The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory Effect of Bodusan

보두산(寶豆散) 메탄올 추출물의 항염증 효과

  • Kim, Pan-Joon (Department of Prescriptionology. Collage of Oriental Medicine, Dongguk University) ;
  • Yun, Hyun-Jeong (Cardiovascular Medical Research Center. Dongguk University) ;
  • Heo, Sook-Kyoung (Cardiovascular Medical Research Center. Dongguk University) ;
  • Kim, Kyoung-Ae (Department of Prescriptionology. Collage of Oriental Medicine, Dongguk University) ;
  • Kim, Dong-Wan (Department of Prescriptionology. Collage of Oriental Medicine, Dongguk University) ;
  • Kim, Jae-Eun (Department of Pathology, Collage of Oriental Medicine, Dongguk University) ;
  • Park, Sun-Dong (Cardiovascular Medical Research Center. Dongguk University, Department of Prescriptionology. Collage of Oriental Medicine, Dongguk University)
  • 김판준 (동국대학교 한의과대학 방제학교실) ;
  • 윤현정 (동국대학교 심혈관계질환 천연물 연구개발센터) ;
  • 허숙경 (동국대학교 심혈관계질환 천연물 연구개발센터) ;
  • 김경애 (동국대학교 한의과대학 방제학교실) ;
  • 김동완 (동국대학교 한의과대학 방제학교실) ;
  • 김재은 (동국대학교 한의과대학 병리학교실) ;
  • 박선동 (동국대학교 심혈관계질환 천연물 연구개발센터, 동국대학교 한의과대학 방제학교실)
  • Published : 2009.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Inflammation is important event in the development of vascular diseases including hypertension, atherosclerosis, and restenosis. Bodusan (BDS) was a traditional Korean herbal medicine and widely used in treatment of gastrointestinal complaint and stomach ulcer. The aim of this study was to determine whether BDS and its components inhibit production of nitrite, PGE2 and proinflammatory cytokines in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Methods : Cytotoxic activity of BDS and its components on RAW 264.7 cells was using 5-(3caroboxymcrophages. eth-oxyphenyj)-2H-tetra-zolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines were measured by ELISA kit. The levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were detected by western blot. Results : Our results indicated that BDS and its components significantly inhibited the LPS-induced NO and PGE2 production. Moreover. BDS and its components inhibited iNOS and COX-2 expression accompanied by an attenuation of TNF-${\alpha}$, IL-11${\beta}$, IL-6 and MCP-1 formation in macrophages. Conclusions: These results indicate that BDS and its components have potential as an anti-inflammatory agent.

Keywords

References

  1. 신길구. 신씨본초학. 서울 : 수문사. 1982 : 705.
  2. 김재길. 원색천연약물대사전 (상). 서울 : 남산당. 1984 : 215.
  3. Genevieve Philippe, Luc Angenot, Monique Tits, Michel Frederich. About the toxicity of some Strychnos species and their alkaloids. Toxicon. 2004 ; 44(1) : 405-16. https://doi.org/10.1016/j.toxicon.2004.05.006
  4. The Committee of the Pharmacopoeia of the Ministry of Health of the people's Republic of China. Pharmacopoeia of the People's Republic of China. vol 1. Beijing : Chemical Industry Press. 2000 : 38.
  5. Lee TH, Kwak HB, Kim HH, Lee ZH, Chung DK, Baek NI, Kim J. Methanol extracts of Stewartia koreana inhibit cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression by blocking NF-kappa B transactivation in LPS-activated RAW 264.7 cells. Mol Cells. 2007 ; 23(3) : 398-404,
  6. Nishida T, Yabe Y, Fu HY, Hayashi Y, Asahi K, Eguchi H, Tsuji S, Tsujii M, Hayashi N, Kawano S. Geranylgeranylacetone induces cyclooxygenase-2 expression in cultured rat gastric epithelial cells through NF-kappaB. Dig Dis Sci. 2007 ; 52(8) : 1890-6. https://doi.org/10.1007/s10620-006-9661-8
  7. 김태운, 조종관. 烏貝散과 寶豆의 병용투여가 위장관에 미치는 영향. 대한한방내과학회지. 1994 ; 15(2) : 27-36.
  8. 조유경, 김진성, 윤상협, 류봉하, 류기원. 寶豆의 修治法에 따른 毒性 및 消化器系에 미치는 영향. 대한한방내과학회지. 2002 ; 23(1) : 107-16.
  9. 윤현정, 서교수, 최재우, 이현우, 허숙경, 박원환, 박선동. 보두산(寶豆散)에 의한 SNU-1 세포의 Apoptosis 유도와 Cell cycle arrest. 대한본초학회지. 2007 ; 22(2) : 35-43.
  10. Ahmed MD, Alcock RA, Chendil D, Dey S, Das A, Venkatasubbarao K, Mohiuddin M, Sun L, Strodel WE, Freeman JW. Restoration of transforming growth factor-beta signaling enhances radiosensitivity by altering the Bcl-2/Bax ratio in the p53 mutant pancreatic cancer cell line MIA PaCa-2. J Biol Chem. 2002 ; 277(3) : 2234-46. https://doi.org/10.1074/jbc.M110168200
  11. Cheng EH, Wei MC, Weiler S, Flavell RA, Mak TW, Lindsten T, Korsmeyer SJ. BCL-2, BCL-X(L) sequester BH3 domain-only molecules preventing BAX- and BAK-mediated mitochondrial apoptosis. Mol Cell. 2001 ; 8(3) : 705-11. https://doi.org/10.1016/S1097-2765(01)00320-3
  12. Xu-Kun Deng, Wu-Yin, Wei-Dong Li, Fang-Zhou Yin, Xiao-Yu Lu, Xiao-Chun Zhang, Zi-Chun Hua, Bao-Chang Cai. The anti-tumor effects of alkaloids from the seeds of Strychnos nux-vomica on HepG2 cells and its possible mechanism. J Ethnopharmacol. 2006 ; 106 : 179-86. https://doi.org/10.1016/j.jep.2005.12.021
  13. Wu Yina, Xu-Kun Dengb, Fang-Zhou Yin, Xiao-Chun Zhang, Bao-Chang Cai. The cytotoxicity induced by brucine from the seed of Strychnos nux-vomica proceeds via apoptosis and is mediated by cyclooxygenase 2 and caspase 3 in SMMC 7221 cells. Food and Chemical Toxicol. 2007 ; 45 : 700-8.
  14. Desai A, Vyas T, Amiji M. Cytotoxicity and apoptosis enhancement in brain tumor cells upon coadministration of paclitaxel and ceramide in nanoemulsion formulations. J Pharm Sci. 2008 ; 97(7) : 2745-56. https://doi.org/10.1002/jps.21182
  15. Wang S, Chen Y, He D, He L, Yang Y, Chen J, Wang X. Inhibition of vascular smooth muscle cell proliferation by serum from rats treated orally with Gastrodia and Uncaria decoction, a traditional Chinese formulation. J Ethnopharmacol. 2007 ; 114(3) : 458-62. https://doi.org/10.1016/j.jep.2007.08.039
  16. Lee TH, Kwak HB, Kim HH, Lee ZH, Chung DK, Baek NI, Kim J. Methanol extracts of Stewartia koreana inhibit cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression by blocking NF-kappa B transactivation in LPS-activated RAW 264.7 cells. Mol Cells. 2007 ; 23(3) : 398-404.
  17. Nishida T, Yabe Y, Fu HY, Hayashi Y, Asahi K, Eguchi H, Tsuji S, Tsujii M, Hayashi N, Kawano S. Geranylgeranylacetone induces cyclooxygenase-2 expression in cultured rat gastric epithelial cells through NF-kappa B. Dig Dis Sci. 2007 ; 52(8) : 1890-6. https://doi.org/10.1007/s10620-006-9661-8
  18. Toda N, Nakanishi-Toda M. Nitric oxide: ocular blood flow, glaucoma, and diabetic retinopathy. Prog Retin Eye Res. 2007 ; 26(3) : 205-38. https://doi.org/10.1016/j.preteyeres.2007.01.004