Annals of Clinical Neurophysiology

The Korean Society of Clinical Neurophysiology (대한임상신경생리학회)
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Development of a Discogenic Pain Animal Model: Preliminary Study

추간판성통증 동물모델의 개발: 초기 연구

  • Kim, Byung-Jo (Department of Neurology, Korea University College of Medicine) ;
  • Lee, Min (Department of Biomedical Science, Korea University Graduate School) ;
  • Lim, Eun-Jeong (Department of Physiology, Korea University College of Medicine) ;
  • Yu, Sung-Wook (Department of Neurology, Korea University College of Medicine) ;
  • Hong, Sung-Ha (Department of Neurology, Korea University College of Medicine) ;
  • Hong, Seok-Joo (Department of Radiology, Korea University College of Medicine) ;
  • Na, Heung-Sik (Department of Physiology, Korea University College of Medicine)
  • 김병조 (고려대학교 의과대학 신경과학교실) ;
  • 이민 (고려대학교 의과대학 의학대학원) ;
  • 임은정 (고려대학교 의과대학 생리학교실) ;
  • 유성욱 (고려대학교 의과대학 신경과학교실) ;
  • 홍성하 (고려대학교 의과대학 신경과학교실) ;
  • 홍석주 (고려대학교 의과대학 영상의학교실) ;
  • 나흥식 (고려대학교 의과대학 생리학교실)
  • Received : 2009.04.14
  • Accepted : 2009.07.28
  • Published : 2009.12.30
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Abstract

Background: Discogenic pain can develop into chronic low back pain that is very difficult to treat effectively, because the pathogenesis of the disease still remains controversial. To clarify the pathogenesis, numerous animal models of intervertebral disc degeneration have been proposed in the literature, each with attendant advantages and disadvantages. The aim of this study was to determine the most efficacious method and dose of complete Freund's adjuvant (CFA) injection into intervertebral disc to develop a discogenic pain in a rat. Methods: CFA was injected into the L5-L6 or L4-L5 disc of male Sprague-Dawley rats in various conditions including a dose of CFA (10, 20, or 50 uL), drilling, injection site sealing using cyanoacrylate, and injection velocity. Sham animals were subjected to the same procedure, except for the CFA injection. Mechanical and heat allodynia were serially measured at both hindpaws until 8 weeks post-operatively. Serial MRI analyses were performed to observe degenerative changes of the discs. In addition, CGRP & Substance P-immunoreactivities (ir) in the superficial dorsal horn were evaluated at 4 weeks using immunohistochemistry. Results: Each condition provoked various problems such as development of hindpaw paralysis, CFA leakage, and no pain development. Mid-sagittal T2 MRI revealed no significant degenerative changes in the CFA injected disc. The CGRP-ir of the bilateral superficial dorsal horns at the level of L5-L6 was significantly increased in the CFA group. Conclusions: A total of 10 uL CFA injection into L5-L6 disc for a period of 10 minutes using a 26-gauge needle without drilling was the most efficacious way to develop discogenic pain animal model.

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