Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimutagenic and Antitumor Effects of Codonopsis lanceolata Extracts

더덕 추출물의 항돌연변이 및 항종양 효과

  • Kim, Soo-Hyun (Dept. of Food Science and Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Choi, Hyun-Jin (Dept. of Food Science and Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Chung, Mi-Ja (The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University) ;
  • Cui, Cheng-Bi (Dept. of Food Science and Engineering, Agricultural College of Yanbian University) ;
  • Ham, Seung-Shi (Dept. of Food Science and Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University)
  • 김수현 (강원대학교 생명공학부) ;
  • 최현진 (강원대학교 생명공학부) ;
  • 정미자 (강원대학교 BK21 사업단(뉴트라슈티컬 바이오)) ;
  • 최승필 (연변대학농학원 식품과학부) ;
  • 함승시 (강원대학교 생명공학부)
  • Published : 2009.10.31
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Abstract

This study was carried out to investigate the mutagenic, antimutagenic, cytotoxicity and antitumor effect of Codonopsis lanceolata (CL). CL was extracted with 70% ethanol and then further fractionated to hexane, chloroform, ethyl acetate, butanol and water. Antimutagenic, cytotoxicity and antitumor effects of CL extracts were measured by using Ames test, SRB method, and the tumor growth inhibition test. CL extracts did not show any mutagenicity in the Ames test; however, 70% ethanol extracts and its fractions had strong antimutagenic effects against mutation induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4NQO). The ethyl acetate fraction of CL (200 ${\mu}g$/plate) showed approximately 72.1% inhibitory effect on the mutagenesis induced by 4NQO against TA98 strain, whereas 69.6% and 67.0% inhibitions were observed on the mutagenesis induced by MNNG and 4NQO against TA100 strain. In anticancer effects, the cytotoxicity of CL extract and its fractions against cancer cell lines including human cervical adenocarcinoma (HeLa), human hepatocellular carcinoma (HepG2), human breast adenocarcinoma (MCF-7), human lung carcinoma (A549) and transformed primary human embryo kidney (293) were investigated. The treatment of 1 mg/mL CL ethyl acetate fraction had the highest cytotoxicity of 74.5%, 70.7% and 80.3% against HeLa, MCF-7 and A549 cells, respectively. In contrast, the extract and its fractions showed only 2$\sim$31% cytotoxicity for a normal human kidney cell line (293). In vivo anticancer effect of CL extract was tested using Balb/c mice transplanted sarcoma-180 cells. CL ethyl acetate fraction showed the highest inhibition rate of 56.4% at the 50 mg/kg concentration.

본 연구는 더덕의 돌연변이원성, 항돌연변이원성, 세포독성, 항종양 효과를 조사하기 위해서 수행되었다. 더덕을 70% 에탄올로 추출하여 추출용매에 따라 핵산, 클로로포름, 에틸아세테이트, 부탄올과 물 층으로 분획하였다. Ames test assay, SRB assay와 sarcoma-180 세포를 이용한 항종양 실험을 실시하였다. Ames test 결과, 더덕 에탄올 추출물은 돌연변이원성을 나타내지 않았다. 더덕 에틸아세테이트분획물은(200 ${\mu}g$/plate) 4NQO에 대하여 S. Typhimurium TA98과 TA100에서 각각 72.1% 및 67.0%의 억제율을 나타내었으며, MNNG에 대한 S. Typhimurium TA100은 69.6%의 억제율을 나타내었다. 더덕 추출물 및 분획물의 암세포성장 억제효과를 살펴보기 위해 인간 자궁경부암세포(HeLa), 인간 간암세포(HepG2), 인간 유방암세포(MCF-7), 인간 폐암세포(A549) 및 인간 신장정상세포(293)를 사용하였다. 더덕 에틸아세테이트 분획물을 1 mg/mL의 농도로 처리하였을 때 각각 74.5%(HeLa), 70.7%(MCF-7) 및 80.3%(A549)의 가장 높은 억제활성을 나타내었다. 반면에 인간 정상 신장세포(293)에서는 2$\sim$31%의 세포독성을 나타내었다. In vivo에서 더덕 추출물 및 분획물의 항암 효과를 검토하기 위하여 Balb/c 마우스에 sarcoma-180 종양세포로 고형암을 유발시켰다. 그 결과 더덕 에틸아세테이트 분획물의 최고농도 50 mg/kg에서 56.4%의 고형암 성장 억제 효과를 나타내었고, 이는 다른 추출물 및 분획물 중에서 가장 높은 억제율이었다.

Keywords

References

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