Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Fermented Crataegi fructus Vinegar Improves Lipid Metabolism in Rats Fed High Fat Diet

산사발효초가 고지방식이를 급여한 흰쥐의 지질대사에 미치는 영향

  • Chon, Jeong-Woo (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Park, Jin-Kyung (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Mi-Ae (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Jeong, Mi-Ran (Research Center of Bioactive Materials, Chonbuk National University) ;
  • Han, Jong-Hyun (Dept. of Herbal Professional, Graduate School of Oriental Medicine, Won Kwang University) ;
  • Park, Yoo-Kyoung (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
  • 전정우 (경희대학교 동서의학대학원 의학영양학과) ;
  • 박진경 (경희대학교 동서의학대학원 의학영양학과) ;
  • 이미애 (경희대학교 동서의학대학원 의학영양학과) ;
  • 정미란 (전북대학교 생리활성물질연구소) ;
  • 한종현 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 박유경 (경희대학교 동서의학대학원 의학영양학과)
  • Published : 2009.08.31
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Abstract

Crataegi fructus has been used as an oriental medicine and a folk remedy for the treatment of scurvy, constipation and stomach ailment. This study was aimed to investigate the effect of fermented Crataegi fructus vinegar (FCV) on the improvement of lipid metabolism in rats fed high fat diet (40% kcal% fat, fat source; beef tallow). Sprague-Dawley rats (n=32) were randomly divided into four groups [normal diet (ND), high fat diet (HD), and high fat diet supplemented with low (CFL; 1.5% wt/wt) and high (CFH; 3.0% wt/wt) doses of fermented Crataegi fructus vinegar] and were fed experimental diets for 6 weeks. At the end of the experiment, the body weight in high fat diet groups was higher than that of normal diet group, while the body weights of CFL and CFH group were significantly reduced by 7.2% and 10.0%, respectively, as compared with that of HD group. Moreover, liver and kidney weights in CFL and CFH group were significantly lower than that of HD group (p<0.05). The levels of serum triglyceride, total cholesterol, LDL-C, atherogenic index and hepatic triglyceride, total cholesterol in CFH group were significantly decreased as compared with HD group whereas it increased the serum level of HDL-C than HD group (p<0.05). CFL and CFH groups showed significantly decreased AST, ALT and ALP of serum as compared with HD group. Excretions of fecal saturated fatty acid in CFH group was significantly increased compared with ND and HD groups. These results imply that fermented Crataegi fructus vinegar can be used as possible food resources and functional food materials.

본 연구에서는 산사 발효초가 고지방식이를 급여한 흰쥐의 지질대사에 미치는 영향에 대해 살펴보았다. 흰쥐에게 고지방식이(40% kcal% fat, beef tallow)를 급여하면서 산사발효초를 저농도(1.5% wt/wt, n=8), 고농도(3.0% wt/wt, n=8)로 투여하였을 때, 정상식이군(n=8)과 고지방식이 대조군(n=8)과의 체중, 장기무게, 지질 함량 등의 차이를 관찰하였다. 체중은 산사발효초군이 정상식이군보다 유의적으로 증가하였지만, 고지방식이 대조군보다 유의적으로 각각 7.2%, 10.0% 감소하였다. 간과 신장 무게도 고지방식이 대조군에 비해 산사발효초군들에서 유의적으로 감소하였다. 고농도 산사발효초군에서 혈청의 중성지방, 총콜레스테롤, LDL-C, 동맥경화지수 및 간의 중성지방, 총콜레스테롤 함량은 고지방식이 대조군에 비해 유의적으로 감소하였지만 혈청 중 HDL-C 함량은 유의적으로 증가하였다. 분변 중 포화지방산 함량은 고농도 산사발효초군에 비해 정상식이군이나 고지방식이대조군에서 유의적으로 높았다. 결론적으로 산사발효초는 체중을 감소시키며 혈청이나 간의 지질함량을 개선시키고, 포화지방산 배설을 증가시키므로 체중조절 및 지질대사 개선 효과가 규명되었다.

Keywords

References

  1. Kopelman PG. 2005. Obesity as a medical problem. Nature 404: 635-643 https://doi.org/10.1038/35007508
  2. Lee SK, So SH, Hwang EI, Koo BS, Han GH, Ko SB, Kim NM. 2008. Effect of ginseng and herbal plant mixtures on anti-obesity in obese SD rat induced by high fat diet. J Korean Soc Food Sci Nutr 37: 437-444 https://doi.org/10.3746/jkfn.2008.37.4.437
  3. Ahn IS, Park KY, Do MS. 2007. Weight control mechanisms and antiobesity functional agents. J Korean Soc Food Sci Nutr 36: 503-513 https://doi.org/10.3746/jkfn.2007.36.4.503
  4. George AB, Louis AT. 2000. Medicinal strategies in the treatment of obesity. Nature 404: 672-677 https://doi.org/10.1038/35007544
  5. Jeong TS, Hwang EI, Lee HB, Lee ES, Kim YK, Min BS, Bae KH, Bok SH, Kim SU. 1999. Chitin synthase Ⅱ inhibitory activity of ursolic acid, isolated from Crataegus pinnatifida. Planta Med 65: 261-263 https://doi.org/10.1055/s-2006-960474
  6. 허준. 2001. 동의보감. 근영출판사, 서울. p 325
  7. Kim JS, Lee GD, Kwon JH, Yoon HS. 1993. Antioxidative effectiveness of ether in Crataegus pinnatifida Bunge and Terminalia chebula rats. J Korean Agric Chem Soc 36: 203-207
  8. Kang IH, Cha JH, Lee SW, Kim HJ, Kwon SH, Han IH, Hwang BS, Whang WK. 2005. Isolation of anti-oxidant from domestic Crataegus pinnatifida Bunge leaves. Kor J Parmacogn 36: 121-128
  9. Lee HJ, Choi MS. 1999. Measurement of inhibitory activities on 3-hydroxy-3-methylglutaryl CoA reductase and Acyl-CoA: Cholesterol acyltransferase by various plant extracts in vitro. J Korean Soc Food Sci Nutr 28: 958-962
  10. Kao ES, Wang CJ, Lin WL, Yin YF, Wang CP, Tseng TH. 2005. Anti-inflammatory potential of flavonoid contents from dried fruit of Crataegus finnatifida in vitro and in vivo. J Agric Food Chem 53: 430-436 https://doi.org/10.1021/jf040231f
  11. Seo BI. 2005. Preventive effects of water extracts from on Crataegi fructus on hyperlipiderma and liver damage induced by alcohol. Kor J Herbology 20: 35-43
  12. Min BS, Jung HJ, Lee JS, Kim YH, Bok SH, Ma CM, Nakamura N, Hattori M, Bae K. 1999. Inhibitory effect of triterpenes from Crataegus pinnatifida on HIV-I protease. Planta Med 65: 374-375 https://doi.org/10.1055/s-2006-960792
  13. Hong SS, Hwang JS, Lee SA, Han XH, Ro JS, Lee KS. 2002. Inhibitors of monoamine oxidase activity from the fruits of Crataegus pinnatifida Bunge. Kor J Pharmacogn 33: 285-290
  14. Jeong YJ, Lee MH, Seo KI, Kim JN, Lee YS. 1998. The quality comparison of grape vinegar by two stages fermentation with traditional grape vinegar. J East Asin Soc Dietary Life 16: 731-739
  15. Kim MK, Kim MY, Youn EK, Kim SD. 2002. Extraction of citrus bioflavonoid with vinegars and effects on blood pressure. Korean J Food Preserv 9: 411-417
  16. Kim DH, Lee JS. 2000. Vinegar production from subtropical fruits. J Korean Soc Food Sci Nutr 29: 68-75
  17. Yae MJ, Lee GH, Nam KH, Jang SY, Woo SM, Jeong YJ. 2007. Establishment of quality control standardization for pomegranate vinegar. J Korean Soc Food Sci Nutr 36: 1425-1430 https://doi.org/10.3746/jkfn.2007.36.11.1425
  18. Kim DH. 1999. Studies on the production of vinegar from fig. J Korean Soc Food Sci Nutr 28: 53-60
  19. Kwon SH, Jeong EJ, Lee GD, Jeong YJ. 2000. Preparation method of fruit vinegars by two stage fermentation and beverages including vinegar. Food Industry and Nutrition 5: 18-24
  20. Shin JS, Lee OS, Jeong YJ. 2002. Changes in the components of onion vinegars by two stage fermentation. Korean J Food Sci Technol 34: 1079-1084
  21. 한국식품공업협회. 2002. 식품공전. 훈영사, 서울. p 452-455
  22. AOAC. 1980. Official Method of Analysis. 12th ed. Association of Official Analytical Chemist, Washington, DC, USA
  23. Prosky L, Asp NG, Furda I, Deuries JW, Schweizer TF, Harland BF. 1984. Determination of total dietary fiber in foods, food products and total diets. JAOAC 67: 1044-1052
  24. Friedewald WT, Levy RI, Fredrickson DS. 1979. Estimation of concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-502
  25. Yim JE, Choue RW, Kim YS, Oh SJ, Peang JR. 2000. Dietary and simvastatin treatment on the blood lipid levels in the patients with hyperlipidemia according to genetic and biochemical markers. Kor J Lipidol 10: 215-229
  26. Folch J, Lees M, Sloane Stanley GH. 1957. A simple method for isolation and purification of total lipids from animal tissue. J Biol Chem 226: 497-509
  27. Cho YS, Jang EM, Jang SM, Chun MS, Shon MY, Kim MJ, Lee MK. 2007. Effect of grape seed water extract on lipid metabolism and erythrocyte antioxidant defense system in high-fat diet-induced obese C57BL/6 mice. J Korean Soc Food Sci Nutr 36: 1537-1543 https://doi.org/10.3746/jkfn.2007.36.12.1537
  28. Wursch P. 1979. Influence of tannin-rich carob pob fiber on the cholesterol metabolism in the rat. J Nutr 109: 685-692 https://doi.org/10.1093/jn/109.4.685
  29. Jung YJ, Han DO, Choi BH, Park C, Lee HJ, Kim SH, Hahm DH. 2007. Effect of fermented herbal extracts, HP-1 on enzyme activities and gene expressions related to alcohol metabolism in ethanol-loaded rats. Korean J Oriental Physiology & Pathology 21: 387-391
  30. Shon MY. 2007. Antioxidant and anticancer activities of Poria cocos and Machilus thunbergii fermented with mycelial mushrooms. Food Industry and Nutrition 12(2): 51-57
  31. Stephen CW, David AD, Patrick T, Paul AR, Deborah JC, Stephen CB, Koro G, Min L, Randy JS. 2004. Consumption of a high-fat diet alters the homeostatic regulation of energy balance. Physiol Behav 83: 573-578 https://doi.org/10.1016/j.physbeh.2004.07.026
  32. Stewart LK, Soileau JL, Ribnicky D, Wang ZQ, Raskin I, Poulev A, Majewski M, Cefalu WT, Gettys TW. 2008. Quercetin transiently increases energy expenditure but persistently decreases circulating markers of inflammation in C57BL/6J mice fed a high-fat diet. Metabolism 57: 39-46 https://doi.org/10.1016/j.metabol.2008.03.003
  33. Gordon T, Kannel WB, Castelli WP, Dawber TR. 1981. Lipoproteins, cardiovascular disease, and death. Arch Inter Med 141: 1128-1131 https://doi.org/10.1001/archinte.141.9.1128
  34. Jung SH, Kim JH, Jeong YJ, Choi MJ. 1999. Effect of persimmon vinegar on serum lipid profile in rats with high cholesterol diet. J East Asian Soc Dietary Life 9: 421-426
  35. Rhee SJ, Kim KR, Kim HT, Hong JH. 2007. Effects of catechin on lipid composition and adipose tissue in obese rats fed high fat diet. J Korean Soc Food Sci Nutr 36: 540-547 https://doi.org/10.3746/jkfn.2007.36.5.540
  36. Sudhahar V, Ashokkumar S, Varalakshmi P. 2006. Effect of lupeol and lupeol linoleate on lipemic-hepatocellular aberrations in rats fed a high cholesterol diet. Mol Nutr Food Res 50: 1212-1219 https://doi.org/10.1002/mnfr.200600134
  37. Glaber G, Graefe EU, Struck F, Veit M, Gebhard R. 2002. Comparison of antioxidative capacities and inhibitory effects on cholesterol biosynthesis of quercetin and potential metabolites. Phytomedicine 9: 33-40 https://doi.org/10.1078/0944-7113-00080
  38. Ahn JY, Lee HJ, Kim SN, Park JH, Ha TY. 2008. The anti- obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways. Biochem Biophys Res Commun 373: 545-549 https://doi.org/10.1016/j.bbrc.2008.06.077
  39. Zhang Z, Ho KK, Huang Y, Chen ZY. 2002. Hypocholesterolemic activity of hawthorn fruit is mediated by regulation of cholesterol-7α-hydroxylase and acyl CoA: cholesterol acyltransferase. Food Res Intern 35: 885-891 https://doi.org/10.1016/S0963-9969(02)00099-6

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