The Cytotoxic Effect of 3,4,5-Trihydroxybenzoic Acid and Related Compounds against Oral Cancer Cell Lines

3,4,5-Trihydroxybenzoic Acid와 관련 화합물의 구강암 세포주에 대한 세포독성효과

  • Han, Du-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Lee, Jae-Sug (Department of Beauty Science, Kwangju Women's University) ;
  • Kang, Jeong-Il (Department of Physical Therapy, Daebul University Youngam) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional School of Oriental Medicine, Wonkwang University)
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 이재숙 (광주여자대학교 미용과학과) ;
  • 강정일 (대불대학교 물리치료학과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Received : 2009.07.22
  • Accepted : 2009.08.10
  • Published : 2009.08.30

Abstract

Objectives : In order to evaluate the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds on the growth of normal cell lines and human oral epithelioid cell line, cell viability, cell adhesion ability, and morphological changes of cells were examined. Methods : We measured the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds with 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide-[MTT), and 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium -5-caboxanilide-[XTT) methods. Results : The cytotoxicity of 3,4,5-trihydroxybenzoic acid($IC_{50}$, $2,552.40\;{\mu}M$) was low according to the toxic criteria. Cytotoxic effect of 3,4,5-trihydroxybenzoic acid and related compounds against $IC_{50}$ value in cell morphology increased in a concentration-dependent manner. In light microscopy, $100\;{\mu}M$ 3,4,5-trihydroxy-benzoic acid showed th highest cytotoxic activity. Conclusions : These results suggest that 3,4,5-trihydroxybenzoic acid may have a potential anticancer activity.

Keywords

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