Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Selective Sterilization of Vibro parahaemolyticus from a Bacterial Mixture by Low-Amperage Electric Current

  • Jin, Soo-Chang (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Yoo, Hyun-Suk (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Woo, Yeon-I. (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Lee, Mi-Hee (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Vagaska, Barbora (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Kim, Jung-Sung (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Uzawa, Masakazu (Applied Science Co. Ltd.) ;
  • Park, Jong-Chul (Department of Medical Engineering, Yonsei University College of Medicine)
  • Published : 2009.06.30
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Abstract

The objective of this study was to investigate the possibility of using low-amperage electrical treatment (LAET) as a selective bacteriocide. Mixtures containing Escherichia coli, Staphylococcus aureus, and Vibrio parahaemolyticus were treated with different electric current intensities and for different times. The results showed that at 263 mA, treating bacteria for 100 ms eliminated all V. parahaemolyticus colonies. Although LAET reduced the populations of the three microorganisms, V. parahaemolyticus was more injured by LAET than S. aureus and E. coli when treated at the same processing conditions.

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References

  1. Dimitrov, D. S. and R. K. Jain. 1984. Membrane stability. Biochim. Biophys. Acta 779: 437-468
  2. Garcia, D., N. Gomez, P. Manas, S. Condon, J. Raso, and R. Pagan. 2005. Occurrence of sublethal injury after pulsed electric fields depending on the micro-organism, the treatment medium pH and the intensity of the treatment investigated. J. Appl. Microbiol. 99: 94-104 https://doi.org/10.1111/j.1365-2672.2005.02611.x
  3. Guillou, S. and N. El Murr. 2002. Inactivation of Saccharomyces cerevisiae in solution by low-amperage electric treatment. J. Appl. Microbiol. 92: 860-865 https://doi.org/10.1046/j.1365-2672.2002.01593.x
  4. Hatha, A. A. and P. Lakshmanaperumalsamy. 1995. Antibiotic resistance of Salmonella strains isolated from fish and crustaceans. Lett. Appl. Microbiol. 21: 47-49 https://doi.org/10.1111/j.1472-765X.1995.tb01004.x
  5. Kim, S.-Y., C.-H. Lee, K.-J. Kim, and Y.-S. Kim. 2001. Expression of the functional recombinant interleukin-16 in E. coli and mammalian cell lines. J. Microbiol. Biotechnol. 11:234-241
  6. Lvarez, A., R. Pagan, J. Raso, and S. Condon. 2002. Environmental factors influencing the inactivation of Listeria monocytogenes by pulsed electric fields. Lett. Appl. Microbiol. 35: 489-493 https://doi.org/10.1046/j.1472-765X.2002.01221.x
  7. Mann, A., M. Kiefer, and H. Leuenberger. 2001. Thermal sterilization of heat-sensitive products using high-temperature short-time sterilization. J. Pharm. Sci. 90: 275-287 https://doi.org/10.1002/1520-6017(200103)90:3<275::AID-JPS3>3.0.CO;2-I
  8. Miller, C. M. and R. L. Valentine. 1995. Oxidation behavior of aqueous contaminants in the presence of hydrogen peroxide and filter media. J. Hazard. Mater. 41: 105-116 https://doi.org/10.1016/0304-3894(94)00098-2
  9. Mosqueda-Melgar, J., R. M. Raybaudi-Massilia, and O. Martín- Belloso. 2007. Influence of treatment time and pulse frequency on Salmonella enteritidis, Escherichia coli and Listeria monocytogenes populations inoculated in melon and watermelon juices treated by pulsed electric fields. J. Food Microbiol. 117:192-200 https://doi.org/10.1016/j.ijfoodmicro.2007.04.009
  10. Park, J.-C., M. S. Lee, D. W. Han, D. H. Lee, B. J. Park, I.-S. Lee, M. Uzawa, M. Aihara, and K. Takatori. 2004. Inactivation of bacteria in seawater by low-amperage electric current. Appl. Environ. Microbiol. 70: 2405-2408
  11. Smith, R. J., S. C. Kehoe, K. G. McGuigan, and M. R. Barerl. 2000. Effect of simulated solar disinfection of water on infectivity of Salmonella typhimurium. Lett. Appl. Microbiol. 31: 284-288 https://doi.org/10.1046/j.1472-765x.2000.00815.x
  12. Weaver, J. C. 1995. Electroporation theory: concepts and mechanisms, pp. 1-26. In Nickoloff, J. A. (ed.), Electroporation Protocols for Microorganisms. Humana Press, Totowa, New Jersey