Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Sasa coreana Nakai on the Lipid Compositions of Serum in High Cholesterol Diet Rats

신의대(Sasa coreana Nakai) 추출물이 고콜레스테롤 식이 투여 흰쥐의 혈청 지질에 미치는 영향

  • Park, Jong-Ok (Department of Chemistry, Kyungsung University) ;
  • Jang, Hye-Won (Division of Endocrinology & Metabolism, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine)
  • 박종옥 (경성대학교 화학과) ;
  • 장혜원 (성균관 의대 삼성서울병원 내분비대사내과)
  • Published : 2009.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the effects of Sineui-Dae (Sasa coreana Nakai, a kind of bamboo) extracts on the improvement of serum lipid composition by using rats fed a hypercholesterol diet for 4 weeks. The experiment animals were administered with the following diets; high cholesterol diet group (HC diet) as a control and three supplemented groups with high cholesterol diets (HCW, HCM or HCH diet). Three kinds of extracts were prepared by orderly extraction with hexane, methanol, and water. We measured free cholesterol, cholesteryl ester, total cholesterol LDL- and HDL-cholesterol triglyceride and phospholipid in the serum of rats in three experimental and control groups. There were no significant differences in body weights and feed intakes between the HC control group and the extract supplemented groups. The levels of total cholesterol and LDL-cholesterol in the serum of extract supplemented groups were lower than that of the group fed with HC diet only. Serum HDL-cholesterol levet which is known as an antiatherosclerosis factor, was higher in all groups supplemented with bamboo extract by 17.2-21.9% compared to the HC control group. All groups supplemented with bamboo extracts showed the lowering effect of atherogenic index compared to the HC control group (HC group: 2.96${\pm}$0.08, HCM group: 1.48${\pm}$0.02, HCW group: 1.69${\pm}$0.04, HCH group: 1.84${\pm}$0.01). Furthermore, serum triglyceride and phospholipid decreased significantly in the HCM diet compared to the HC control diet. These results suggest that Sineui-Dae bamboo extract, especially methanol extract, has improving effects on hyperlipidemia of rats fed a high cholesterol diet.

신의대 (Sasa corean, Nakai, 대나무 일종) 추출물이 혈청지질 개선에 미치는 영향을 조사하기 위하여 1.0%의 콜레스테롤 첨가식이로 유발된 고지혈증 Sprague Dawley계 수컷 흰쥐를 이용하였다. 실험동물들은 4 종류 투여군으로 나누었으며 고콜레스테롤 투여군(HC)을 대조군으로 하였고 3 종류의 대나무추출액을 첨가한 투여군(HCW, HCM 및 HCH) 을 실험군으로 하였다. 대나무추출액은 헥산, 메탄올 및 물을 이용하여 순차적으로 추출하여 농축, 동결건조하여 사용하였다. 고콜레스테롤식이를 급여한 대조군(HC 대조군)에 비하여 대나무추출물을 첨가한 모든 투여군(HCW, HCM 및 HCH 투여군)에서 체중변화 및 식이섭취량에 미치는 영향은 별로 크지 않았다. 혈청 총 콜레스테롤 및 LDL-콜레스테롤 함량을 비교하였을 때 HC 대조군에 비하여 대나무추출물 첨가 투여군에서 18.5-23.7% 및 15.7-18.6% 감소하였으며 특히 메탄올추출물의 효과가 가장 높았다. 항동맥경화 인자로 알려진 HDL-콜레스테롤의 경우 대나무추출물 투여군에서 HC 대조군에 비하여 17.2-21.9% 정도 증가하는 것을 볼 수 있었다. 대나무추출물은 고콜레스테롤 식이를 섭취한 HC 대조군에 비하여 동맥경화지수를 낮추는 효과가 있었다(HC group : 2.96${\pm}$0.08, HCM group : 1.48${\pm}$0.02, HCW group : 1.69${\pm}$0.04, HCH group : 1.84${\pm}$0.01). 또한 혈청중의 중성지질 함량 및 인지질 함량도 메탄올추출물 투여군에서 가장 낮았다. 이러한 실험 결과들로부터 대나무 추출물, 특히 메탄올추출물이 혈청지질의 개선효과가 있으며 결과적으로 고지혈증이나 심장순환기계 질환을 예방하는 효과가 있을 것으로 사료된다.

Keywords

References

  1. Akao, Y., N. Seki, Y. Nakagawa, H. Yi, K. Matsmoto, Y. Ito, K. Ito, M. Funaoka, W. Maruyama, M. Naoi, and Y. Nozawa. 2004. A highly bioactive lignophenol derivative from bamboo lignin exhibits a potent activity to suppress aptosis induced by oxidative stress in human neuroblastoma SH-SY5Y cells. Bioorganic & Medicinal Chemistry 12, 4791-4801 https://doi.org/10.1016/j.bmc.2004.07.022
  2. Baek, J. W., S. H. Lee, and G. S. Moon, 2002. Antimicrobial activities of ethanol extracts from Korean bamboo culms and leaves. Korean J. Food Sci. Technol. 34, 1073-1078
  3. Balasubrmaniam, T., L. E. Simons, R. Chang, and J. B. Trickie. 1985. Reduction in plasma cholesterol and increase in biliary cholesterol by a diet rich in n-3 fatty acids in the rat. J. Lipid Res. 26. 284-294
  4. Beynen, C. and M. B. Katan. 1985. Why do polyunsaturated fatty acid lower serum cholesterol. Am. J. Clin. Nutr. 42. 560-570
  5. Castelli, W. P., R. J. Garrison, P. W. F. Wilson, R. D. Abott, A. Kalousdian, and W. B. Kannel. 1986. Incidence of coronary heart disease and lipoprotein cholesterol levels. JAMA. 256, 2835-2844 https://doi.org/10.1001/jama.256.20.2835
  6. Chuyen, N. V., T. Kurata, and M. Furjimaki. 1982. Antimicrobial activity of kumazasa (Sasa albro marginata). Agri. Biol. Chem. 46, 971-975 https://doi.org/10.1271/bbb1961.46.971
  7. Dwyer, J. 1995. Overview: dietary approaches for reducing cardiovascular disease risk. J. Nutr. 125, 656-665
  8. Faidley, T. Y., C. M. Luhman, T. U. Galloway, M. K. Foley, and D. C. Beitz. 1990. Effect of dietary fat source on lipoprotein composition and plasma lipid concentration in pigs. J. Nutr. 120, 1126-1138
  9. Goodman, D. S. 1964. The turnover of plasma cholesterol in man. physiol. Rev. 45, 747-760
  10. Grundy, S. M. 1987. Monounsaturated fatty acids, plasma cholesterol and coronary heart disease. Am. J. Clin. Nutr. 42, 1168-1174
  11. Hur, B. H. and S. J. Joo. 2003. Natural Products, pp.19-20, In Chang, I. M. and etc. (eds.), Treatise on Asian Herbal Medicines Vol II, Natural Products Research Institute, Seoul National University, Seoul
  12. Ju, I. O., G. T. Jung, J. Ryu, J. S. Choi, and Y. G. Choi. 2005. Chemical components and physiological activities of bamboo( Phyllostachys bambusoides Starf) extracts prepared with different methods. Korean J. Food Sci. Technol. 37, 542-548
  13. Jung, S., S. T. Kang, C. Y. Choi, K. Y. Oh, J. K. Cho, R. Rengasamy, and K. H. Park. 2009. Linoleic acid from bamboo (Phyllostachys Bambusoides) displaying potent α-glucosidase inhibition. Journal of Life Science 19, 680-683 https://doi.org/10.5352/JLS.2009.19.5.680
  14. Kim, H. S. and H. Yoon. 2008. Effects of chitosan oligosaccaride intake on the improvement of serum lipid level in hypercholesterolemic rats. Journal of Life Science 18, 1686-1692 https://doi.org/10.5352/JLS.2008.18.12.1686
  15. Kim, M. J., M. W. Byun, and M. S. Jang. 1996. Physiological and antibacterial activity of bamboo(Sasa coreana Nakai) leaves. J. Korean Soc. Food Sci. Nutr. 25, 135-142
  16. Kim, N. K., S. H. Cho, S. D. Lee, J. S. Ryu, and K. H. Shim. 2001. Functional properties and antimicrobial activity of bamboo(Phyllostachys sp.)extracts. Korean J. Postharvest Sci. technol. 8, 475-480
  17. Kim, S. Y., I. S. Su, and S. Y. Chung. 1993. Effects of the feeding Platycodon grandiflorum and Codonopsis lanceolata on the fatty acid composition of serum and liver in rats. J. Korean Soc. Food Nutr. 22, 524-530
  18. Kinnunen, P. K. J., J. Virtanen, and P. Vainio. 1983. Lipoprotein lipase and hepatic endothelial lipase. Atheroscler. Rev. 11, 65-99
  19. Kinsella, J. E. 1986. Dietary fish oil : possible effects of n-3 polyunsaturated fatty acids in reduction of thrombosis and heart disease. Nutrition Today 6, 7-14
  20. Kritchevsky, D. 1978. Fiber, lipids and atherosclerosis. Am. J. Clin. Nutr. 31, 365-374
  21. Lee, K. E. and G. H. Ryu. 2004. Change in chemical components of bamboo leaf powder extracts at different extraction conditions. Food Ind. Nutr. 9, 46-52
  22. Lee, M. J. and G. S. Moon. 2003. Antioxidative effects of Korean bamboo trees, wang-dae, som-dae, maengjong-juk, jolit-dae and o-juk. Korean J. Food Sci. Technol. 35, 1226-1232
  23. Lin, Y., M. A. Vermeer, and E. A. Trautwein. 2009. Triterpenic acids present in hawthorn lower plasma cholesterol by inhibiting intestinal ACAT activity in hamsters. eCAM 1-9
  24. Lorenz, J. P., V. Doormen, and K. F. Orlebeke. 1982. Stress, personality and serum cholesterol level. J. Human stress 8, 24-36
  25. Manttari, M., L. Tenkanen, and V. Manninen. 1994. A single total cholesterol measurement is a poor determinant of coronary risk. Atherosclerosis 109, 16-17
  26. McGrandy, R. B., D. M. Hegsted, and F. J. Stare. 1967. Dietary fat, carbohydrates and atherosclerotic vascular disease. New Engl. J. Med. 277, 242-247 https://doi.org/10.1056/NEJM196708032770505
  27. Miettinen, T. A. 1987. Dietary fiber and lipids. Am. J. Clin. Nutr. 45, 1237-1242
  28. Miller, M., C. P. Cannon, S. A. Murphy, J. Qin, K. K. Ray, E. Braunwald, and PROVE IT-TIMI 22 Investigators. 2008. Impact of triglyceride levels beyond low-density lipoprotein cholesterol after acute coronary syndrome in the PROVEIT-TIMI 22 Trial. Journal of the American College of Cardiology 51, 724-730 https://doi.org/10.1016/j.jacc.2007.10.038
  29. Oh, S. Y. and P. E. Monaco. 1985. Effect of dietary cholesterol and degree of fat unsaturation on plasma lipid levels, lipoprotein composition and fecal steroid excretion in normal young adult men. Am. J. Clin. Nutr. 42. 399-408
  30. Park, H. S., J. H. Lim, H. J. Kim, H. J. Choi, and I. S. Lee. 2007. Antioxidant flavone glycosides from the leaves of Sasa borealis. Arch. Pharm. Res. 30, 161-166 https://doi.org/10.1007/BF02977689
  31. Park, S. K., D. B. Choi, and H. Cho. 2008. A study on chemical components and biological activities of bamboo extract. J. Environ. Sani. Engin. 23, 65-70
  32. Phillips, N. R. and J. P. Kane. 1992. Levels and interrelationships of serum lipoprotein cholesterol and triglycerides. Association with adiposity and consumption of ethanol, tobacco, and beverages containing caffeine. Arteri. 1, 13-24
  33. Pratt, D. E. and B. W. Watts. 1964. The antioxidant activity of vegetable extracts, Flavone aglycones. J. food Sci. 29, 27-40 https://doi.org/10.1111/j.1365-2621.1964.tb01689.x
  34. Shin, M. K. and S. H. Han. 2002. Effects of methanol extracts from bamboo(Pseudosasa japonica Makino)leaves extracts on lipid metabolism in rats fed high cholesterol diet. Korean J. dietary Culture 17, 33-36
  35. Simopoulos, A. T. 1988. $\omega$-3 fatty acids in growth and development and the role of $\omega$-3 fatty acids in health and disease; dietary implication. Nutrition Today 10, 12-18
  36. Smith, E. B. 1974. The relationship between plasma and tissue lipid in human atherosclerosis. Adv. Lipid Res. 11, 1-7
  37. Wierzbicki, A. S. 2004. Lipid-altering agents: the future. Int. J. Clin. Pract. 58, 1063-1072 https://doi.org/10.1111/j.1742-1241.2004.00087.x
  38. Zhang, Y., J. Jiao, C. Liu, X. Wu, and Y. Zhang. 2008. Isolation and purification of four flavone C-glycosides from antioxidant of bamboo leaves by macroporous resin colum chromatography and preparative HPLC. Food Chemistry 107, 1326-1336
  39. Zhang, Y., X. Q. Wu, and Z. Y. Yu. 2002. Comparison study on total flavonoid content and anti-free radical activity of leaves of bamboo, Phyllostachys nigra and Ginko biloba. China J. Chinese Mat. Medica 27, 254-257

Cited by

  1. Quality Characteristics of Jeungpyun Prepared with Brown Rice and Sea Tangle Powder vol.32, pp.2, 2016, https://doi.org/10.9724/kfcs.2016.32.2.178
  2. Anti-Obesity Effect of Red Garlic Composites in Rats Fed a High Fat-Cholesterol Diet vol.22, pp.5, 2012, https://doi.org/10.5352/JLS.2012.22.5.671
  3. Effects of the Cnidium Officinale Makino Water Extract on the Lipid Metabolism of Rats Fed a Hypercholesterol Diet vol.26, pp.2, 2015, https://doi.org/10.7856/kjcls.2015.26.2.351
  4. Effect of Adding Barley Flour and Hizikia fusiformis Powder on Physicochemical and Sensory Characteristics of Jeungpyun vol.26, pp.4, 2016, https://doi.org/10.17495/easdl.2016.8.26.4.370
  5. Effects of Namhae Specialized Crops Water Extract on Lipid Metabolism in Rats Fed a Cholesterol Diet vol.27, pp.5, 2011, https://doi.org/10.9724/kfcs.2011.27.5.599
  6. Antioxidant activity and anti-obesity effect of Coprinus comatus in Zucker rat (fa/fa) vol.37, pp.1, 2014, https://doi.org/10.7853/kjvs.2014.37.1.51
  7. Effects of fermented blueberry liquid in high-fat diet-induced obese C57BL/6J mice vol.50, pp.6, 2017, https://doi.org/10.4163/jnh.2017.50.6.543