The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-oxidative and Anti-inflammatory Effect of Fractionated Extracts of Smilacis Glabrae Rhizoma in Human Umbilical Vein Endothelial Cell

혈관내피세포에서 토복령(土茯苓)의 항산화 및 항염증 효과

  • Lee, Chang-Hyun (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Yi, Hyo-Seung (Cardiovascular Medical Research Center, Dongguk University) ;
  • Kim, Jae-Eun (Cardiovascular Medical Research Center, Dongguk University) ;
  • Heo, Sook-Kyoung (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Cha, Chang-Min (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Won, Chan-Wook (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Park, Sun-Dong (Cardiovascular Medical Research Center, Dongguk University)
  • 이창현 (동국대학교 한의과대학 방제학교실) ;
  • 이효승 (심혈관계질환 천연물 연구개발센터) ;
  • 김재은 (심혈관계질환 천연물 연구개발센터) ;
  • 허숙경 (동국대학교 한의과대학 방제학교실) ;
  • 차창민 (동국대학교 한의과대학 방제학교실) ;
  • 원찬욱 (동국대학교 한의과대학 방제학교실) ;
  • 박선동 (심혈관계질환 천연물 연구개발센터)
  • Published : 2009.09.30
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Abstract

Objectives : Smilacis glabrae rhizoma (SG) has been traditionally used as a herbal medication of musculoskeletal disorders like arthritis, pain, convulsions, and syphilis in traditional Korean medicine. This study was investigated anti-oxidative and anti-inflammatory effect of fractionated extracts of Smilacis Glabrae Rhizoma in Human Umbilical Vein Endothelial Cell (HUVEC). Methods : SG extract prepared with methanol, and then fractionated with hexane, dichloromethane, ethylacetate, n-butanol and water. Inhibitory effect of SG onto free radical generation was determined by measuring DPPH, superoxide anions and nitric oxide scavenging activities in vitro. Cytotoxic activity of extracts on RAW 264.7 cells was measured using 5-(3-caroboxymethoxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. Intracelluar oxidation was analysed by DCF-DA assay. The nitric oxide (NO) production was measured by Griess reagent system. The levels of ICAM-1 and VCAM-1 expression were confirmed by western blot. And proinflammatory cytokines were measured by ELISA kit. Results : Our results indicated that fractionated extracts, especially ethyl acetate (EA) extract, significantly inhibited free radical generation, the TNF-$\alpha$-induced intracellular oxidation. Furthermore, the EA extract protected TNF-$\alpha$-induced adhesion to THP-1, expression of adhesion molecules accompanied by an attenuation of IL-6 and IL-8 formation in HUVEC. Conclusions : These results indicate that EA extract of SG have potential as an agent of atherosclerosis and other chronic inflammatory diseases including diabetes, hypertension, and arthritis.

Keywords

References

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