Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Considering Cell-based Assays and Factors for Genome-wide High-content Functional Screening

  • Chung, Chul-Woong (Creative Research Initiative (CRI)-Acceleration Research, School of Biological Science/Bio-Max Institute, Seoul National University) ;
  • Kim, In-Ki (LG Life Science Research Park) ;
  • Jung, Yong-Keun (Division of Applied Molecular Oncology, Ontario Cancer Institute)
  • Published : 2009.06.30
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Abstract

Recently, great advance is achieved in the field of genome-wide functional screening using cell-based assay. Here, we briefly introduce well-established and typical cell-based assays of GPCR and some parameters which should be considered for genome-wide functional screening. Because of characters and importance of GPCR as drug targets, several ways of assay systems were devised. Among them, high-content screening (HCS) that is based on the analysis of image by confocal microscope is becoming favorite choice. The advances in this technology have been driven exclusively by industry for their convenience. Now, it is turn for academy to define more detail signaling networks via HCS using cDNA or siRNA libraries at genome-wide level. By isolating novel signaling mediators using cDNA or siRNA library, and postulating them as new candidates for therapeutic target, more understanding about life science and more increased chances to develop therapeutics against human disease will be achieved.

Keywords

References

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