Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Rubus coreanus Extract Attenuates Acetaminophen Induced Hepatotoxicity; Involvement of Cytochrome P450 3A4

  • Lee, Young-Ik (Liver Cell Signal Transduction Lab., Metabolic Syndrome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Whang, Kyung-Eun (Liver Cell Signal Transduction Lab., Metabolic Syndrome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Jin-Sook (Lee's Biotech Co., BVC#203, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Byung-Min (Division of Hepatology and Gastroenterology, Bupyong Serim Genenral Hospital) ;
  • Lee, Sang-Bum (School of Life Sciences and Biotechnology, Korea University) ;
  • Dong, Mi-Sook (School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Tae-Hyun (Department of Gastroenterology, Wonkwang University, School of Medicine and Hospital)
  • Published : 2009.10.31
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Abstract

Foods of plant origin, especially fruits and vegetables, have attracted attention because of their potential benefits to human health. In this report, Rubi Fructus (RF), the dried unripe fruit of Rubus coreanus Miq (Rosaceae) and ellagic acid (EA) purified from RF were used to test their potential hepatoprotective effect against acetaminophen (AAP)-induced hepatotoxicity in rats. RF extract (RFext) and EA reduced the elevated levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) in serum and the content of lipid peroxide in liver by AAP administration, while the increment of the cellular glutathione (GSH) content and the induction of glutathione S-transferase (GST) and glutathione peroxidase (GSH-PX) which were decreased by AAP administration. RFext and EA from RFext did not affect the two major form of cytochrome P450s, cytochrome P450 2E1 (CYP2E1) and cytochrome P450 1A2 (CYP1A2), but downregulated the cytochrome P450 3A4 (CYP3A4) related to the conversion of AAP to N-acetyl-P-benzoquinone imine (NAPQI). These results suggest that RFext and EA from RF exhibit a hepatoprotective effect not only by increasing antioxidant activities but also by down-regulating CYP3A4 in the AAP-intoxicated rat.

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References

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