Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
권호 표지

The Cytotoxic Effect of 3,4,5-Trihydroxybenzoic Acid and Related Compounds against Oral Cancer Cell Lines

3,4,5-Trihydroxybenzoic Acid와 관련 화합물의 구강암 세포주에 대한 세포독성효과

  • Han, Du-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Lee, Jae-Sug (Department of Beauty Science, Kwangju Women's University) ;
  • Kang, Jeong-Il (Department of Physical Therapy, Daebul University Youngam) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional School of Oriental Medicine, Wonkwang University)
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 이재숙 (광주여자대학교 미용과학과) ;
  • 강정일 (대불대학교 물리치료학과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Received : 2009.07.22
  • Accepted : 2009.08.10
  • Published : 2009.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : In order to evaluate the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds on the growth of normal cell lines and human oral epithelioid cell line, cell viability, cell adhesion ability, and morphological changes of cells were examined. Methods : We measured the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds with 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide-[MTT), and 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium -5-caboxanilide-[XTT) methods. Results : The cytotoxicity of 3,4,5-trihydroxybenzoic acid($IC_{50}$, $2,552.40\;{\mu}M$) was low according to the toxic criteria. Cytotoxic effect of 3,4,5-trihydroxybenzoic acid and related compounds against $IC_{50}$ value in cell morphology increased in a concentration-dependent manner. In light microscopy, $100\;{\mu}M$ 3,4,5-trihydroxy-benzoic acid showed th highest cytotoxic activity. Conclusions : These results suggest that 3,4,5-trihydroxybenzoic acid may have a potential anticancer activity.

Keywords

References

  1. Faber E: The multistep nature of cancer development. Cancer Res. 1984 ; 44 : 4217- 4223.
  2. Weinstein IB: The origin of human cancer ;molecular mechanisms of carcinogenesis and their implications for cancer prevention and treatment. Cancer Res. 1998 ; 48 : 4135-4139.
  3. Schlepper JR : Prevention, detection and diganosis of head and neck cancer. Semi Oncol. 1989 ; 5 : 139-149. https://doi.org/10.1016/0749-2081(89)90086-7
  4. Woods KV, Shillitoe EJ, Spitz MR, Shantz SP, Adler SK: Analysis of human papilloma virus DNA in oral squamous cell carcinomas. J Oral Pathol Med. 1993 ; 2 : 101-108.
  5. Slaughter DL, Southwick HW, Smejkaw W: Field cacerization in oral stratified squamous epithelium. clinical implication of muticentric origin. Cancer 1953 ; 6 : 963-968. https://doi.org/10.1002/1097-0142(195309)6:5<963::AID-CNCR2820060515>3.0.CO;2-Q
  6. Shin DM, Kim J, Ro JY, Hittelman J, Roth JA, Hong WK, Hittelman WN: Activation of p53 gene expression in premalignant lesions during head and neck tumorigenesis. Cancer Res. 1994 ; 54 : 321-326.
  7. Voravud N, Shin DM, Ro JY, Lee JS, Hong WK, Hittelman WN: Detection of chromosomes 7 and 17 aneuploidies during multistep carcinogenesis of head and neck cancer by in sit hybridization. Cancer Res. 1993 ; 53 : 2874-2883.
  8. Ray D, Sarma KD, Antony A: Differential effects of tri-n-butylstannyl benzoates on induction of apoptosis in K562 and MCF-7 cells. IUBMB Life 2000 ; 49 : 519-525. https://doi.org/10.1080/15216540050167061
  9. Lee HS: Tyrosinase inhibitors of Pulsatilla cernua root-derived materials. J Agric Food Chem. 2002 ; 50 : 1400-1403. https://doi.org/10.1021/jf011230f
  10. Ishiwata H, Nishijima M, Fukasawa Y: Estimation of preservative concentrations in foods and their daily intake based on official inspection results in Japan in fiscal year 1998. Shokuhin Eiseigaku Zasshi 2001 ; 42 : 404-412. https://doi.org/10.3358/shokueishi.42.404
  11. Reichelt M, Brown PD, Schneider B, Oldham NJ, Stauber E, Tokuhisa J, Kliebenstein DJ, Mitchell-Olds T, Gershenzon J : Benzoic acid glucosinolate esters and other glucosinolates from Arabidopsis thaliana. Phytochem. 2002 ; 59 : 663-671. https://doi.org/10.1016/S0031-9422(02)00014-6
  12. Wu TS, Chan YY, Leu YL: The constituents of the root and stem of Aristolochia cucurbitifolia Hayata and their biological activity. Chem PharmBull(Tokyo). 2000 ; 48 : 1006-1009. https://doi.org/10.1248/cpb.48.1006
  13. Kammerer D, Carle R, Schieber A : Characteriztion of phenolic acids in black carrots(Daucus carota ssp. sativus var. atrorubens Alef.) by high-performance liquid chromatography ionization mass spectrometry. Rapid Commun Spectrom. 2004 ; 18 : 1331-1340. https://doi.org/10.1002/rcm.1496
  14. Abd El-Mawla AA, Beerhues L : Benzoic acid biosynthesis in cell cultures of Hypericum androsaemum. Planta 2002 ; 214 : 727-733. https://doi.org/10.1007/s004250100657
  15. Wilhelm KP, Bottjer B, Siegers CP : Quantitative assessment of primary skin irritants in vitro in a cytotoxicity model : comparison with in vivo human irritation tests. Br J Dermatol. 2001 ; 145 : 709-715. https://doi.org/10.1046/j.1365-2133.2001.04497.x
  16. Nunez Selles AJ, Velez Castro HT, Aguero-Aguero J, Gonzalez-Gonzalez J, Naddeo F, De Simone F, Rastrelli L: Isolation and quantitative analysis of phenolic antioxidants, free sugars, and polyols from mango(Mangifera indica L.) stem bark aqueous decoction used in Cuba as a nutritional supplement. J Agric Food Chem. 2002 ; 50 : 762-766. https://doi.org/10.1021/jf011064b
  17. Murakami K, Matsuura T, Sano M, Hashimoto A, Yonekura K, Sakukawa R, Yamada Y, Saiki I: 4-[3,5-Bis(trimethylsilyl) benzamido] benzoic acid(TAC-101) inhibits the intrahepatic spread of hepatocellular carcinoma and prolongs the life-span of tumor-bearing animals. Clin Exp Metastasis 1998 ; 16 : 633-643. https://doi.org/10.1023/A:1006567229929
  18. De Graff WG, I. Myers SJr, Mitchell JB, Hahn SM: Protection against adriamycin cytotoxicity and inhibition of DNA topoisomerase II activity by 3,4-dihydroxybenzoic acid. Int J Oncol. 2003 ; 23 : 159-163.
  19. Costa-Lotufo LV, Jimenez PC, Wilke DV, Leal LK, Cunha GM, Silveira ER, Canuto KM, Viana GS, Moraes ME, de Moraes MO, Pessoa C: Anti- proliferative effects of several compoun GS,solated from Amburana cearensis A. C. Smith. Z Naturforsch [C] 2003 ; 58 : 675-680.
  20. Marom M, Haklai R, Ben-Baruch G, Marciano D, Egozi Y, Kloog Y: Selective inhibition of ras-dependent cell growth by farnesylthiosalisylic acid. J Biol Chem. 1995 ; 270 : 22263-22270. https://doi.org/10.1074/jbc.270.38.22263
  21. Abu-Amsha R, Croft KD, Puddey IB, Proudfoot JM, Beilin LJ : Phenolic content of various beverages determines the extent of inhibition of human serum and low-density lipoprotein oxidation in vitro: Identification and mechanism of action of some cinnamic acid derivatives from red wine. Clin Sci.(Colch.) 1996 ; 91 : 449-458.
  22. Rice-Evans CA, Miller NJ, Paganga G: Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996 ; 20 : 933-956. https://doi.org/10.1016/0891-5849(95)02227-9
  23. Han DS, Kang JL, Baek SH: Cytoprotective effect of phenolic compounds against hexavalent chromium-induced cytotoxicity. Kor. J. Orien. Prev. Med. Soc. Acceptd.
  24. Sauvant MP, Pepin D, Guillot J : Effects of humic substances and phenolic compounds on the in vitro toxicity of aluminium. Ecotoxicol Environ Saf. 1999 ; 44 : 47-55. https://doi.org/10.1006/eesa.1999.1799
  25. Mosmann T: Rapid colorimetric assays for cellular growth and survival : Application to proliferation and cytotoxicity assays. J Immunol Methods 1983 ; 65 : 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  26. 채영암, 구자옥, 서학수, 이영만: 기초생물 통계학 제 9장 직선회귀. 서울:학문사. 1991 ; pp.179-198.
  27. Valgimigli M, Merli Elgimlagutti P, Soukhomovskaia O, CicchitelliValgAntelliV A, Canistro D, FrancoliniValgimcriValg imstrorilli F, PaoliniVM, Gerrari R: HydroxcochidicaiVAeneration, levels of tumor necrosis factor-aipha, and progression to heart failure after acute myocardial in fmigtion. J Am Coll Cardiol. 2004 ; 43Pao1000-1008.
  28. Kalenilova EI, Gorodetskaya EA, Murashev AN, Ruuge EK, Medvedev OS : Role of reactive oxygen species in the sensitivity of rat hypertrophied myocardium to ischemia. Biochem.(Mosc) 2004 ; 69 : 311- 316. https://doi.org/10.1023/B:BIRY.0000022063.32185.7c
  29. Hashimoto T, Yonetani M, Nakamura H: Selective brain hypothermia protects against hypoxic-ischemic injury in newborn rats by reducing hydroxyl radical production. Kobe J Med Sci. 2003 ; 49 : 83-91.
  30. Han DS, Park YK, Kang JI, Baek SH: The cytotoxic effect of benzoic acid and related compounds against oral cancer cell lines. J Pharm Soc Korea 2008 ; 52 ; 252-258.
  31. Borenfreund E, Babichi H, Matin-Alcuacil N : Comparisons of two in vitro cytotoxicity assay. The neutral red(NR) and tetrazolium MTT tests. Toxicol In Vitro 1988 ; 2 : 1-8. https://doi.org/10.1016/0887-2333(88)90030-6