생명과학회지 (Journal of Life Science)

  • 제19권8호
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  • Pages.1003-1008
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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ITS에 의한 한국내 전복 속 분류군의 유전적 계통분류학적 연구

Phylogenetic Study of Genus Haliotis In Korea by Internal Transcribed Spacer Sequence (ITS)

  • Huh, Man-Kyu (Department of Molecular Biology, Dongeui University) ;
  • Kim, Jung-Ho (Biological Education, Busan National University) ;
  • Moon, Du-Ho (Biological Education, Busan National University)
  • 발행 : 2009.08.30
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초록

전복속에 속하는 종은 아시아를 포함한 세계에 광범위하게 서식한다. 전복속 종은 한국과 중국에서는 식용뿐만 아니라 약용으로도 이용된다. 한국내 전복속(genus Haliotis)에 속하는 분류군에 대해 ITS에 의한 계통관계를 조사하였다. 전복속 전체 종에서 5.8S exon은 160 핵산서열로 일정하였다. ITS1은 종에 따라 다양하였는데, 오분자기(H. diversicolor aquatilis)에서 272 핵산서열인 반면, 시볼트전복은 294 핵산서열이었다. ITS2 핵산서열 역시 종에 따라 다양하였다. 전체 서열은 오분자기는 722 핵산서열인 반면, 시볼트전복은 752 핵산서열이었다. ITS 전체 서열은 763 핵산서열에서 78개는 절약법에 정보적이었고, 57개는 변이로 비정보적이였고, 459는 일정하였다. 오분자기는 다른 전복속 종과 다른 분지를 나타내었다. ITS 서열로 한국내 분류군과 유럽종간 구분이 잘 되었다. ITS 서열로 종 동정에 이용할 수 있었으며, 종의 보전이나 생식질 보전에 기초로 이용될 수 있을 것으로 사료된다.

Abalone (genus Haliotis) is a woody species with a long life span that is primarily distributed throughout the world, including Asia. This species is regarded as a very important marine gastropod mollusk in Korea and China, and also in food industries around the world. We evaluated a representative sample of the five species with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within the genus. Aligned nucleotide sequences of the length of the 5.8S subunit of all taxa of Haliotis were found to constant of 160 bp nucleotides. However, aligned nucleotide sequences of the length of ITS1 were varied within genus Haliotis, varying from 272 in H. diversicolor aquatilis to 292 in H. discus hannai. Aligned nucleotide sequences of the length of ITS2, especially, vary from 722 in H. diversicolor aquatilis to 752 in H. sieboldii. Total alignment length is 763 positions, of which 78 are parsimony-informative, 57 variable but parsimony-uninformative, and 459 constant characters. H. discus hannai was similar to H. discus, while H. diversicolor aquatilis was more distinct. ITS analysis may be useful in germ-plasm classification several taxa of genus Haliotis.

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