KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimization of Fabrication Conditions for Immunosensor Strip to Detect Escherichia coli O157 : H7

Escherichia coli O157 : H7 탐지용 면역센서스트립 제작 조건 최적화

  • Park, So-Jung (Program of Integrated Biotechnology, Graduate School of Bio-and Information Technology, Hankyong National University) ;
  • Kim, Young-Kee (Program of Integrated Biotechnology, Graduate School of Bio-and Information Technology, Hankyong National University)
  • 박소정 (한경대학교 생물환경정보통신전문대학원 바이오융합기술) ;
  • 김영기 (한경대학교 생물환경정보통신전문대학원 바이오융합기술)
  • Published : 2009.06.29
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Abstract

In this study, the optimization of fabrication conditions was accomplished to make immuno-strip biosensor by the combination of enzyme linked immunosorbent assay (ELISA) and immuno-chromatographic strip techniques for the detection of Escherichia coli O157 : H7. Optimal fabrication conditions of capture antibody concentration, detection antibody concentration, and additive composition of running buffer solution were determined. Optimal concentration was determined as 1.0 mg/mL for both of capture antibody and detection antibody. A composition of 0.5% Tween20 and 3% BSA were selected as optimal additive for buffer solution to prevent non-specific binding.

본 연구에서는 ELISA와 면역-크로마토그래피 스트립기술을 결합하여 E. coli O157 : H7을 탐지할 수 있는 면역스트립 센서를 제작하기 위한 제작조건의 최적화 연구를 수행하였다. 포획항체 농도, 탐지항체 농도, 완충용액 첨가제 조성의 면역스트립 제작 또는 운전인자들의 최적화 조건을 결정하였다. 포획항체의 농도는 1 mg/mL를 최적 조건으로 선정하였고, 탐지항체의 최적 농도도 1 mg/mL로 결정하였다. 비특이적 결합을 방지하기 위한 시료 희석용 완충용액의 첨가제 조성으로는 0.5% Tween 20와 3% BSA 혼합 사용을 선정하였다.

Keywords

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