Inhibition of an Inducible Nitric Oxide Synthase Expression by a Hexane Extract from Perilla frutescens cv. Chookyoupjaso Mutant Induced by Mutagenesis with Gamma-ray

방사선 유도 돌연변이 약용들깨 핵산 추출물의 Inducible Nitric Oxide Synthase 저해활성

  • Park, Yong Dae (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Min Ah (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Hyo Jung (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jin, Chang Hyun (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Dae Seong (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Dong Sub (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si-Yong (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Myung Woo (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jeong, Il Yun (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 박용대 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 강민아 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 이효정 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 진창현 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 최대성 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 김동섭 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 강시용 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 변명우 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 정일윤 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부)
  • Received : 2009.02.04
  • Accepted : 2009.02.20
  • Published : 2009.03.31

Abstract

In earlier investigations, seeds of Perilla frutescens(L.) Britt. cv. Chookyoupjaso were irradiated with 200 Gy gamma ray to generate mutagenesis. The aim of this study is to investigate the effects of a hexane extract from Perilla frutescens (L.) Britt. cv. Chookyoupjaso mutant 45 on the actions of anti-inflammatory activity on inducible nitric oxide synthase, and an identification of the major active compound. The hexane extract from P. frutescens exhibited activity of inhibition of a NO production ($IC_{50}$, $295.1{\mu}g\;ml^{-1}$). The hexane extract was further divided into subfractions by silica-gel chromatogarphy. Inhibition of the NO production by various fractions was assayed in LPS-stimulated RAW 264.7 cells. Among the seven fractions, the 5th fraction was the most effective ($IC_{50}$, $19.5{\mu}g\;ml^{-1}$). The 5th fraction suppressed the expression of protein of iNOS in LPS-induced RAW 264.7 cells, and GC/MS analyses showed that isoegomaketone is a major bio-active compound in the 5th fraction. The result indicated that isoegomaketone has a good potential to be developed as an anti-inflammation agent.

Keywords

Acknowledgement

Supported by : 농림수산식품부