Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Evaluation of Pollen Viability of Nakdongbyeo, Two Transgenic Rice Lines, Its Hybrids with Weedy Rice, and Subsequent Selfed Progenies: F2 and F3

낙동벼, 2개의 promoter를 각각 삽입한 유전자변형 계통과 잡초성벼(Oryza sativa)인공수정 한 후 다음세대인 F1, F2, F3의 화분활력 평가

  • Ghimire, Sita Ram (Division of Plant Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Sohn, Eun-Young (Division of Plant Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Shin, Dong-Hyun (Division of Plant Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, In-Jung (Division of Plant Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kim, Kil-Ung (Division of Plant Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • 기미어 시다람 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 손은영 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 신동현 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 이인중 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 김길웅 (경북대학교 농업생명과학대학 응용생명과학부)
  • Published : 2009.07.30
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Abstract

This experiment was conducted to evaluate pollen viability of Nakdongbyeo, transgenic rice lines, an F$_1$ hybrid from a cross between Milyang weedy rice and ABC-promoter transgenic rice line containing basta-resistant (bar) gene and subsequent selfed progenies, F$_2$ and F$_3$. The reaction of pollen with 3-{4,5 dimethylthiazolyl-2}-2,5-diphenyl monotetrazolium bromide (MTT) as a staining chemical immediately after pollen shedding showed maximum pollen viability of 86% in Nakdongbeyo, 75% in ABC-promoter transgenic rice line, 62% in ubiquitin-promoter transgenic line, 68% in F$_1$, 79% in F$_2$ and 78% in F$_3$. Viability gradually declined during subsequent observations at 20-minute intervals. However, there was a drastic decline in pollen viability after 40 minutes of pollen shedding. The mean difference of pollen viability among rice lines and time was highly significant, indicating significantly different pollen viabilities at different time intervals. Maximum viability of 36.2% was observed in F$_3$ and minimum viability of 3.5% was found in F$_2$ at 90 min after pollen shedding. Results of this experiment on pollen viability and longevity elucidate potential risks of pollen-mediated flow of herbicide-resistant gene from transgenic rice lines and possible integration of it into the weedy rice population.

본 연구는 비유전자변형 계통(낙동벼)과 2개의 다른 Promoter (aize ubiquitin과 abscisic acid)를 각각 삽입한유전자변형 벼와 abscisic acid promoter이용한 유전자변형 계통을 잡초성벼(Oryza sativa)와 인공수정 한 후 다음세대인 F$_1$, F$_2$, F$_3$의 활분활력, 형태형성 생장차이를 비교하여 평가하였다. 화분이 열개된 후 3-{4,5 dimethylthiazoly1-2}-2-,5-diphynly monotetrazolium bromide (MTT)반응을 살펴본 결과, Nakdongbeyo에서 86%, ABC-pro-moter 이용한 유전자변형 벼에서 75%, maize ubiquitin promoter이용한 유전자변형 벼에서 62%,F$_1$에서 68%, F$_2$에서 79% 및 F$_3$에서 78%의 각각 최대 화분활력을 보여주었다. 유전자변형 계통과 잡초성벼와의 교잡종의 F$_1$, F$_2$, F$_3$ 세대간의 화분활력을 비교하였을 때 화분 열개된 후 20분 까지는 유의한 차이를 보이지 않았으나, 40분에서 90분 사이에서는 F$_3$의 화분활력이 다른 두 세대 F$_1$, F$_2$보다 높게 나타났다. 화분을 열개된 90분 후 F$_3$ 세대 에서 최대 화분 활력이 36.2% 이었고 F$_2$ 세대 에서는 화분 활력이 최소 3.5% 보였다. 따라서 화분에 의해 벼의 유전자가 다른 계통으로 전이되는가를 조사하기 위하여 연차실험을 수행하였는데, 결론적으로 두 유전자변형 계통으로부터 잡초성벼로 유전자가 전이될 위험성이 나타날 것으로 보였다.

Keywords

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