The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Neuroprotective Effect of PD-1 Extract in MPTP-lesioned Mouse Model of Parkinson's Disease

1-methyl-4-phenyl-1,2,3,6-tetrahydrophridine으로 유도된 파킨슨병 쥐에서의 도파민 신경세포 손상에 대한 PD-1 처방의 보호 효과

  • Lee, Jung-Wook (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Jung, Hye-Mi (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Seo, Un-Kyo (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University)
  • 이정욱 (동국대학교 한의과대학 내과학교실) ;
  • 정혜미 (동국대학교 한의과대학 내과학교실) ;
  • 서운교 (동국대학교 한의과대학 내과학교실)
  • Published : 2009.07.31
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Abstract

Objectives: The aim of the present study was to explore the neuroprotective effect and the possible mechanism of the PD-1 extracts on 1-methyl-4-phenyl-1,2,3,6-tetrahydrophridine (MPTP)-lesioned C57BL/6 mouse model of Parkinson's disease (PD). Methods: The mice were supplemented (or not) with 50 or 100 mg/kg/day of PD-1 for 2 weeks, after which MPTP was injected intraperitoneally. We observed that daily administration of PD-1 prevented MPTP-induced depletion of striatal DA, and maintained striatal and nigral tyrosine hydroxylase (TH) protein levels. Results: Our results demonstrated that mice treated with PD-1 prior to MPTP administration showed more abundant TH-immunopositive (TH-ir) fibers and neurons than mice given only MPTP, indicating that PD-1 protects dopaminergic striatal fibers and nigral neurons from MPTP insults. Possible neuroprotective effect of PD-1 was further studied by the detection of antiapoptotic protein (bcl-2) and proapoptotic protein (Bax). In this assay, MPTP elevated the Bax protein and decreased the bcl-2 protein, while these expressions were prevented by PD-1 pre-treatment. Conclusions: The present results suggest that PD-1 is able to protect dopaminergic neurons from MPTP-induced neuronal injury with anti-apoptotic activity being one of the possible mechanisms.

Keywords

References

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