Journal of Ginseng Research

  • 제33권2호
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  • Pages.132-138
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  • 2009
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  • 1226-8453(pISSN)
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  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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방사선 조사 마우스에서 학습기억 장애에 대한 홍삼의 효과

Effect of Red Ginseng on Radiation-induced Learning and Memory Impairment in Mouse

  • 이해준 (한국원자력의학원) ;
  • 김중선 (한국원자력의학원) ;
  • 문창종 (전남대학교 수의과대학, 전남대학교 동물의학연구소) ;
  • 김종춘 (전남대학교 수의과대학, 전남대학교 동물의학연구소) ;
  • 조성기 (한국원자력연구원 정읍 방사선과학연구소) ;
  • 장종식 (경북대학교 축산학과) ;
  • 김성호 (전남대학교 수의과대학, 전남대학교 동물의학연구소)
  • Lee, Hae-June (Korea Institute of Radiological & Medical Science) ;
  • Kim, Joong-Sun (Korea Institute of Radiological & Medical Science) ;
  • Moon, Chang-Jong (College of Veterinary Medicine, Chonnam National University, Animal Medical Center, Chonnam National University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University, Animal Medical Center, Chonnam National University) ;
  • Jo, Sung-Kee (Advanced Radiation Technology Institute, KAERI) ;
  • Jang, Jong-Sik (Department of Animal Science, Kyungpook National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University, Animal Medical Center, Chonnam National University)
  • 발행 : 2009.06.30
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초록

Previous studies suggest that even low-dose irradiation can lead to progressive cognitive decline and memory deficits, which implicates, in part, hippocampal dysfunction in both humans and experimental animals. In this study, whether red ginseng (RG) could attenuate memory impairment was investigated through a passive-avoidance and object recognition memory test, as well as the suppression of hippocampal neurogenesis, using the TUNEL assay and immunohistochemical detection with markers of neurogenesis (Ki-67 and doublecortin (DCX)) in adult mice treated with a relatively low-dose exposure to gamma radiation (0.5 or 2.0 Gy). RG was administered intraperitonially at a dosage of 50 mg/kg of body weight, at 36 and 12 h pre-irradiation and at 30 minutes post-irradiation, or orally at a dosage of 250 mg! kg of body weight/day for seven days before autopsy. In the passive-avoidance and object recognition memory test, the mice that were trained for one day after acute irradiation (2 Gy) showed significant memory deficits compared with the sham controls. The number of TUNEL-positive apoptotic nuclei in the dentate gyrus (DG) was increased 12 h after irradiation. In addition, the number of Ki-67- and DCX-positive cells was significantly decreased. RG treatment prior to irradiation attenuated the memory defect and blocked apoptotic death as well as a decrease in the Ki-67- and DCX-positive cells. RG may attenuate memory defect in a relatively low-dose exposure to radiation in adult mice, possibly by inhibiting the detrimental effect of irradiation on hippocampal neurogenesis.

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참고문헌

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