Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

EFFECT OF DEXAMETHASONE CONCENTRATIONS ON OSTEOGENIC ACTIVITY OF CULTURED HUMAN PERIOSTEAL-DERIVED CELLS

배양된 인간 골막기원세포의 조골활성에 대한 덱사메타손 농도의 효과

  • Kim, Jong-Ryoul (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Park, Bong-Wook (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences) ;
  • Lee, Chang-Il (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences) ;
  • Hah, Young-Sool (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Kim, Deok-Ryong (Department of Biochemistry, Gyeongsang National University School of Medicine) ;
  • Cho, Yeong-Cheol (Department of Oral and Maxillofacial Surgery, College of Medicine, Ulsan University) ;
  • Sung, Iel-Yong (Department of Oral and Maxillofacial Surgery, College of Medicine, Ulsan University) ;
  • Byun, June-Ho (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences)
  • 김종렬 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 박봉욱 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원) ;
  • 이창일 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원) ;
  • 하영술 (경상대학교병원 임상의학연구소) ;
  • 김덕룡 (경상대학교 의학전문대학원 생화학교실) ;
  • 조영철 (울산대학교 의과대학 구강악안면외과학교실) ;
  • 성일용 (울산대학교 의과대학 구강악안면외과학교실) ;
  • 변준호 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원)
  • Published : 2009.07.31
Download PDF
⟨ Previous Next ⟩

Abstract

Long-term treatment with glucocorticoid leads to the development of osteoporosis and osteonecrosis. In contrast to the marked inhibitory effect of pharmacological doses of glucocorticoids on bone formation, the relationship between physiological concentrations of glucocorticoids and osteoprogenitor cell proliferation and phenotypes has not been elucidated yet. In addition, the effects of dexamethasone treatment on the proliferation and osteoblastic differentiation of osteoprogenitor cells are also controversial. The purpose of this study was to examine the effects of dexamethasone on the proliferation and osteoblastic differentiation of periosteal-derived cells. Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the cells were further cultured for 21 days in the osteogenic induction medium with different dexamethasone concentrations of 0, 10, and 100 nM. The proliferation and osteoblastic phenotypes of periosteal-derived cells were promoted in dexamethasone-treated cells than in untreated cells. Among the dexamethasone-treated cells, cell proliferation was slightly greater in 10 nM dexamethasone-treated cells than in 100 nM dexamethasone-treated cells. Histochemical staining and the bioactivity of alkaline phosphatase (ALP) were higher in 100 nM dexamethasone-treated cells than in 10 nM dexamethasone-treated cells. Similarly, von Kossa-positive mineralization nodules and calcium content were also more evident in 100 nM dexamethasone-treated cells than in 10 nM dexamethasone-treated cells. These results suggest that dexamethasone enhances the in vitro osteoblastic differentiation of periosteal-derived cells. The present study also demonstrates that higher dexamethasone concentrations reduce the in vitro proliferation of periosteal-derived cells.

Keywords

References

  1. Lukert BP, Johnson BE, Robinson RG : Estrogen and progesterone replacement therapy reduces glucocorticoidinduced bone loss. J Bone Miner Res 7 : 1063, 1992 https://doi.org/10.1002/jbmr.5650070909
  2. Bijlsma JW : Prevention of glucocorticoid induced osteoporosis. Ann Rheum Dis 56 : 507, 1997 https://doi.org/10.1136/ard.56.9.507
  3. Weinstein RS, Jilka RL, Parfitt AM et al : Inhibition of osteoblastogenesis and promotion of apoptosis of osteoblasts and osteocytes by glucocorticoids. Potential mechanisms of their deleterious effects on bone. J Clin Invest 102 : 274, 1998 https://doi.org/10.1172/JCI2799
  4. Canalis E : Mechanisms of glucocorticoid-induced osteoporosis. Curr Opin Rheumatol 15 : 454, 2003 https://doi.org/10.1097/00002281-200307000-00013
  5. Mikami Y, Omoteyama K, Kato S et al : Inductive effects of dexamethasone on the mineralization and the osteoblastic gene expressions in mature osteoblast-like ROS17/2.8 cells. Biochem Biophys Res Commun 362 : 368, 2007 https://doi.org/10.1016/j.bbrc.2007.07.192
  6. Mankin HJ : Nontraumatic necrosis of bone (osteonecrosis). N Engl J Med 326 : 1473, 1992 https://doi.org/10.1056/NEJM199205283262206
  7. Weinstein RS, Nicholas RW, Manolagas SC : Apoptosis of osteocytes in glucocorticoid-induced osteonecrosis of the hip. J Clin Endocrinol Metab 85 : 2907, 2000 https://doi.org/10.1210/jc.85.8.2907
  8. Leboy PS, Beresford JN, Devlin C et al : Dexamethasone induction of osteoblast mRNAs in rat marrow stromal cell cultures. J Cell Physiol 146 : 370, 1991 https://doi.org/10.1002/jcp.1041460306
  9. Cui Q, Wang GJ, Balian G : Steroid-induced adipogenesis in a pluripotential cell line from bone marrow. J Bone Joint Surg Am 79 : 1054, 1997 https://doi.org/10.2106/00004623-199707000-00012
  10. Atmani H, Audrain C, Mercier L et al : Phenotypic effects of continuous or discontinuous treatment with dexamethasone and/or calcitriol on osteoblasts differentiated from rat bone marrow stromal cells. J Cell Biochem 85 : 640, 2002 https://doi.org/10.1002/jcb.10165
  11. Jaiswal N, Haynesworth SE, Caplan AI et al : Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 64 : 295, 1997 https://doi.org/10.1002/(SICI)1097-4644(199702)64:2<295::AID-JCB12>3.0.CO;2-I
  12. Shui C, Spelsberg TC, Riggs BL et al : Changes in Runx2/Cbfα1 expression and activity during osteoblastic differentiation of human bone marrow stromal cells. J Bone Miner Res 18 : 213, 2003 https://doi.org/10.1359/jbmr.2003.18.2.213
  13. Sugiura F, Kitoh H, Ishiguro N : Osteogenic potential of rat mesenchymal stem cells after several passages. Biochem Biophys Res Commun 316 : 233, 2004 https://doi.org/10.1016/j.bbrc.2004.02.038
  14. Kotobuki N, Hirose M, Machida H et al : Viability and osteogenic potential of cryopreserved human bone marrowderived mesenchymal stem cells. Tissue Eng 11 : 663, 2005 https://doi.org/10.1089/ten.2005.11.663
  15. Mao X, Chu CL, Mao Z et al : The development and identification of constructing tissue engineered bone by seeding osteoblasts from differentiated rat marrow stromal stem cells onto three-dimensional porous nano-hydroxylapatite bone matrix in vitro. Tissue Cell 37 : 349, 2005 https://doi.org/10.1016/j.tice.2005.05.003
  16. Nakahara H, Goldberg VM, Caplan AI : Culture-expanded human periosteal-derived cells exhibit osteochondral potential in vivo. J Orthop Sci 9 : 465, 1991 https://doi.org/10.1002/jor.1100090402
  17. Breitbart AS, Grande DA, Kessler R et al : Tissue engineered bone repair of calvarial defects using cultured periosteal cells. Plast Reconstr Surg 101 : 567, 1998 https://doi.org/10.1097/00006534-199803000-00001
  18. Hutmacher DW, Sittinger M : Periosteal cells in bone tissue engineering. Tissue Eng 9(Suppl 1) : S45, 2003 https://doi.org/10.1089/10763270360696978
  19. Park BW, Byun JH, Lee SG et al : Evaluation of osteogenic activity and mineralization of cultured human periosteal-derived cells. J Kor Maxillofac Plast Reconstr Surg 28 : 511, 2006
  20. Park BW, Byun JH, Ryu YM et al : Correlation between vascular endothelial growth factor signaling and mineralization during osteoblastic differentiation of cultured human periosteal-derived cells. J Kor Maxillofac Plast Reconstr Surg 29 : 197, 2007
  21. Park BW, Hah YS, Kim DR et al : Osteogenic phenotypes and mineralization of cultured human periosteal-derived cells. Arch Oral Biol 52 : 983, 2007 https://doi.org/10.1016/j.archoralbio.2007.04.007
  22. Park BW, Hah YS, Kim DR et al : Vascular endothelial growth factor expression in cultured periosteal-derived cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 105 : 504, 2008 https://doi.org/10.1016/j.tripleo.2007.08.018
  23. Haussler MR, Manolagas SC, Deftos LJ : Glucocorticoid receptor in clonal osteosarcoma cell lines: a novel system for investigating bone active hormones. Biochem Biophys Res Commun 94 : 373, 1980 https://doi.org/10.1016/S0006-291X(80)80231-2
  24. Advani S, LaFrancis D, Bogdanovic E et al : Dexamethasone suppresses in vivo levels of bone collagen synthesis in neonatal mice. Bone 20 : 41, 1997 https://doi.org/10.1016/S8756-3282(96)00314-6
  25. Chavassieux P, Buffet A, Vergnaud P et al : Short-term effects of corticosteroids on trabecular bone remodeling in old ewes. Bone 20 : 451, 1997 https://doi.org/10.1016/S8756-3282(97)00016-1
  26. Abu EO, Horner A, Kusec V et al : The localization of the functional glucocorticoid receptor alpha in human bone. J Clin Endocrinol Metab 85 : 883, 2000 https://doi.org/10.1210/jc.85.2.883
  27. Carbonare LD, Arlot ME, Chavassieux P et al : Comparison of trabecular bone microarchitecture and remodeling in glucocorticoid-induced and postmenopausal osteoporosis. J Bone Miner Res 16 : 97, 2001 https://doi.org/10.1359/jbmr.2001.16.1.97
  28. Pereira RM, Delany AM, Canalis E : Cortisol inhibits the differentiation and apoptosis of osteoblasts in culture. Bone 28 : 484, 2001 https://doi.org/10.1016/S8756-3282(01)00422-7
  29. Porter RM, Huckle WR, Goldstein AS : Effect of dexamethasone withdrawal on osteoblastic differentiation of bone marrow stromal cells. J Cell Biochem 90 : 13, 2003 https://doi.org/10.1002/jcb.10592
  30. Igarashi M, Kamiya N, Hasegawa M et al : Inductive effects of dexamethasone on the gene expression of Cbfa1, Osterix and bone matrix proteins during differentiation of cultured primary rat osteoblasts. J Mol Histol 35 : 3, 2004 https://doi.org/10.1023/B:HIJO.0000020883.33256.fe
  31. Li X, Jin L, Cui Q et al : Steroid effects on osteogenesis through mesenchymal cell gene expression. Osteoporos Int 16 : 101, 2005 https://doi.org/10.1007/s00198-004-1649-7