생명과학회지 (Journal of Life Science)

  • 제19권7호
  • /
  • Pages.956-962
  • /
  • 2009
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

구강질환 원인균에 대한 자몽종자추출물과 법제유황수 함유 치약시제품의 향균효과

Antimicrobial Activity of Test Dentifrice Product Containing Grapefruit Seed Extract and Processed Sulfur Solution against Oral Pathogens

  • Lee, Bo-Bae (RIS Center, Industry-Academic Cooperation Foundation, Silla University) ;
  • Ha, Yu-Mi (RIS Center, Industry-Academic Cooperation Foundation, Silla University) ;
  • Shin, Su-Hwa (RIS Center, Industry-Academic Cooperation Foundation, Silla University) ;
  • Je, Kyoung-Mo (WellbuyKorea Co. Ltd.) ;
  • Kim, Soon-Rae (WellbuyKorea Co. Ltd.) ;
  • Choi, Jae-Suk (RIS Center, Industry-Academic Cooperation Foundation, Silla University) ;
  • Choi, In-Soon (RIS Center, Industry-Academic Cooperation Foundation, Silla University)
  • 발행 : 2009.07.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

구강질환 원인균에 대한 자몽종자추출물(GSE)과 법제유황수(PSS)의 항균활성을 확인하기 위해, 평판배지확산법 및 액체감수성실험법으로 Streptococcus mutans, Prevotella intermedia, Porphyromonas gingivalis 및 Candida albicans에 대한 항균활성을 측정하였다. 평판배지확산법의 경우, GSS의 농도가 40 ${\mu}l$/disk일 때, S. mutans, P. intermedia, P. gingivalis 및 C. albicans에 대한 항균활성은 각각 11.0, 9.5, 8.0, 9.0 mm로 나타났으며, PSS의 농도가 40 ${\mu}l$/disk일 때에는 각각 2.0, 3.5, 0.0, 1.5 mm로 나타났다. 또한 액체배지감수성실험에서 GSE에 대한 MIC 값은 0.24, 0.06, 0.10, 15.63 ${\mu}l$/ml 이었으며, PSS 에 대한 MIC 값은 각각 0.12, 3.91, >125, 7.81 ${\mu}l$/ml이었다. 치약 시제품의 항균효과를 확인하기 위해, GSE와 PSS가 농도별로 함유된 치약시제품 원액과 2배 및 4배 희석액을 제조하여 평판배지확산법을 수행한 결과, 0.5% 치약 시제품의 2배 희석액에서 각각 7.3, 4.3, 2.2 그리고 1.5 mm로 나타났다. 치약 시제품의 물리 화학적 특성을 확인한 결과, pH, 점도, 굴절 및 색도 변화에 있어서 모두 안정성을 보였고, 이상의 결과를 바탕으로 기능성 치약과 같은 구강질환 예방 및 치료제 개발 가능성을 확인하였다.

The aim of this study was to assess the effects of dentifrice-contatning grapefruit seed extract (GSE) and processed sulfur solution (PSS) on antimicrobial effects against oral pathogens. We first evaluated the antimicrobial effects of GSE and PSS against oral microbes: Streptococcus mutans (Sm), Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg) and Candida albicans (Ca). When antimicrobial activity against Sm, Pi, Pg and Ca was tested, at 40 $\mu$l/disk, the inhibition zones of GSE were 11.0, 9.5, 8.0 and 9.0 mm, respectively. With the same method, the inhibition zones of PSS were 2.0, 3.5, 0.0 and 1.5 mm, respectively. In the micro broth dilution method, the MIC values of GSE against Sm, Pi, Pg and Ca were 0.24, 0.06, 0.10 and 15.63 $\mu$l/rnl, respectively. The MIC values of PSS were 0.12, 3.91,>125 and 7.81 $\mu$l/ml, respectively. When pH, refractive index, viscosity and color value of dentifrice-containing GSE and PSS were measured, there were no significant changes in these physical properties compared to the control samples. Antimicrobial activities of dentifrice products containing 0.5% GSE and 0.5% PSS against oral pathogens were 7.3, 4.3, 2.2 and 1.5 mm, respectively. According to these results, we conclude that there may be a role for GSE and PSS in the development of new oral supplies.

키워드

참고문헌

  1. Bae, K. H., E. J. Jun, S. M. Lee, E. J. Lee, D. I. Paik, and J. B. Kim. 2005. The antimicrobial effect of CTS 50 chitosan on oral pathogenic microorganisms. J. Korean Acad. Dent. Health 29, 58-66
  2. Bae, K. H., S. H. Noh, and H. S. Moon. 2002. A study on the halitosis reduction by dentifrice containing triclosan, GFSE, eucalyptus oil and flavonoid. J. Korean Acad. Dent. Health 26, 251-257
  3. Block, E. 1986. The art and science. In folk medicine, pp. 125-137, In Steiver, R. P. (ed.), American Chemical Society, Washinton, DC
  4. Block, E. 1992. The organosulfur chemistry of the genus allium-Implications for the organic chemistry of sulfur. Angew Chem. Int. Fd. Engi. 31, 1135-1178 https://doi.org/10.1002/anie.199211351
  5. Chen, Y. T., R. L. Zheng, Z. J. Jia, and Y. Ju. 1990. Flavonoids as superoxide scavengers and antioxidants. Free Radic. Biol. Med. 9, 19-21
  6. Choi, J. D., I. W. Seo, and S. H. Cho. 1990. Studies on the antimicrobial activity of grapefruit seed extract. Bull. Korean Fish Soc. 23, 297-302
  7. Choi, K. H. and C. H. Kim. 2002. Growth inhibition of extract from sulfur fed duck caracass against various cancer cell lines. J. Korean Soc. Food Science of Animal Resources. 22, 348-351
  8. Cho, J. H., B. J. Min, O. S. Kwon, K. S. Shon, Y. G. Jin, H. J. Kim, and I. H. Kim. 2005. Effects of MSM (Methyl sulfonyl methane) supplementation on growth performance and digestibility of Ca and N in pigs. J. Korean Soc. Food Sci. Nutr. 34, 361-365 https://doi.org/10.3746/jkfn.2005.34.3.361
  9. Cho, M. J., S. J. Hong, C. H. Choi, and S. S. Jeong. Effects of dentifrice containing extract of Galla Rhois or Psoralea corylifolia on inhibition of plaque formation. J. Korea Acad. Dent. Health 29, 141-151
  10. Cho, S. H., I. W. Seo, J. D. Choi, and I. S. Joo. 1990. Antimicrobial and antioxidant activity of grapefruit and seed extract on fishery products. Bull. Korean Fish Soc. 23, 289-296
  11. de Soet, J. J., C. van Loveren, A. J. Lammens, M. J. Pavicić, C. H. Homburg, J. M. ten Cate, and J. de Graaff. 1991. Differences in cariogenicity between fresh isolates of Streptococcus sobrinus and Streptococcus mutans. Caries Res. 25, 116-122 https://doi.org/10.1159/000261353
  12. Filoche, S. K., K. Soma, and C. H. Sissons. 2005. Antimcrobial effects of essential oils in combination with chlorhexidine digluconate. Oral Microbiol. Immunol. 20, 221-225 https://doi.org/10.1111/j.1399-302X.2005.00216.x
  13. Hamada, N. and T. Takehara. 1984. Virulence factors of Streptococcus mutans and dental caries prevention. J. Dent. Res. 63. 407-411 https://doi.org/10.1177/00220345840630031001
  14. Hong, S. J., E. G. Choi, J. B. Son, and S. S. Jeong. Clinical effect of herbal extract containing dentifrice on the dental plaque and gingivitis. J. Korea Acad. Dent. Health 24, 171-183
  15. Hwang, S. J., S. N. Kim, S. Y. Chang, W. H. Ha, I. S. Kim, B. H. Jin, D. I. Paik, and H. D. Kim. 2005. Gingivitis suppression effect of the de novo dentifrice containing Curcuma xanthorrhiza, bamboo salt and various additives. J. Korean Acad. Dent. Health 29, 451-462
  16. Ishikawa, I., K. Nakashima, T. Koseki, T. Nagasawa, H. Watanabe, S. Arakawa, H. Nitta, and T. Nishihara. 1997. Induction of the immune response to periodontopathic bacteria and its role in the pathogenesis of periodontitis. Periodontol. 2000 14, 79-111 https://doi.org/10.1111/j.1600-0757.1997.tb00193.x
  17. Jagetia G. C. and T. K. Reddy. 2002. The grapefruit flavanone naringin protects against the radiation-induced genomic instability in the mice bone marrow: a micronucleus study. Mutat. Res. 519, 37-48 https://doi.org/10.1016/S1383-5718(02)00111-0
  18. Jang, Y. I. and T. R. Song. 2007. Sulphur solution containing and sulphur and method for manufacturing the same. Korea patent 10-2007-0000628
  19. Jung, S. H., K. H. Bae, H. S. Moon, D. I. Paik, S. H. Jung, and D. Y. Park. 1998. Effects of mouth spray containing GSE, tea extract & UDCA on antimicrobial effect of Streptococcus mutans, reducing of oral malodor and reduction of gingivitis. J. Korean Acad. Dent. Health 22, 37-46
  20. Kang, D. H., S. S. Chun, D. H. Chung, and S. H. Cho. 1994. Antimicrobial effect of grapefruit seed extract on vibrio parahaemolyticus isolated from the southern adjacent sea of Korea. J. Fd. Hyg. Safety 9, 141-149
  21. Kim, B. I., S. N. Kim., S. Y. Chang, K. T. Moon, Y. S. Kim, J. K. Hwang, S. H. Jeong, M. Y. Kim, H. S. Kim, and H. K. Kwon. 2005. A highly selective antibacterial effect of Curcuma xanthorrhiza extract against oral pathogens and clinical effectiveness of a dentifrice containing Curcuma xanthorrhiza extract for controlling bad breath. J. Korea Acad. Dent. Health 29, 222-237
  22. Kim, S. H. and Y. B. Seo. 1996. Effect of processed sulphur on experimental bone disease. Korean J. Oriental Medical Pathology. 10, 79-87
  23. Koh, C. M., K. H. Lee, J. Y. Park, M. C. Kim and D. S. Cha. 1997. Comparison of virulence factor expression between blood isolates of Candida albicans and commensal strain isolated from healthy volunteers. Korean J. Biotechnol. Bioeng. 32, 429-434
  24. Korea Food & Drug Administration. 2004. The regulation of inspection for declaration of item permission of medical supplies and others. No.2008-56
  25. Kumar, M. and J. S. Verwal. 1998. Sensitivity of food pathogens to garlic (Allium sativum). J. Appl. Microbiol. 84, 213-215 https://doi.org/10.1046/j.1365-2672.1998.00327.x
  26. Lang, N. P. and M. C. Brecx. 1986. Chlorhexidine digluconateab agent for chemical plaque control and prevention of gingival inflammation. J. Periodont. Res. 16, 74-89
  27. Lee C. H., T. S. Jeong, Y. K. Choi, B. H. Hyun, G. T. Oh, E. H. Kim, J. R. Kim, J. I. Han, and S. H. Bok. 2001. Anti-atherogenic effect of citrus flavonoids, naringin and naringenin, associated with hepatic ACAT and aortic VCAM-1 and MCP-1 in high cholesterol-fed rabbits. Biochem. Biophys. Res. Commun. 284, 681-688 https://doi.org/10.1006/bbrc.2001.5001
  28. Lee, S. L. and J. G. Kim. 2006. Anti-microbial activity of soybean extract against oral microbes. Kor. J. Env. Hlth. 32, 192-197
  29. Lee, T. E. 1987. Efficacy report of DF-100. Conference of genetics & cell biology. University of Malaya, Kuala Lumpur
  30. Lee, Y. S., S. G. Kim, T. C. Yang, G. S. Kim, J. G. Jeon, and K. W. Chang. 2006. The antibacterial and growth inhibitory effect of some essential oils against the oral micro-organisms. J. Korean Acad. Dent. Health 30, 490-496
  31. Loesche, W. J., J. Rowan, L. H. Straffon, and P. J. Loos. 1975. Association of Streptococcus mutants with human dental decay. Infect. Immun. 11, 1252-1260
  32. Mandalari, G., R. N. Bennett, G. Bisignano, D. Trombetta, A. Saija, C. B. Faulds, M. J. Gasson, and A. Narbad. 2007. Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamia Risso) peel, a byproduct of the essential oil industry. J. Appl. Microbiol. 103, 2056-2064 https://doi.org/10.1111/j.1365-2672.2007.03456.x
  33. Min, Y. K., J. K. Jeon, S. G. Kim, and K. W. Chang. 2001. Inhibitory effects of Schizandra chinensis extracts on the growth and adsorption to saliva - coated HA beads of some oral bacteria. J. Korean Acad. Dent. Health 25, 165-183
  34. National Committee for Clinical Laboratory Standards. 2002. Reference methods for broth dilution antifungal susceptibility testing of yeasts; Approved Standard-2nd eds., Vol.22, NCCLS Document M27-A2. Pennsylvania
  35. National Committee for Clinical Laboratory Standards. 2003. Reference methods for antimicicrobial disk susceptibility testing of anaerobic bacteria; Approved Standard-6th eds., Vol.23, NCCLS Document M2-A8. Pennsylvania
  36. National Committee for Clinical Laboratory Standards. 2003. Reference methods for antimicicrobial susceptibility testing of aerobic bacteria; Approved Standard-6th eds., Vol.23, NCCLS Document M7-A6. Pennsylvania
  37. National Committee for Clinical Laboratory Standards. 2004. Reference methods for antimicicrobial susceptibility testing of anaerobic bacteria; Approved Standard-6th eds., Vol.24, NCCLS Document M11-A6. Pennsylvania
  38. Pan, P. H., M. B. Finnegan, L. Sturdivant, and M. L. Barnett. 1999. Comparative antimicrobial activity of an essential oil and an amine fluoride/stannous fluoride mouthrinse in vitro. J. Clin. Periodontol. 26, 474-476 https://doi.org/10.1034/j.1600-051X.1999.260710.x
  39. Pirrs, G., R. Pianotti R, T. W. Feary, J. McGuiess, and T. Masurat. 1981. The in vivo effects of an antiseptic mouthwash on odorproducing microorganisms. J. Dent. Res. 60, 1891-1896 https://doi.org/10.1177/00220345810600111101
  40. Rauha, J. P., S. Remes, M. Heinonen, A. Hopia, M. Kahkonen, T. Kujala, K. Pihlaja, H. Vuorela, and P. Vuorela. 2000. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int. J. Food Microbiology 56, 3-12 https://doi.org/10.1016/S0168-1605(00)00218-X
  41. Read, M. A. 1995. Flavonoids: naturally occurring anti-inflammatory agents. Am. J. Pathol. 147, 235-237
  42. Reagor, L., J. Gusman, L. McCoy, E. Carino, and J. P. Heggers. 2002. The effectiveness of processed grapefruit-seed extract as an antibacterial agent: I. An in vitro agar assay. J. Altern. Complement Med. 8, 325-332 https://doi.org/10.1089/10755530260128014
  43. Renvert, S. and D. Birkhed. 1995. Comparison between 3 triclosan dentifrices on plaque, gingival and salivary microflora. J. Clin. Periodontol. 22, 63-70 https://doi.org/10.1111/j.1600-051X.1995.tb01772.x
  44. Song, I. S., D. H. Youn, and S. Y. Hwa, 2007. Effect of oral administration of processed sulphur on hepatotoxicity. Korean J. Oriental Physiology & Pathology. 21, 898-906
  45. Svatun, B., C. A. Saxton, and G. Rolla. 1990. Six-month study of effects of a dentifrice containing zinc citrate and triclosan on plaque, gingival health, and calculus. Scand. J. Dent. Res. 98, 301-304
  46. Tsui, V. W., R. W. Wong, and A. B. Rabie. 2008. The inhibitory effects of naringin on the growth of periodontal pathogenis in vitro. Phytother. Res. 22, 401-406 https://doi.org/10.1002/ptr.2338
  47. Wennstrom, J. and J. Lindhe. 1985. Some effects of a sanguinarine containing mouth wash on developing plaque and gingivitis. J. Clin. Periodontol. 12, 86-91

피인용 문헌

  1. Seed-surface disinfection and germination effects of grapefruit seed extract (GSE) on Lactuca sativa seeds vol.9, pp.2, 2017, https://doi.org/10.1007/s13530-017-0318-0
  2. Development of new antibacterial materials for manufacturing functional corrugated board for agricultural products vol.44, pp.3, 2012, https://doi.org/10.7584/ktappi.2012.44.3.034
  3. Antioxidant and Antimicrobial Activities of Xanthium sibiricum vol.25, pp.4, 2012, https://doi.org/10.7732/kjpr.2012.25.4.372
  4. Evaluation of Antibacterial Property and Freshness Maintenance of Functional Hybrid Corrugated Board Used for Agricultural Products vol.45, pp.3, 2013, https://doi.org/10.7584/ktappi.2013.45.3.045
  5. Antibacterial effect on leaf-extract from Nelumbo nucifera against oral microorganism vol.14, pp.1, 2014, https://doi.org/10.13065/jksdh.2014.14.01.117
  6. Growth inhibition effect ofRubus coreanusMiquel onCandida albicans vol.39, pp.3, 2015, https://doi.org/10.11149/jkaoh.2015.39.3.168
  7. Antibacterial Effect of Grapefruit Seed Extract (GSE) on Makgeolli-Brewing Microorganisms and Its Application in the Preservation of Fresh Makgeolli vol.79, pp.6, 2014, https://doi.org/10.1111/1750-3841.12469