한국한의학연구원논문집 (Korean Journal of Oriental Medicine)

한국한의학연구원 (Korean Institute of Oriental Medicine)
권호 표지

HPLC를 이용한 발효 감초의 지표 성분 분석

Quantitative Analysis of Glycyrrhizic Acid in Fermented Glycyrrhizae Radix by HPLC

  • 엄영란 (한국한의학연구원 신한방제제연구센터) ;
  • 심기석 (한국한의학연구원 신한방제제연구센터) ;
  • 이재훈 (한국한의학연구원 신한방제제연구센터) ;
  • 박화용 (한국한의학연구원 신한방제제연구센터) ;
  • 마진열 (한국한의학연구원 신한방제제연구센터)
  • Um, Young-Ran (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Shim, Ki-Shuk (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Lee, Jae-Hoon (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Park, Hwa-Yong (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine)
  • 투고 : 2009.02.28
  • 심사 : 2009.04.17
  • 발행 : 2009.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The aim of this study was to study the quantitative analysis of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Paecilomyces japonica, Ganoderma lucidum, honey or Nuruk. The amounts of dry on loss were measured and the quantitative analysis of glycyrrhizic acid was performed by high performance liquid chromatographic (HPLC). HPLC method was performed on C18 column ($250\;mm\;{\times}\;4.6\;mm$, $5\;{\mu}m$, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (254 nm). The flow rate was $1.0\;m{\ell}/min$. Retention time of glycyrrhizic acid was about 23.96 min and linearity of calibration was $R^2$=0.9998. Contents of glycyrrhizic acid in Glycyrrhizae Radix extract (control) was $5.048\;{\pm}\;0.14$; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Paecilomyces japonica (SDT) was $1.975\;{\pm}\;0.07$; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Ganoderma lucidum (SYT) was $2.676 \;{\pm}\;0.07$; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with honey (SST) was $5.191\;{\pm}\;0.06$; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Nuruk (SNT) was $5.305\;{\pm}\;0.34$, respectively. Contents of glycyrrhizic acid in SDT and SYT were decreased but that in SST and SNT was increased when compared to control.

키워드

참고문헌

  1. 한국약학대학협의회 약전분화회, 대한약전 제8개정, 서울:신일상사, 2003:1056-7.
  2. 중약대사전편찬위원회, 완역 중약대사전, 서울:정담, 1999:66-78.
  3. 생약학연구회, 현대생약학, 서울:학창사, 1992:274-276.
  4. Asl MN, Hosseinzadeh H., Review of pharmacological effects of Glycyrrhiza sp, and its bioactive compounds, Phytother Res., 2008;22:709-724. https://doi.org/10.1002/ptr.2362
  5. Németh K, Plumb GW, Berrin JG, Juge N, Jacob R, Naim HY, Williamson G, Swallow DM, Kroon PA, Deglycosylation by small intestinal epithelial cell beta-glucosidases is a critical step in the absorption and metabolism of dietary flavonoid glycosides in human, Eur J Nutr., 2003;42:29-42. https://doi.org/10.1007/s00394-003-0397-3
  6. 안덕균, 김호철, 한약포제학, 서울:일중사, 2000: 147-151.
  7. 동의학연구소, 동양법제, 서울:여강출판사, 1994:61-64.
  8. 최혁재, 이우정, 박성환, 송보완, 김동현, 김남재, 한약수치에 대한 연구-초감초 제법의 표준화 및 규격화, 생약학회지, 2005;36:209-219.
  9. Ha BJ, Ku CS, Bae DJ, Hwang KS, Kim CH, Jang DI, Bio-conversion of liquiritin and liquiritin apioside from Glycyrrhiza uralensis root to liquiritigenin during nuruk fermenation, 한국생물공학회 국제 심포지움, 한국생물공학회, 2009.
  10. 나인수, 박민주, 노종훈, 민진우, 방면호, 양덕춘, 발효법제에 의한 감초의 Flavonoid 무배당체의 생산, 동의생리병리학회지, 2008;22:569-574.
  11. Izumi T, Piskula MK, Osawa S, Obata A, Tobe K, Saito M, Kataoka S, Kubota Y, Kikuchi M, Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans, J Nutr, 2000;130:1695-1699. https://doi.org/10.1093/jn/130.7.1695
  12. Hasegawa H, Proof of the mysterious efficacy of ginseng: basic and clinical trials: metabolic activation of ginsenoside: deglycosylation by intestinal bacteria and esterification with fatty acid, J Pharmacol Sci, 2004;95:153-157. https://doi.org/10.1254/jphs.FMJ04001X4
  13. 손미에, 버섯균사체로 발효시킨 복령과 후박의 항산화 및 항암효과, 식품산업과 영양, 2007;12:51-57.
  14. Bae EA, Trinh HT, Rhee YK, Antiallergic effect of ginseng fermented with Ganoderma lucidum, J Gingseng Res, 2008;32:57-61. https://doi.org/10.5142/JGR.2008.32.1.057
  15. Shin HS, Ustunol Z, Carbohydrate composition of honey from different floral sources and their influence on growth of selected intestinal bacteria: An in vitro comparison, Food Res Int, 2005;38: 721-728. https://doi.org/10.1016/j.foodres.2005.01.007
  16. 식품의약품안전청, 대한약전 제9개정, 서울:신일북스, 2008:1125.
  17. 식품의약품안전청, 대한약전 제9개정, 서울:신일북스, 2008:903-904.
  18. 최혁재, 이우정, 박성환, 송보완, 김동현, 김남재, 한약수치에 관한 연구(제5보)-초감초 제법의 표준화와 규격화 (I), 생약학회지, 2005;36:209-219.