Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지

Biochemical characteristics of Brucella abortus isolated from cattle in Gyungbuk province

경북지방 소에서 분리한 Brucella abortus의 생화학적 특성

  • Published : 2009.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Bovine brucellosis is a zoonosis, long incubation period and chronic infectious disease, usually caused by Brucella abortus. This study was carried out to investigate the biotyping and biochemical characterization of B. abortus isolated from 208 farm 871 korean cattle and holstein diagnosed brucellosis by serological positive in Gyeongbuk province during the period from 2002 to 2006. B. abortus was isolated from 124 (14.2%) of 871 cattle, and isolated 110 (13.4%) of 820 Korean cattle and 14 (27.5%) of 51 holstein in breed. The uterus of korean cattle was isolated in 8 (17.8%) of 45 cattle and supramammary lymph none of holstein was isolated 11 (68.8%) of 16 cattle. 101 (12.5%) of 810 serological positive blood samples were isolated B. abortus. The isolation rate of B. abortus was correlated with antibody titers. The biochemical characterization of isolates was non-hemolytic, production of H$_2$S, oxidase-positive, catalase-positive, hydrolyzation of urea and growth of basic fuchsin dye medium. As a result, all of isolates was identified B. abortus bv 1. 124 isolates were susceptible to ampicillin, lincospectin, amikacin, gentamicin, kanamycin, neomycin, streptomycin, tetracycline, ciprofloxacin, norfloxacin and enrofloxacin.

Keywords

References

  1. 김두희, 정병택, 문재봉 1968. 브루셀라감염한우에 대한 혈청학적연구. 농사시험연구보고 11(5): 1-18
  2. 김병구, 김동성, 이택주. 1957. Brucellosis에 관한 연구- 제1보 Brucellosis의 면역학적 조사보고. 가축위생연구소 연구보고 5: 79-91
  3. 김병구, 송병균, 이택주. 1959. Brucellosis 에 관한 연구. 제2보 가축 brucellosis 에 대한 면역학적 조시보고. 가축위생연구소연구보고 6: 9-23
  4. 김우택, 이완수, 김공식. 1991 제주도내 축우 부푸셀아병 발생상황 조사. 한국가축위생학회지 14(2): 104-109
  5. 김종만, 우승룡, 이지연, 정석찬, 강승원. 김종염, 윤용덕, 조상래, 유한상, Olson Sc. 2000. 부루서1라백산(RB51) 의 안전성에 관한 연구. 대한수의학회지 40(3): 533-541
  6. 김종만, 정석찬, 박정문, 현관종, 마점술. 1988. 부루셀라 양성우에서 분리한 부루셀라균의 성상과 혈청학적 진단법 비교. 농사시험연구논문집 30(2): 1-6
  7. 깅종성 1997. 소 부루세라병 잠복기간과 방역대책, 제주도 소 부부세라병 근절대책을 중심으로. 한국가축위생학회지 20(1): 1-10
  8. 농림수산식품부. 2007. 결핵 및 브루셀라병 방역실시요령. 농림부
  9. 박노찬, 김상윤, 조광현, 김영환, 조민희, 정종식, 감봉환. 1998. 경북지역의 부루셀라병에 관한 연구. 한국가축위생학회지 21(4): 451-465
  10. 삼헝섭, 고태오, 유성종, 우종태, 박병옥, 김성렬, 박유순. 1996. 경기도에서 발생하는 유우부루셀라병에 관한 연구. 한국가축위생학회지 19(3): 189-198
  11. 정종식, 조용준, 박청규. 1988. 경북지방 젖소로부터 Brucella abortus의 분리 빛 균형별. 대한수의학회지 28(2): 339-343
  12. Alton GG, Jones LM, Piet DE. 1975. Laboraton techniques in brucellosis. 2nd Ed. WHO, Geneva: 11-63
  13. Bannatyne RM, Jackson MC, Memish Z. 1997. Rapid diagnosis of brucella bacteremia by using the BA-CTEC 9240 System. J Clin MicrobioI 35(10): 2673-2674
  14. Beran GW, Steele JH. 1994. Handbook of zoonoses. 2nd Ed. CRC Press, Boca Raton: 9-39
  15. Brenner DJ, Kricg NR, Staley JT. 2005. Bergey’s manual of systemιltic bacteriology. 2nd Ed, Vol 2. Springer Prcss, New York: 370-389
  16. Clinical and Laboratory Standards Institute. 2008. Performance standards for antimicrobial disk and dilution susceptibiliy tests for bacteria isolated from animals. 3rd. CLSI document M31-A3, Wayne, Pennsylvania
  17. Douglas JT, Rosenberg EY, Nikaido H, Verstreate DR, Winter AJ. 1984. Porins of brucella Species. Infect lmmun 44(1): 16-21
  18. Etemadi H, Raissadat A, Pickett J, Vahedifar P. 1984. Isolation of brucella spp. from clinical specimens. J Clin Microbiol 20(3); 586
  19. Farrell ID. 1974. The development of a new selεctive medium for the isolation of Brucella abortus from contaminated sources. Res Vet Sci 16: 280-286
  20. Fiori PL, Mastrandrca S, Rappelli P, Cappuccinelli P. 2000 Brucella abortus infection acquired in microbology laboratories. J Clin Microbiol 38(5): 2005-2006
  21. Garcia-Rodriguez JA, Munoz Bellido JL, Fresnadillo MJ, Trujillano I. 1993. In vitro activities of new macrolides and rifapεntine against brucella spp. Antimicrob Agents Chemother 37(4): 911-913 https://doi.org/10.1128/AAC.37.4.911
  22. Garcia-Rodriguez JA, Garcia Sanchez JE, Trujillano I. 1991. Lack of effectivc bactricidal activity of new quinolones against brucella spp. Antimicrob Agents Chemother 35(4): 756-759 https://doi.org/10.1128/AAC.35.4.756
  23. Gaviria-Ruiz MM, Cardona-Castro NM. 1995. Evaluation and comparison of different blood culture techniqucs for bacteriological isolation of Salmonella typhi and Brucella abortus. J Clin MicrobioI 33(4): 868-871
  24. Hall WH, Manion RE. 1970 In vitro susceptibility of brucella to various antibiotics. Appl MicrobioI 20(4) 600-604
  25. Hirsh DC, Zee YC. 1999. Veterinary microbiology. Blackwell Seience Press: 196-203
  26. Hnber JD, Nicoletti P. 1986. Comparison of the results of card, rivanol, complement-fixation, and milk ring tests with the isolation rate of Brucella abortus from cattle. Am J Vet Res 47(7): 1629-1631
  27. Lopez-Goni I, Moriyon I. 2004. Brucella: Molecular and cellular biology. Horizon Bioscience, Norfolk: 1-432
  28. Meyer KF, Eddie B. 1941. Laboratory infections due to Brucella. J Infect Dis 68: 24-32 https://doi.org/10.1093/infdis/68.1.24
  29. Nielsen K, Duncan JR. 1990. Animal brucellosis. CRC Press, Boston: 1-453
  30. OIE. 2004. Manual of diagnostic test and vaccines for terrestrial animals. Part 2, Section 2.3, Chapter 2.3.1. OIE Press, France, Paris
  31. Richardson M, Holt JN. 1962. Synergistic action of streptomycin with other antibiotics on intracellu1ar Brucella abortus in vitro. J Bacteriol 84; 638-646
  32. Robichaud S, Libman M, Behr M, Rubin E. 2004. Prevention of laboratory-acquired Brucellosis. Clin Infect Dis 38: 119-122 https://doi.org/10.1086/421024
  33. Santos JM, Verstreate DR, Perera VY, Winter AJ. 1984. Outer membrane proteins from rough strains of four brucella species. Infect lmmun 46(1): 188-194
  34. Schurig GG, Martin Roop II R, Bagchi T, Boyle S, Buhrman D, Srirangnathan N. 1991. Biological properties of RB51. a stable rough strain of Brucella abortus. Vet Microbiol 28: 171-188 https://doi.org/10.1016/0378-1135(91)90091-S
  35. Timoney JF, Gillespie JH, Scott FW, Barlough JE. 1988. Hagan and Bruner’s Microbiology and infectious disease of domestic animals. 8th Ed. Cornell University Press, New York: 135-152
  36. Verstreate DR, Winter AJ. 1984. Comparison of sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles and antigenic relatedness among outer membrane proteins of 49 Brucella abortus strains. lnfect Immun 46(1): 182-187
  37. Wilesmith JW. 1978. The persistence of Brucella abortus infection in calves: a retrospective study of heavil infected herds. Vet Rec 103(8): 149-153 https://doi.org/10.1136/vr.103.8.149
  38. Young EJ, Corbel MJ. 1989. Brucellosis: Clinical and laboratory aspects. CRC Press, Boca Raton: 1-192