Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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HPLC Analysis and Screening of Standard Compound on Saposhnikoviae Radix for Standardization of GCSB-5 Preparation

생약복합제 GCSB-5의 품질 표준화를 위한 방풍의 지표성분 탐색 및 HPLC 분석

  • Cha, Bae-Cheon (Department of Pharmaceutical Engineering, College of Health Sciences, Sangji University) ;
  • Lee, Eun-Hee (Department of Pharmaceutical Engineering, College of Health Sciences, Sangji University)
  • 차배천 (상지대학교 보건과학대학 제약공학과) ;
  • 이은희 (상지대학교 보건과학대학 제약공학과)
  • Published : 2009.06.30
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Abstract

GCSB-5 preparation is a purified extract from a mixture of 6 medicinal plants(Acanthopanacis Cortex, Achyranthis Radix, Saposhnikoviae Radix, Cibotii Rhizoma, Glycine Semen Nigra, Eucommiae Cortex) that have been widely used for the treatment of various bone disorders. The aim of this study was to investigate HPLC analysis method and screening of standard compound on Saposhnikoviae Radix for quality standardization of a medicinal crude drug GCSB-5. Standard compound of Saposhnikoviae Radix was decided with cimifugin by isolation and instrumental analysis such as NMR. HPLC analysis method for the simultaneous determination of cimifugin was established for the quality control of the medicinal plants of Saposhnikoviae Radix species, GCSB-5 raw material and preparation. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline.

Keywords

References

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