Reproductive and Developmental Biology
- Volume 33 Issue 2
- /
- Pages.85-91
- /
- 2009
- /
- 1738-2432(pISSN)
- /
- 2288-0151(eISSN)
Effects of FBS(Fetal Bovine Serum) and pFF(Porcine Follicular Fluid) on In Vitro Maturation and Development of Porcine Parthenogenetic and Nuclear Transfer Embryos
- Moon, Hyo-Jin (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
- Shim, Joo-Hyun (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
- Hwang, In-Sun (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
- Park, Mi-Rung (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
- Kim, Dong-Hoon (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
- Ko, Yeoung-Gyu (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
-
Park, Choon-Keun
(College of Animal Science, Kangwon National University) ;
- Im, Gi-Sun (Division of Animal Biotechnology, National Institute of Animal Science, RDA)
- Received : 2009.06.11
- Accepted : 2009.06.18
- Published : 2009.06.30
Abstract
In this study, in vitro maturation system using fetal bovine serum (FBS) or porcine follicular fluid (pFF) was investigated to produce comparable oocytes to those derived from in vivo. Control group of oocytes was cultured in TCM 199 supplemented with 0.1% polyvinyl alcohol (PVA). Other three groups of oocytes were cultured in TCM 199 supplemented with 10% FBS, 10% pFF or 5% FBS + 5% pFF, respectively. After 44 h maturation, oocytes with the first polar body were activated with two electric pulses (DC) of 1.2 kv/cm for 30