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Control of Bakanae Disease of Rice by Seed Soaking into the Mixed Solution of Procholraz and Fludioxnil

Prochlornz와 fludioxonil 혼용침지소독에 의한 벼 키다리병 방제

  • Park, Woo-Sik (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Choi, Hyo-Won (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Han, Seong-Suk (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Shin, Dong-Beum (Crop Environment Research Division, National Institute of Crop Science, RDA) ;
  • Shim, Hyeong-Kwon (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Jung, En-Seon (Variety Testing Division, Korea Seed & Variety Service) ;
  • Lee, Se-Weon (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Lim, Chun-Keun (Division of Bio-Resources Technology, College of Agriculture and Life Science, Kangwon National University) ;
  • Lee, Yong-Hwan (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
  • 박우식 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 최효원 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 한성숙 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 신동범 (국립식량과학원 작물환경과) ;
  • 심형권 (국립식량과학원 바이오에너지작물센터) ;
  • 정은선 (국립종자원 재배시험과) ;
  • 이세원 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 임춘근 (강원대학교 농업생명과학대학 응용생물공학과) ;
  • 이용환 (농촌진흥청 국립농업과학원 농업미생물과)
  • Published : 2009.08.01

Abstract

These experiments were conducted to improve the effect of seed disinfection on rice seed severely infected Bakanae disease by seed soaking into mixed solution of prochloraz EC and fludioxonil FS. We investigated the effects of various concentrations of two fungicides mixed solution on spore germination and mycelial growth of Fusarium fujikuroi. Mycelial growth was inhibited 100% at $10{\mu}g$/ml of prochloraz and 33.3% at $80{\mu}g$/ml of fludioxonil. Spore germination was inhibited 81.4% at $40{\mu}g$/ml of prochloraz. Interestingly, mixed solution of $5{\mu}g$/ml or $10{\mu}g$/ml of each fungicide inhibitied 100% of mycelial growoth and 99.2% of spore germination, respectively. Severely infected rice seeds soaked into mixed solution composed of $125{\mu}l$/ml of prochloraz and $50{\mu}l$/ml of fludioxonil showed 2.1% of disease symptoms compared to 20.4% of prochloraz $125{\mu}l$/ml, but higher concentrations of prochloraz decreased the seedling stand rate. When the seed soaking time was longer and temperature was higher, control effect on Bakanae disease was improved, but seedling stand was lower about 80% over $35^{\circ}C$.

본 연구에서는 키다리병에 심하게 감염된 종자를 prochlornz EC와 fludioxonil FS을 혼용하여 종자소독 효과를 증진시키기 위해 혼용처리 시 병원균 균사 및 포자 억제효과를 분석하고 혼용조건을 설정 하였다. 균사생장 억제 검정에서는 단제 사용 시 prochloraz는 $10{\mu}g$/ml 처리에서 100% 억제한 반면 fludioxonil은 $80{\mu}g$/ml 처리에서 33.3%의 억제율을 나타내었고, 두 가지 살균제의 혼용 시 각각 $5{\mu}g$/ml씩 혼용처리에서 100% 억제하였다. 포자발아 억제 검정의 경우 prochloraz $40{\mu}g$/ml 단독 처리에서 81.4% 발아를 억제하였지만 prochloraz와 fludioxonil 각각 $10{\mu}g$/ml 혼용처리에서는 99.2%의 억제율을 보였다. 감염종자를 대상으로 혼용침지소독 시 prochloraz $125{\mu}l$/ml와 fludioxonil $50{\mu}l$/ml 혼용 침지할 경우 발병율이 2.1%로 높은 방제 효과를 보이고 입모율에는 영향을 미치지 않았지만 prochloraz 농도가 높아질수록 입모율이 낮아지는 경향이 있었다. 침지시간이 길고 온도가 높을수록 키다리병 방제효과가 증진된 반면 $35^{\circ}C$ 이상에서는 입모율이 80% 내외로 낮아지는 경향을 보였는데 관행방법인 prochloraz $125{\mu}l$/ml 단독으로 $30^{\circ}C$에서 48시간침지 시 57.6%의 방제가를 나타낸 반면 prochloraz $125{\mu}l$/ml와 fludioxonil $50{\mu}l$/ml를 혼용하여 $30^{\circ}C$에서 48시간 첨지할 경우 방제가 96.7%, 입모율 88.0%로 키다리병 방제에 효과적이면서 식물체에 안정적인 방제효과를 나타내었다.

Keywords

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