대한의용생체공학회:의공학회지 (Journal of Biomedical Engineering Research)

  • 제30권4호
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  • Pages.347-354
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  • 2009
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  • 1229-0807(pISSN)
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  • 2288-9396(eISSN)
대한의용생체공학회 (The Korean Society of Medical and Biological Engineering)
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전기자극펄스에 대한 변성망막 신경절세포의 응답특성 분석

Analysis of Neuronal Activities of Retinal Ganglion Cells of Degenerated Retina Evoked by Electrical Pulse Stimulation

  • 류상백 (연세대학교 보건과학대학 의공학과) ;
  • 이종승 (연세대학교 보건과학대학 의공학과) ;
  • 예장희 (충북대학교 의과대학 생리학교실) ;
  • 구용숙 (충북대학교 의과대학 생리학교실) ;
  • 김지현 (연세대학교 보건과학대학 의공학과) ;
  • 김경환 (연세대학교 보건과학대학 의공학과)
  • Ryu, Sang-Baek (Department of Biomedical Engineering, College of Health Science, Yonsei University) ;
  • Lee, Jong-Seung (Department of Biomedical Engineering, College of Health Science, Yonsei University) ;
  • Ye, Jang-Hee (Department of Physiology, Chungbuk National University Medical School) ;
  • Goo, Yong-Sook (Department of Physiology, Chungbuk National University Medical School) ;
  • Kim, Chi-Hyun (Department of Biomedical Engineering, College of Health Science, Yonsei University) ;
  • Kim, Kyung-Hwan (Department of Biomedical Engineering, College of Health Science, Yonsei University)
  • 발행 : 2009.08.30
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초록

For the reliable transmission of meaningful visual information using prosthetic electrical stimulation, it is required to develop an effective stimulation strategy for the generation of electrical pulse trains based on input visual information. The characteristics of neuronal activities of retinal ganglion cells (RGCs) evoked by electrical stimulation should be understood for this purpose. In this study, for the development of an optimal stimulation strategy for visual prosthesis, we analyzed the neuronal responses of RGCs in rd1 mouse, photoreceptor-degenerated retina of animal model of retinal diseases (retinitis pigmentosa). Based on the in-vitro model of epiretinal prosthesis which consists of planar multielectrode array (MEA) and retinal patch, we recorded and analyzed multiunit RGC activities evoked by amplitude-modulated electrical pulse trains. Two modes of responses were observed. Short-latency responses occurring at 3 ms after the stimulation were estimated to be from direct stimulation of RGCs. Long-latency responses were also observed mainly at 2 - 100 ms after stimulation and showed rhythmic firing with same frequency as the oscillatory background field potential. The long-latency responses could be modulated by pulse amplitude and duration. From the results, we expect that optimal stimulation conditions such as pulse amplitude and pulse duration can be determined for the successful transmission of visual information by electrical stimulation.

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참고문헌

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