Effect of Heat Treatment on the Lipophillic Pigments of Fresh Green Tea Liquor

  • Published : 2009.06.30

Abstract

Changes in lipophillic pigments concentration and its relation to color of fresh green tea liquor during heat treatment were studied. The results showed liquor greenness decreased markedly with extension of incubation time at $55^{\circ}C$, while the brightness and yellowness changed a little. Significant increase in 'a' and 'b' values of tea liquor was observed at $95^{\circ}C$. Color change of liquor at $55^{\circ}C$ was accompanied by a decrease in the level of chlorophylls, lutein and neoxanthin, and an increase in the pheophytins and ${\beta}-carotene$ levels. However, all pigments except ${\beta}-carotene$ decreased with time extension at $95^{\circ}C$. Significant correlation was found between pigments and color difference index. The browning of fresh green tea liquor was attributed to vicissitudes of lipophillic pigments during heat treatment, especially to the change of chlorophylls/pheophytins ratio. Result also showed addition of $Zn^{2+}$ at 1.6 ${\mu}mol/L$ could partially alleviate the decrease in greenness during heat treatment.

Keywords

References

  1. Panza VSP, Wazlawik E, Schutz GR, Comin L, Hecht KC, Silva EL. Consumption of green tea favorably affects oxidative stress markers in weight-trained men. Nutrition 24: 433-442 (2008) https://doi.org/10.1016/j.nut.2008.01.009
  2. Nagle DG, Ferreira D, Zhou YD. Epigallocatechin-3-gallate (EGCG): Chemical and biomedical perspectives. Phytochemistry 67: 1849-1855 (2006) https://doi.org/10.1016/j.phytochem.2006.06.020
  3. Gupta S, Saha B, Giri AK. Comparative antimutagenic and anticlastogenic effects of green tea and black tea: A review. Mutat. Res. 512: 37-65 (2002) https://doi.org/10.1016/S1383-5742(02)00024-8
  4. Fujiki H, Suganuma M, Imai K, Nakachi K. Green tea: Cancer preventive beverage and/or drug. Cancer Lett. 188: 9-13 (2002) https://doi.org/10.1016/S0304-3835(02)00379-8
  5. Khan N, Mukhtar H. Multitargeted therapy of cancer by green tea polyphenols. Cancer Lett. 269: 269-280 (2008) https://doi.org/10.1016/j.canlet.2008.04.014
  6. Khan N, Mukhtar H. Tea polyphenols for health promotion. Life Sci. 81: 519-533 (2007) https://doi.org/10.1016/j.lfs.2007.06.011
  7. Kim ES, Liang YR, Jin J, Sun QF, Lu JL, Du YY, Lin C. Impact of heating on chemical compositions of green tea liquor. Food Chem. 103: 1263-1267 (2007) https://doi.org/10.1016/j.foodchem.2006.10.031
  8. Liang YR, Lu JL, Zhang LY. Comparative study of cream in infusions of black tea and green tea [Camellia sinensis (L) O. Kuntze]. Int. J. Food Sci. Tech. 37: 627-634 (2002) https://doi.org/10.1046/j.1365-2621.2002.00589.x
  9. Wang LF, Kim DM, Lee CY. Effects of heat processing and storage on flavanols and sensory qualities of green tea beverage. J. Agr. Food Chem. 48: 4227-4232 (2000) https://doi.org/10.1021/jf0003597
  10. Yamanishi T, Hara Y, Luo S, Wickremasinghe RL. Special issue on tea. Food Rev. Int. 11: 371-546 (1995) https://doi.org/10.1080/87559129509541049
  11. Manzocco L, Anese M, Nicoli MC. Antioxidant properties of tea extracts as affected by processing. Lebensm. -Wiss. Technol. 31: 694-698 (1998) https://doi.org/10.1006/fstl.1998.0491
  12. Cichelli A, Pertesana GP. High-performance liquid chromatographic analysis of chlorophylls, pheophytins and carotenoids in virgin olive oils: Chemometric approach to variety classification. J. Chromatogr. A 1046: 141-146 (2004) https://doi.org/10.1016/S0021-9673(04)01061-1
  13. Chen BH, Liu MH. Relationship between chlorophyll a and $\beta$-carotene in a lipid-containing model system during illumination. Food Chem. 63: 207-213 (1998) https://doi.org/10.1016/S0308-8146(98)00006-5
  14. Cortes C, Esteve MJ, Frígola A. Color of orange juice treated by high intensity pulsed electric fields during refrigerated storage and comparison with pasteurized juice. Food Control 19: 151-158 https://doi.org/10.1016/j.foodcont.2007.03.001
  15. Tonucci LH, Von Elbe JH. Kinetics of the formation of zinc complexes of chlorophyll derivatives. J. Agr. Food Chem. 40: 2341-2344 (1992) https://doi.org/10.1021/jf00024a004
  16. LaBorde LF, von Elbe JH. Chlorophyll degradation and zinc complex formation with chlorophyll derivatives in heated green vegetables. J. Agr. Food Chem. 42: 1100-1103 (1994) https://doi.org/10.1021/jf00041a010
  17. Canjura FL, Watkins RH, Schwartz SJ. Color improvement and metallo-chlorophyll complexes in continuous flow aseptically processed peas. J. Food Sci. 64: 987-990 (1999) https://doi.org/10.1111/j.1365-2621.1999.tb12265.x
  18. Rodriguez-Amaya DB. A Guide to Carotenoid Analysis in Foods. ILSI Press, Washington, DC, USA. pp. 41-45 (2001)
  19. Pan SS, Lu JL, Yang XL, Zheng XQ, Du YY, Devijat B, Liang YR. Study on HPLC method for the analysis of trace pigments in readyto-drink tea. J. Tea Sci. 27: 343-346 (2007)
  20. Liang YR, Lu JL, Zhang LY, Wu S, Wu Y. Estimation of tea quality by infusion color differences analysis. J. Sci. Food Agr. 85: 286-292 (2005) https://doi.org/10.1002/jsfa.1953
  21. Kong F, Tang J, Rasco B, Crapo C. Kinetics of salmon quality changes during thermal processing. J. Food Eng. 83: 510-520 (2007) https://doi.org/10.1016/j.jfoodeng.2007.04.002
  22. Yamauchi N, Harada K, Watada AE. In vitro chlorophyll degradation in stored broccoli (Brassica oleracea L. var. italica Plen.) florets. Postharvest Biol. Tec. 12: 239-245 (1997) https://doi.org/10.1016/S0925-5214(97)00063-X
  23. Sankat CK, Maharaj V. Shelf life of the green herb 'shado beni' (Eryngium foetidum L.) stored under refrigerated conditions. Postharvest Biol. Tec. 7: 109-118 (1996) https://doi.org/10.1016/0925-5214(95)00016-X
  24. Lessin WJ, Catigani GL, Schwartz SJ. Quantification of cis-trans isomers of provitamin A carotenoids in fresh and processed fruits and vegetables. J. Agr. Food Chem. 45: 3728-3732 (1997) https://doi.org/10.1021/jf960803z
  25. Lamberts L, Rombouts I, Delcour JA. Study of nonenzymic browning in $\alpha$-amino acid and $\gamma$-aminobutyric acid/sugar model systems. Food Chem. 111: 738-744 (2008) https://doi.org/10.1016/j.foodchem.2008.04.051
  26. Bozkurt H, Gogus F, Eren S. Nonenzymic browning reactions in boiled grape juice and its models during storage. Food Chem. 64: 89-93 (1999) https://doi.org/10.1016/S0308-8146(98)00081-8
  27. Roig MG, Bello JF, Rivera ZS, Kennedy JF. Studies on the occurrence of non-enzymatic browning during storage of citrus juice. Food Res. Int. 32: 609-619 (1999) https://doi.org/10.1016/S0963-9969(99)00128-3
  28. Schamberger GP, Labuza TP. Effect of green tea flavonoids on Maillard browning in UHT milk. Food Sci. Technol.-Leb. 40: 1410-1417 (2007) https://doi.org/10.1016/j.lwt.2006.09.009
  29. Liang HL, Liang YR, Dong JJ, Lu JL. Tea extraction methods in relation to control of epimerization of tea catechins. J. Sci. Food Agr. 87: 1748-1752 (2007) https://doi.org/10.1002/jsfa.2913
  30. Es-Safi NE, Cheynier V, Moutounet M. Implication of phenolic reactions in food organoleptic properties. J. Food Compos. Anal. 16: 535-553 (2003) https://doi.org/10.1016/S0889-1575(03)00019-X
  31. Hyvarinen K, Hynninen PH. Liquid chromatographic separation and mass spectrometric identification of chlorophyll b allomers. J. Chromatogr. A 837: 107-116 (1999) https://doi.org/10.1016/S0021-9673(99)00059-X
  32. Eckhardt U, Grimm B, Hortensteiner S. Recent advances in chlorophyll biosynthesis and breakdown in higher plants. Plant Mol. Biol. 56: 1-14, 20 (2004) https://doi.org/10.1007/s11103-004-2331-3
  33. World Health Organization and Food and Agriculture Organization of the United Nations. 2nd ed. Vitamin and Mineral Requirements in Human Nutrition. Report of a Joint FAO/WHO LC/NLM Classification: QU 145, WHO Press: Geneva, Switzerland. pp. 230-243 (2004)