Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Role of Two New Phytotoxins in the Pathogenicity of Botrytis cinerea

두 개의 새로운 phytotoxin의 Botrytis cinerea 병원성에시의 역할

  • Kim, Geum-Jung (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Yoon, Mi-Young (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Kim, Heung-Tae (Department of Plant Medicine, Chungbuk National University) ;
  • Choi, Gyung-Ja (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung-Soo (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong-Ho (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Park, Myung-Soo (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University) ;
  • Kim, Jin-Cheol (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology)
  • 김금정 (한국화학연구원 산업바이오화학연구센터) ;
  • 윤미영 (한국화학연구원 산업바이오화학연구센터) ;
  • 김흥태 (충북대학교 식물의학과) ;
  • 최경자 (한국화학연구원 산업바이오화학연구센터) ;
  • 장경수 (한국화학연구원 산업바이오화학연구센터) ;
  • 최용호 (한국화학연구원 산업바이오화학연구센터) ;
  • 박명수 (한국화학연구원 산업바이오화학연구센터) ;
  • 차병진 (충북대학교 식물의학과) ;
  • 김진철 (한국화학연구원 산업바이오화학연구센터)
  • Published : 2009.08.01
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Abstract

In the course of study on the roles of phytotoxins in the pathogenicity of Botrytis cinerea, we isolated two novel phytotoxins. They were identified as 3-O-acetyl botcinol and 3-O-acetyl botcinolide. In this study, we investigated correlation between the two phytotoxins and the pathogenicity of B. cinerea. In liquid cultures, the two phytotoxins were not produced by three low pathogenic isolates out of 25 B. cinerea isolates. Among strong or moderate pathogenic isolates, some produced the two phytotoxins, but the others did not. On the other hand, the ethyl acetate extracts of fermentation broths of 10 out of 25 isolates showed phytotoxic activity against various plants tested in a whole plant assay. The phytotoxins were detected in all of the 10 phytotoxic ethyl acetate extracts. In planta, the two phytotoxins were detected in all of the plant tissues infected with strong pathogenic isolates. However, there was no correlation between the ability of B. cinerea isolates to produce the two phytotoxins and their pathogenicities. The two phytotoxins began to detect in tomato plant tissues infected with B. cinerea 2-16 at 3 days after inoculation, increased gradually till 4 days after inoculation, and then decreased. The above results suggest that 3-O-acetyl botcinol and 3-O-acetyl botcinolide are one of pathogenicity factors for B. cinerea, but not a primary determinant of its pathogenicity.

Botrytis cinerea 균주의 병원성에 있어서 phytotoxin의 역할을 규명하는 연구를 하던 중에 B. cinerea 2-16균주가 생산하는 새로운 phytotoxin을 분리 동정하였다. 두 개의 물질은 3-O-acetyl botcinol과 3-O-acetyl botcinolide로 동정되었다. 본 연구는 두 개의 물질과 B. cinerea의 병원성과의 상관관계를 규명하고자 실시하였다. 25개의 B. cinerea 균주 중에서 병원성이 약한 3개의 B. cinerea 균주는 액체배지에서 두 phytotoxin을 생산하지 않았다. 병원성이 강하거나 중간정도인 균주들은 두 phytotoxin을 생산하는 균과 생산하지 않는 균으로 나뉘었다. 한편, 25개의 균주 중에서 10균주의 액체배양액 에틸아세테이트 추출물이 9개의 식물체를 이용한 whole plant assay에서 phytotoxicity를 나타냈으며, 이들 10개 추출물 모두에서 두 phytotoxin이 검출되었다. In planta 실험에서는 병원성이 강한 균주에 감염된 식물조직에서 모두 두 phytotoxin이 검출되었다. 하지만 두 phtotoxin의 생산량과 병원성과는 상관관계를 보이지 않았다. 두 phytotoxin은 B. cinerea 2-16 균주에 감염된 토마토 조직에서 3일째부터 검출되기 시작해 4일째는 가장 크게 증가하였으며 그 후 감소하였다. 이상의 실험 결과 3-O-acetyl botcinol과 3-O-acetyl botcinolide는 B. ciuereo의 발병과정에 있어서 중요한 역할을 할 것으로 추정되지만 병을 일으키는데 일차적인 요인은 아닐 것으로 사료된다.

Keywords

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