Inhibition of Adipogenesis in 3T3-L1 Adipocytes with Magnolia officinalis Extracts

후박 추출물의 지방세포 분화 억제 효능에 관한 연구

  • Published : 2009.06.30


Magnolia extract, prepared from the Chinese herb Magnolia officinalis, is known for its potent anti-oxidative and anti-inflammatory effects. In this report, we showed that Magnolia extract inhibits adipocyte differentiation, as evidenced by reduced triglyceride (TG) accumulation. Also, Magnolia extract increased hormone sensitive lipase (HSL) protein level, and decreased the adipogenic transcription factor peroxisome proliferator activated receptor (PPAR)-${\gamma}$ protein and their corresponding mRNA. Our results suggest a potential apllication of Magnolia extract as anti-obesity agents inhibits adipocyte differentiation through the down-regulation of adipogenic transcription factors and other adipocyte-specific genes.

후박추출물은 중국 원산인 당후박에서 제조되며, 항산화 및 항염증 효과로 알려져 있다. 후박의 성분으로는 폴레페놀인 honokiol 및 magnolol 등이 알려져 있다. 이 연구에서, 우리는 후박추출물이 triglyceride (TG)의 축척을 감소시킴으로써 지방세포 분화를 억제한다는 표면적인 결과를 얻었다. 또한, 후박 추출물이 hormone sensitive lipase (HSL) 단백질 수준을 증가시키고 adipocyte에 specific한 transcription factors로 peroxisome proliferator activated receptor (PPAR)-${\gamma}$의 단백질과 mRNA 수준을 억제하였다. 결과적으로, 후박추출물이 항비만제로서 adipogenic transcription factor와 그들의 특이적인 유전자의 발현을 억제함으로써 지방세포 분화를 억제한다고 사료된다.



  1. P. B. Snyder, J. M. Esselstyn, K. Loughney, S. L. Wolda, and V. A. Florio, The role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysis, J. Lipid Res., 46, 494 (2005)
  2. D. S. Gray, Diagnosis and prevalence of obesity, Med. Clin. North Am., 73, 1 (1989)
  3. G. Ailhaud, P. Grimaldi, and R. Negrel, Cellular and aspects of adipose tissue development, Annu. Rev. Nutr., 12, 207 (1992)
  4. F. M. Gregoire, C. M. Smas, and H. S. Sul, Understanding adipocyte differentiation, Physiol. Rev., 78, 783 (1998)
  5. X. Guo and L. Kan, Analysis gene expression profile during 3T3-L1 preadipocyte differentiation, Gene., 251, 45 (2000)
  6. Z. Cao, R. M. Umek, and S. L. McKnight, Regulated expression of three C/EBP isoforms during aipose conversion of 3T3-L1 cells, Genes Dev., 5(9), 1538 (1991)
  7. W. C. Yeh, Z. Cao, M. Classon, and S. L. McKnight, Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP familly of leucine zipper proteins, Genes Dev., 9(2), 168 (1995)
  8. Q. Q. Tang, M. S. Jiang, and M. D. Lane, Repressive effect of Sp1 on the C/EBP$\alpha$gene promoter: role in adipocyte differentiation, Mol. Cell. Biol., 19(7), 4855 (1999)
  9. J. Y. Yang, M. A. Della Fera, S. Rayalam, and C. A. Baile, Enhanced effects of xanthohumol plus honokiol on apoptosis in 3T3-L1 adipocytes, Obesity, 16(6), 1232 (2008)
  10. S. W. Choi, C. S. Kim, M. S. Choi, B. H. Kim, H. S. Kim, and D. S. Choi, The study for antioxidative effects of Acanthopanax sessiliflorus extract as a new cosmetics ingredients using electron paramagnetic resonance, J. Soc. Cosmet. Scientists Korea, 31(1), 73 (2005)
  11. T. Mosmann, Rapid colorimetric assay for the cellular growth and survival: application to proliferation and cytotoxic assay, J. Immun. Methods, 65, 55 (1983)