Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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The Effect of Particulate Matter 10 from Asian Dust on the Production of Reactive Oxygen Species, TGF-β, NF-κB, PDGF-α and Fibronectin in MRC-5 Fibroblast Cells

폐 섬유모세포에서 황사의 미세먼지(Particulate Matter 10)가 활성산소족과 TGF-β, NF-κB, PDGF-α, Fibronectin의 생성에 미치는 영향

  • Kim, Ah Hyun (Gachon University of Medicine and Science) ;
  • Chon, Suyeon (Division of Pulmonology, Department of Internal Medicine, Gachon University Gil Hospital) ;
  • Yoon, Jin Young (Division of Pulmonology, Department of Internal Medicine, Gachon University Gil Hospital) ;
  • Kim, Yu Jin (Division of Pulmonology, Department of Internal Medicine, Gachon University Gil Hospital) ;
  • Kyung, Sun Young (Division of Pulmonology, Department of Internal Medicine, Gachon University Gil Hospital) ;
  • Lee, Sang Pyo (Division of Pulmonology, Department of Internal Medicine, Gachon University Gil Hospital) ;
  • Park, Jeong Woong (Division of Pulmonology, Department of Internal Medicine, Gachon University Gil Hospital) ;
  • Jeong, Sung Hwan (Division of Pulmonology, Department of Internal Medicine, Gachon University Gil Hospital)
  • 김아현 (가천의과학대학교 의학전문대학원 의학과) ;
  • 전수연 (가천의과학대학교 길병원 호흡기내과) ;
  • 윤진영 (가천의과학대학교 길병원 호흡기내과) ;
  • 김유진 (가천의과학대학교 길병원 호흡기내과) ;
  • 경선영 (가천의과학대학교 길병원 호흡기내과) ;
  • 이상표 (가천의과학대학교 길병원 호흡기내과) ;
  • 박정웅 (가천의과학대학교 길병원 호흡기내과) ;
  • 정성환 (가천의과학대학교 길병원 호흡기내과)
  • Received : 2009.09.14
  • Accepted : 2009.11.04
  • Published : 2009.12.30
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Abstract

Background: Dust clouds blown by the wind from the arid deserts of Mongolia and Northeast China are known as Asian dust storms. Ambient particulate matter with a diameter <10 ${\mu}m$ ($PM_{10}$) is associated with the exacerbation of respiratory diseases and increased mortality of heart and lung disease patients. The fibrotic effects of $PM_{10}$ of Asian dust to pulmonary fibroblast cells are unknown. This study examined the production of reactive oxygen species (ROS), TGF-${\beta}$, NF-${\kappa}B$, PDGF-$\alpha$ and Fibronectin in fibroblasts exposed to Asian dust particles. Methods: Air samples were collected using a high volume air sampler (Sibata model HV500F) with an air flow of 500 L/min for at least 6 hours. The MRC-5 cells were exposed to 0, 50 and 100 ${\mu}g/mL$ of $PM_{10}$ for 24 hours. ROS was detected by measuring the level of oxidized DCF using FACS. TGF-$\beta$, NF-${\kappa}B$, PDGF-$\alpha$ and fibronectin were detected by western blotting. Results: There was no increase in the ROS, TGF-$\beta$ and PDGF-$\alpha$ levels in the MRC-5 cells exposed to $PM_{10}$. The NF-${\kappa}B$ level was higher in the MRC-5 cells exposed to 50 and 100 ${\mu}g/mL$ of $PM_{10}$ for 24 hours. The fibronectin level in the MRC-5 cells after 24 hours incubation with 50 ${\mu}g/mL$ $PM_{10}$ was significantly higher than the control group ($PM_{10}$ 50 ${\mu}g/mL$ 113.27${\pm}$8.65 of control, p=0.005). Conclusion: $PM_{10}$ from Asian dust increases the activation of NF-${\kappa}B$ and fibronectin expression in MRC-5 fibroblast cells.

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References

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