Macromolecular Research

  • 제17권7호
  • /
  • Pages.538-543
  • /
  • 2009
  • /
  • 1598-5032(pISSN)
  • /
  • 2092-7673(eISSN)
한국고분자학회 (The Polymer Society of Korea)
권호 표지

Methotrexate-Incorporated Polymeric Micelles Composed of Methoxy Poly(ethylene glycol)-Grafted Chitosan

  • Jeong, Young-Il (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Seo, Dong-Hyuk (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Kim, Don-Gon (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Choi, Chang-Yong (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Jang, Mi-Kyeong (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Nah, Jae-Woon (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Park, Yoon-Kyung (Department of Biotechnology and BK21 Research Team for Protein Activity Control, Chosun University)
  • 발행 : 2009.07.25
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초록

In this study, methotrexate (MTX)-encapsulated polymeric micelles using methoxy poly(ethylene glycol) (MPEG)-grafted chitosan (ChitoPEG) copolymer were prepared. The MIX-incorporated polymeric micelles of ChitoPEG copolymer has a particle size of around 50-100 nm. In 1H nuclear magnetic resonance (NMR) study, the specific peaks of MTX disappeared in heavy water ($D_2O$) and only the specific peak of MPEG was observed, while all of the peaks were confirmed in dimethyl sulfoxide (DMSO). These results indicated that MTX was complexed with chitosan and then formed an ion complex inner-core of the polymeric micelle in an aqueous environment. The drug contents of the polymeric micelle were around $4{\sim}12%$ and the loading efficiency of MTX in the polymeric micelles was higher than 60% (w/w) for all of the formulations. The cytotoxicity of MIX and MTX-incorporated polymeric micelle against CT26 tumor cells was not significantly changed.

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