Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Thiosulfinates Isolated from Allium tuberosum L. on the Growth of Human Cancer Cells

부추의 함황화합물이 인체 암세포 증식에 미치는 영향

  • Park, Sun-Young (Dept. of Food and Nutrition, Sunchon National University) ;
  • Kim, Jae-Yong (Dept. of Food Science and Technology, Kyungpook National University) ;
  • Park, Kyung-Wuk (Dept. of Food and Nutrition, Sunchon National University) ;
  • Kang, Kap-Suk (Dept. of Hotel Culinary Arts, Busan College of Information Technology) ;
  • Park, Ki-Hun (Dept. of Environmental Biotechnology, Gyeongsang National University) ;
  • Seo, Kwon-Il (Dept. of Food and Nutrition, Sunchon National University)
  • 박순영 (순천대학교 식품영양학과) ;
  • 김재용 (경북대학교 식품공학과) ;
  • 박경욱 (순천대학교 식품영양학과) ;
  • 강갑석 (부산정보대학 호텔조리과) ;
  • 박기훈 (경상대학교 환경생명화학과) ;
  • 서권일 (순천대학교 식품영양학과)
  • Published : 2009.08.31
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Abstract

To develop Allium tuberosum L. as a cancer preventive food material, thiosulfinates and biological active components were isolated from Allium tuberosum L. and the apoptotic effects of thiosulfinates in human cancer cells were examined. Thiosulfinates decreased viable cell numbers in dose- and time-dependent manners. Thiosulfinates at the 20 $\mu g$/mL concentration inhibited more than 60% cell proliferation in HepG2 and A549 human cancer cells, respectively. Also the morphology of cells treated with thiosulfinates of 30 $\mu g$/mL concentration was distorted with shrunken cell mass while the cell number was lower than that of control cells. The $IC_{50}$ values in the HepG2 cells were higher than those of the A549 cells. Thiosulfinates at the 30 $\mu g$/mL concentration showed the formation of apoptotic bodies and a nuclear condensation, and an increase in the cell populations of the sub-G1 phase in the HepG2 cells. These results indicate that thiosulfinates from Allium tuberosum L. inhibited cell proliferation in HepG2 via apoptosis.

부추를 암 예방 식품 소재로 활용하기 위하여 부추로부터 주요 생리활성 물질인 함황화합물을 분리하여 인체 암세포성장 억제 및 간암세포의 사멸이 apoptosis에 의해 유도되는지를 조사하였다. 부추 함황화합물을 1, 5, 10, 20 및 30 $\mu g$/mL 농도로 24, 48 및 72시간별로 간암(HepG2) 및 폐암세포 (A549)에 처리하여 암세포 증식억제 효과를 측정한 결과 HepG2 및 A549 세포에서 농도 및 시간 의존적으로 그 성장을 억제하였으며, 20 $\mu g$/mL 농도 이상에서 암세포 성장이 60% 이상 억제되었다. 또한 부추 함황화합물 30 $\mu g$/mL 농도로 처리 시 대조군에 비하여 폐암 및 간암 세포수 감소 및 심한 형태학적 변화가 관찰되었다. 이들 암세포의 $IC_{50}$ 값을 측정한 결과 부추 함황화합물은 폐암세포(A549)보다 간암세포(HepG2)에 더 효과가 있었다. 한편 부추 함황화합물은 30 $\mu g$/mL 농도에서 핵 응축 및 apoptotic body를 나타내었으며, 농도 의존적으로 subG1 DNA 함량이 증가함으로써 HepG2 암세포 사멸이 apoptosis에 의해 유도되는 것을 확인할 수 있었다.

Keywords

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