Quantitative Analysis of Glycyrrhizic Acid in Fermented Glycyrrhizae Radix by HPLC

HPLC를 이용한 발효 감초의 지표 성분 분석

  • Um, Young-Ran (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Shim, Ki-Shuk (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Lee, Jae-Hoon (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Park, Hwa-Yong (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine)
  • 엄영란 (한국한의학연구원 신한방제제연구센터) ;
  • 심기석 (한국한의학연구원 신한방제제연구센터) ;
  • 이재훈 (한국한의학연구원 신한방제제연구센터) ;
  • 박화용 (한국한의학연구원 신한방제제연구센터) ;
  • 마진열 (한국한의학연구원 신한방제제연구센터)
  • Received : 2009.02.28
  • Accepted : 2009.04.17
  • Published : 2009.04.30

Abstract

The aim of this study was to study the quantitative analysis of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Paecilomyces japonica, Ganoderma lucidum, honey or Nuruk. The amounts of dry on loss were measured and the quantitative analysis of glycyrrhizic acid was performed by high performance liquid chromatographic (HPLC). HPLC method was performed on C18 column ($250\;mm\;{\times}\;4.6\;mm$, $5\;{\mu}m$, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (254 nm). The flow rate was $1.0\;m{\ell}/min$. Retention time of glycyrrhizic acid was about 23.96 min and linearity of calibration was $R^2$=0.9998. Contents of glycyrrhizic acid in Glycyrrhizae Radix extract (control) was $5.048\;{\pm}\;0.14$; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Paecilomyces japonica (SDT) was $1.975\;{\pm}\;0.07$; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Ganoderma lucidum (SYT) was $2.676 \;{\pm}\;0.07$; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with honey (SST) was $5.191\;{\pm}\;0.06$; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Nuruk (SNT) was $5.305\;{\pm}\;0.34$, respectively. Contents of glycyrrhizic acid in SDT and SYT were decreased but that in SST and SNT was increased when compared to control.

Keywords

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