Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
권호 표지

Antioxidant Effects of Gagam-jangwon-hwan(jiajianzhuangyuanwan) on Hydrogen Peroxide-Induced Oxidative Stress in PC 12 Cell Lines

$H_2O_2$로 유도된 산화적 스트레스에 대한 장원환가감방(壯元丸加減方)의 PC 12 cell 에서의 항산화 효과

  • Park, Yong-Hoon (IN AM NeuroScience Research Center, Sanbon Medical Center, Wonkwang University) ;
  • Son, Il-Hong (IN AM NeuroScience Research Center, Sanbon Medical Center, Wonkwang University) ;
  • Lee, Sang-Won (National Institute of Horticultural and Herbal Science) ;
  • Lim, Jung-Hyun (Dept. of neuropsychiatry, college of Oriental Medicine, Wonkwang University) ;
  • Kim, Tae-Heon (Dept. of neuropsychiatry, college of Oriental Medicine, Wonkwang University) ;
  • Lyu, Yeoung-Su (Dept. of neuropsychiatry, college of Oriental Medicine, Wonkwang University) ;
  • Kang, Hyung-Won (Dept. of neuropsychiatry, college of Oriental Medicine, Wonkwang University)
  • 박용훈 (원광대학교 산본병원 인암뇌신경센타) ;
  • 손일홍 (원광대학교 산본병원 인암뇌신경센타) ;
  • 이상원 (국립원예특작과학원) ;
  • 임정현 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 김태헌 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 류영수 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 강형원 (원광대학교 한의과대학 한방신경정신과학교실)
  • Published : 2009.06.30
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Abstract

Objectives : Antioxidant effects of Gagam-jangwonhwan(LMK01 and 02) water extract against $H_2O_2$-induced oxidative damage and cell death were investigated in rat pheochromocytoma line PC 12. Methods : The cells were treated with LMK01 and 02 water extract and $H_2O_2$, oxidative damage-inducing materials for 24 h. The cellular viability was assessed by WST-1 assay, oxidative damages of the cells by 8-OHdG quantitation, apoptosis by Hoechst 33342 staining assay and activity of antioxidant enzymes by catalase and glutathione peroxidase assay. Results : 1. LMK01 and LMK02 water extracts improved significantly cell viability in $H_2O_2$-treated groups than $H_2O_2$-alone treated cells 2, LMK02 suppressed significantly oxidative damage in $H_2O_2$-treated groups than $H_2O_2$-alone treated cells but LMK01 didn't. Meanwhile, difference of oxidative damages in conditions treated with LMK01 or LMK02 was not significant, 3. The $H_2O_2$ induced-apoptosis in PC 12 cell lines was inhibited effectively by LMK01 and LMK02, and especially the features of apoptosis were obviously reduced in LMK02-treated cells. 4. LMK01 and LMK02 increased significantly activities of both catalase and glutathione peroxidase than those of $H_2O_2$-alone treated group and moreover, LMK02 showed significantly higher activities than those of LMK01. Conclusions : As shown, LMK01 and LMK02 suppressed $H_2O_2$-induced oxidative damage and cell death in PC 12 cell effectively. And they increased activity of major antioxidant enzymes in PC 12 cell line. Therefore, this study suggests the possibility of clinical usage over oxidative stress-induced neurodegenerative disease such as Alzheimer's disease.

Keywords

References

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