생명과학회지 (Journal of Life Science)

  • 제19권8호
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  • Pages.1033-1038
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Sphingomonas paucibilis NK-2000에 의한 젤란의 생산에 미치는 pH 안정제로서의 인산칼슐의 영향

The Effect of Potassium Phosphate as a pH Stabilizer on the Production of Gellan by Sphingomonas paucibilis NK-2000

  • 발행 : 2009.08.30
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초록

포도당 및 간장박의 농도가 각각 20 g/l 및 10 g/인 배지에서 Sphingomnas paucibilis NK2000가 생산하는 젤란의 최대 생산성은 배지의 초기 pH를 6.8로 하였을 경우, 플라스크 규모에서 7.46 g/l이었으며, 7 l 생물배양기에서는 7.35 g/l이었다. 배지의 pH를 6.8로 유지하면서 7 l 생물배양기에서 젤란을 생산할 때, 젤란의 최대 생산성은 pH 조절제로 수산화나트륨을 사용하였을 경우에 8.42 g/l 이었으며, 인산칼슘을 사용하였을 경우에 8.50 g/l이었다. Sphingomnas paucibilis NK2000를 배양하여 젤란을 생산할 경우에 배지에 첨가되는 인산칼슘의 최적 농도는 5.0 g/l이었다. 인산칼슘의 농도가 5.0 g/l인 배지를 사용하여 7 l 생물배양기에서 젤란을 생산하였을 때, 젤란의 최대 생산성은 8.93g/l이었다. 본 연구를 통하여 배지의 pH를 조절하지 않고 인산칼슘의 농도를 최적화한 배지를 사용하여 젤란의 생산성을 향상시킬 수 있는 경제적인 방법을 개발하였다.

Maximal productions of gellan by Sphingomnas paucibilis NK2000 from 20 g/l glucose and 10 g/l soybean pomace were 7.46 g/l in a flask and 7.35 g/l in a 7 l bioreactor, when the initial pH of media was 6.8. Maximal production of gellan in a 7 l bioreactor under pH control by sodium hydroxide was 8.42 g/l, whereas that under control by potassium phosphate was 8.50 g/l. The optimal concentration of potassium phosphate in a medium for production of gellan by S. paucibilis NK2000 was found to be 5.0 g/l. Maximal production of gellan in a medium containing 5.0 g/l potassium phosphate without pH control was 8.93 g/l in a 7 l bioreactor. In this study, a simple process without pH control was developed to enhance the production of gellan, with optimized concentration of potassium phosphate in the medium.

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