The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory Effects of Herbal Formula KCNS-001 for Mitigating Atopic Dermatitis

한약조성물 KCNS-001이 자유라디칼과 염증매개인자에 미치는 영향

  • Lee, Jeong-Bok (Sonimedi Inc) ;
  • Choi, Jae-Hwan (Orieltal Herb Cosmeceutical R&D Center, LG Household & Healthcare Ltd/Research Park) ;
  • Bang, Ok-Sun (Brain Disease Research Center, Korea Institute of Oriental Medicine) ;
  • Yu, Young-Beob (Brain Disease Research Center, Korea Institute of Oriental Medicine)
  • 이정복 ((주) 소니메디) ;
  • 최재환 ((주) LG생활건강 기술연구원 후 한방피부과학연구소) ;
  • 방옥선 (한국한의학연구원 뇌질환연구센터) ;
  • 유영법 (한국한의학연구원 뇌질환연구센터)
  • Published : 2009.09.30
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Abstract

Objectives : We determined the anti-inflammatory activity of KCNS-001 that is a herbal formula including 6 medicinal plants and that are used to mitigate atopic dermatitis in oriental medicine. Methods : To evaluate anti-inflammatory effect of KCNS-001, we measured the production of reactive oxygen species (ROS), nitric oxide (NO) and cyclooxygenase-2 (COX-2) in LPS-activated Raw 264.7 cells. Cell viability was determined by MTT assay. The concentrations of ROS and relative level of NO were measured with DPPH assay and Griess reagent, respectively. COX-2 and TNF-$\alpha$ were detected by enzyme immuno assay (EIA) and enzyme-linked immunosorbent assay (ELISA). Results : ROS and NO production were reduced by KCNS-001 in a dose-dependent manner. KCNS-001 significantly inhibited activity of COX-2 and suppressed the release of tumor necrosis factor-alpha (TNF-$\alpha$). Conclusions : These results indicate that the KCNS-001 may have an anti-inflammatory agent for the treatment of various inflammatory disease.

Keywords

References

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