산업기술연구 (Journal of Industrial Technology)
- 제29권A호
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- Pages.169-176
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- 2009
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- 1229-9588(pISSN)
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- 1598-1371(eISSN)
Bacillus licheniformis NBL420 유래의 Xylanase 유전자의 클로닝과 특성 검토
Cloning and Characterization of Xylanase Gene from Bacillus licheniformis NBL420
초록
The gene encoding endoxylanase (xylS) was isolated from a genomic library of Bacillus licheniformis NBL420. Two positive clones, which harbor 1.5 kb and 0.8 kb inserts respectively, were screened on RBB dyed-xylan plates and the recombinant plasmids were named as pBX3 and pBX5. The nucleotide sequencings of two inserts revealed the existence of common 639 bp of open reading frame which encode 232 amino acids. The xylS gene was successfully subcloned into pET22b(+) vector and overexpressed. Enzymatic properties including optimum pH, optimum temp, thermostability and pH stability were investigated. Activity staining of XylS was identical with that of original Bacillus licheniformis NBL420.