Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antithrombotic and Cholesterol Reduction Effects of Defatted Soybean Grits Fermented by Bacillus subtilis NUC1

고초균에 의한 탈지대두 grits 발효물의 항혈전 및 콜레스테롤 저하 효과

  • Lee, Sung-Gyu (Department of Food and Technology, Keimyung University) ;
  • Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Im, Nam-Kyung (Department of Food and Technology, Keimyung University) ;
  • Lee, Eun-Ju (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Lee, Sam-Pin (Department of Food and Technology, Keimyung University) ;
  • Lee, In-Seon (Department of Food and Technology, Keimyung University)
  • 이성규 (계명대학교 식품가공학과) ;
  • 김현정 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터) ;
  • 임남경 (계명대학교 식품가공학과) ;
  • 이은주 ((주)엔유씨전자 바이오 연구소) ;
  • 이삼빈 (계명대학교 식품가공학과) ;
  • 이인선 (계명대학교 식품가공학과)
  • Published : 2009.08.31
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Abstract

This study investigated antithrombotic and hypocholesterolemic activities of defatted soybean grits (DSG) and fermented DSG (FD). The FD was prepared by the solid state fermentation using Bacillus subtilis NUC1 at $40^{\circ}C$ for 24h. The water extracts of fermented DSG (FDW) exhibited higher fibrinolytic activity and inhibition of platelet aggregation induced by ADP than water extracts of DSG (DW). However, the DW and FDW inhibited HMG-CoA reductase activity and significantly decreased the intracellular cholesterol contents in HepG2 cells. In addition, DW treatment did not show any cholesterol adsorption capacity, while FDW demonstrated the highest cholesterol adsorption by 90%. The results suggest that fermented DSG have significant antithrombotic and hypocholesterolemic effects in vitro and these activities were improved during fermentation by B. subtilis NUC1.

본 연구에서는 탈지대두 grits(DSG)와 B. subtilis NUC1균주로 발효시킨 탈지대두 발효물(FD)을 제조한 다음 열풍건조한 시료를 물 추출하여 이들 DSG 물추출물(DW)과 발효된 DSG 물추출물(FDW)의 항혈전 활성과 콜레스테롤 저하 효과를 알아보았다. 그 결과, FDW는 DW보다 항혈전 활성 및 혈소판의 응고 억제 효과가 더 우수하였으며, 유의적으로 높은 HMG-CoA reductase 저해활성을 가졌다. 그러나 DW와 FDW 처리군 둘다 HepG2 세포를 이용한 콜레스테롤 생합성 억제능을 보였고, 대조군에 비해 세포 내의 콜레스테롤 함량을 유의적으로 감소시켰다. 그리고 콜레스테롤 흡착능에서는 FDW만 약 90% 정도의 높은 흡착능을 보였고, DW는 콜레스테롤 흡착능을 보이지 않았다. 따라서 발효된 DSG는 원료인 DSG 보다 항혈전 및 콜레스테롤 개선 효과가 더 우수한 것으로 생각되었다.

Keywords

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