Food Science and Biotechnology

  • 제18권3호
  • /
  • Pages.667-671
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  • 2009
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  • 1226-7708(pISSN)
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  • 2092-6456(eISSN)
한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

Characteristics of Water-soluble Polysaccharide, Showing Inhibiting Activity on ${\alpha}-Glucosidase$, in Cordyceps militaris

  • Chung, Ha-Yull (Department of Food Science & Biotechnology, Food & Biotechnology Research Center, Hankyong National University) ;
  • Yoo, Mi-Kyong (Department of Food Science & Biotechnology, Food & Biotechnology Research Center, Hankyong National University) ;
  • Kawagishi, Hirokazu (Department of Applied Biological Chemistry, Shizuoka University)
  • 발행 : 2009.06.30
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초록

Extract of water-soluble polysaccharide (CFWx), showing inhibiting activity on ${\alpha}-glucosidase$, was prepared from the fruiting bodies of Cordyceps militaris by hot-water extraction, and ethanol precipitation. Chemical characteristics of CFWx were as follows: carbohydrate content 30% including 16% of uronic acid; 51% protein content; monosaccharide composition, Man:Glu:Gal (30:43:27); molecular weight $3-5{\times}10^4$. CFWx was further purified by ion-exchange, gel-permeation, and affinity chromatography and $CFWx-AH-{\alpha}$ fraction was isolated. Fundamental structure of $CFWx-AH-{\alpha}$ was deduced as ${\alpha}-(1{\to}4$)-D-glucan with ${\alpha}-(1{\to}3$)- and/or ${\alpha}-(1{\to}6$)-D-glycosidic side chains based on methylation analysis.

키워드

참고문헌

  1. Groop LC. Sulfonylureas in NIDDM. Diabetes Care 15: 737-754 (2000) https://doi.org/10.2337/diacare.15.6.737
  2. DeFronzo RA, Goodman AM. Efficacy of metformin in patients with non-insulin-dependent diabetes mellitus: The multicenter metformin study group. New Engl. J. Med. 333: 541-549 (1995) https://doi.org/10.1056/NEJM199508313330902
  3. Bailey CJ. Biguanides and NIDDM. Diabetes Care 15: 755-772 (1992) https://doi.org/10.2337/diacare.15.6.755
  4. Ying J, Mao X, Ma Q, Zong Y, Wen H. Icons of Medical Fungi from China. Science Press, Beijing, China. pp. 60-85 (1987)
  5. Kiho T, Hui J, Yamane A, Ukai S. Hypoglycemic activity and chemical properties of a polysaccharide from the cultural mycelium of Cordyceps sinensis. Biol. Pharm. Bull. 16: 1291-1293 (1993) https://doi.org/10.1248/bpb.16.1291
  6. Kiho T, Yamane A, Hui J, Usui S, Ukai S. Hypoglycemic activity of a polysaccharide (CS-F30) from the cultural mycelium of Cordyceps sinensis and its effect on glucose metabolism in mouse liver. Biol. Pharm. Bull. 19: 294-296 (1996) https://doi.org/10.1248/bpb.19.294
  7. Watanabe J, Kawabata J, Kurihara H, Niki R. Isolation and identification of $\alpha$-glucosidase inhibitors from tochu-cha. Biosci. Biotech. Bioch. 61: 177-178 (1997) https://doi.org/10.1271/bbb.61.177
  8. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356 (1956) https://doi.org/10.1021/ac60111a017
  9. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275 (1951)
  10. Taylor KA, Buchanan-Smith JG. A colorimetric method for the quantitation of glucuronic acids and a specific assay for galacturonic acid. Anal. Biochem. 201: 190-196 (1992) https://doi.org/10.1016/0003-2697(92)90194-C
  11. Blakeney AB, Harris PJ, Henry RJ, Stone BA. A simple and rapid preparation of alditol acetates for monosaccharide analysis. Carbohyd. Res. 113: 291-299 (1983) https://doi.org/10.1016/0008-6215(83)88244-5
  12. Narui T, Takahashi K, Kobayashi M, Shibata S. Permethylation of polysaccharides by a modified Hakomori method. Carbohyd. Res. 103: 293-295 (1982) https://doi.org/10.1016/S0008-6215(00)80692-8
  13. Kwon YM, Cho SM, Kim JH, Lee JH, Lee YA, Lee SJ, Lee MW. Hypoglycemic effect of Cordyceps militaris. Korean J. Pharmacogn. 32: 327-329 (2001)
  14. Kim DH, Choi HJ, Bae EA, Han MJ, Park SY. Effect of artificially cultured Phellinus linteus on harmful intestinal bacterial enzymes and rat intestinal $\alpha$-glucosidases. J. Food Hyg. Saf. 13: 20-23 (1998)
  15. Kiho T, Ookubo K, Usui S, Ukai S, Hirano K. Structual features and hypoglycemic activity of a polysaccharide (CS-F10) from the cultured mycelium of Cordyceps sinensis. Biol. Pharm. Bull. 22: 966-970 (1999) https://doi.org/10.1248/bpb.22.966
  16. Janson P, Kenne L, Liedgren H, Lindberg B, Lonngren J. A practical guide to the methylation analysis of carbohydrates. Chem. Commun. 8: 26-74 (1976)
  17. Chaplin MF. Monosaccharides. Chap. 1, pp. 33-35. In: Carbohydrate Analysis. Chaplin MF, Kennedy JF (eds). IRL Press. Oxford. England (1986)
  18. Chung HY, Cho YJ, Kim T. Isolation and characterization of a water-soluble polysaccharide from the mycelia of solid cultivated Agaricus blazei Murill. Food Sci. Biotechnol. 14: 259-262 (2005)
  19. Chung HY, Kim T. Isolation and characterization of a water-soluble polysaccharide from the mycelia of solid cultivated Phellinus linteus. Food Sci. Biotechnol. 14: 783-787 (2005)
  20. Kim JE, Lee WS, Chung HY, Jang SJ, Kim JS, Lee JB, Song CS, Park SY. The selective antitumor activity of water-soluble extracts of the fruiting bodies and the cultivated mycelia of Agaricus blazei Murill. Food Sci. Biotechnol. 13: 347-352 (2004)