pomegranate on postmenopausal symptoms in ovariectomized rats. Yakhak Hoeji. 2006. 50: 177-183.
  17. Morishige K, Matsumoto K, Ohmichi M, Nishio Y, Adachi K, Hayakawa J, Nukui K, Tasaka K, Kurachi H, Murata Y. Clinical features affecting the results of estrogen replacement therapy on bone density in Japanese postmenopausal women. Gynecol Obstet Invest. 2001. 52: 223-226. https://doi.org/10.1159/000052979
  18. Lemay A, Dodin S, Kadri N, Jacques H, Forest JC. Flaxseed dietary supplement versus hormone replacement therapy in hypercholesterolemic menopausal women. Obstetrics Gynecol. 2002. 100: 495-504. https://doi.org/10.1016/S0029-7844(02)02123-3
  19. Life-table. 2002. Korea National Statistical Office. Dea-jeon, Korea.
  20. Longtin R. The pomegranate: nature,s power fruit-. J Natl Cancer Inst. 2003. 95: 346-348. https://doi.org/10.1093/jnci/95.5.346
  21. Moneam NMA, Sharaky ASE, Badreldin MM. Oestrogen content of pomegranate seeds. J Chromatography 1988. 438: 438-442. https://doi.org/10.1016/S0021-9673(00)90278-4
  22. Nugteren DH, Christ-Hazelhof E. Naturally occurring conjugated octadecatrienoic acid are strong inhibitors of prostaglandin biosynthesis. Prostaglandins 1987. 33: 403-417. https://doi.org/10.1016/0090-6980(87)90022-0
  23. Okada M, Hayashi N, Kometani M, Nakao K, Inukai T. Influences of ovariectomy and continuous replacement of ${\beta}-estradiol$ on the tail skin temperature and behavior in the forced swimming test in rats. Jpn J Pharmacol. 1997. 73: 93-96. https://doi.org/10.1254/jjp.73.93
  24. Okamoto JM, Yoko OH, Hideyuki Y, Hiroyuki Y. Pomegrante extract improves a depressive state and bone properties in menopausal syndrome model ovariectomized mice. J Ethnopharmacol. 2004. 92: 93-101. https://doi.org/10.1016/j.jep.2004.02.006
  25. Patel C, Dadhaniya P, Hingorani L, Soni MG. Safety assessment of pomegranate fruit extrac t: Acute and subchronic toxicity studies. Food Chem Toxicol. 2008. 46: 2728-2735. https://doi.org/10.1016/j.fct.2008.04.035
  26. PH van de Weijer, Barentsen R. Isoflavones from red clover significantly reduce menopausal hot flush symptoms compared with placebo. Maturitas. 2002. 42: 187-193. https://doi.org/10.1016/S0378-5122(02)00080-4
  27. Poyranzogle E, Gokmen V, Artjk N. Organic acids and phenolic compounds in pomegranates Grown in turkey. J Food composition analysis 2002. 15: 567-575. https://doi.org/10.1016/S0889-1575(02)91071-9
  28. Ross RK, Annlia PH, Wan PC, Pike MC. Effect of hormone replacement therapy on breast cancer risk: estrogens versus estrogen plus progestin. J Natl Cancer Institute 2000. 92: 328-332. https://doi.org/10.1093/jnci/92.4.328
  29. Shim SM, Choi SW, Bae SJ. Effect of Punica granatum L. fractions on quinone reductase induction and growth inhibition on several cancer cells. J Korean Soc Food Sci Nutr. 2001. 30: 80-85.
  30. Song BH, Tran NA, Bae SY. Pomegranate as resource of phytoestrogen and anticancer substances. Kor J Microbiol Biotechnol. 2007. 35: 81-97.
  31. Vidal A, Fallarero A, Pena BR, Medina ME, Gra B, Rivera F, Gutierrez Y, Vuorela PM. Studies on the toxicity of Punica granatum L. (Punicaceae) whole fruit extracts. J Ethnopharmacol. 2003. 89: 295-300. https://doi.org/10.1016/j.jep.2003.09.001
  32. Wronski TJ, Cintron M, Dann LM. Temporal relationship between bone loss and increased bone turnover in ovariectomized rats. Calcif Tissue Int. 1988. 43: 179. https://doi.org/10.1007/BF02571317