생명과학회지 (Journal of Life Science)

  • 제19권6호
  • /
  • Pages.787-792
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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제주 연안 퇴적층에서 분리된 미생물의 동정 및 단백질분해효소 특성

Identification of Microorganisms Isolated from Jeju Coastal Sedimentary Layer and Characterization of Their Proteases.

  • 김만철 (제주대학교 수산생명의학과) ;
  • 장태원 (제주대학교 수산생명의학과) ;
  • 김주상 (제주대학교 수산생명의학과) ;
  • 한용재 (제주대학교 수산생명의학과) ;
  • ;
  • 한송헌 (제주대학교 수산생명의학과) ;
  • 오덕철 (제주대학교 생명과학과) ;
  • 허문수 (제주대학교 수산생명의학과)
  • Kim, Man-Chul (Department of Aquatic Life Medicine, Jeju National University) ;
  • Jang, Tae-Won (Department of Aquatic Life Medicine, Jeju National University) ;
  • Kim, Ju-Sang (Department of Aquatic Life Medicine, Jeju National University) ;
  • Han, Yong-Jae (Department of Aquatic Life Medicine, Jeju National University) ;
  • Harikrishnan, Ramasamy (Department of Aquatic Life Medicine, Jeju National University) ;
  • Han, Song-Hun (Department of Aquatic Life Medicine, Jeju National University) ;
  • Oh, Duck-Chul (Department of Life Science, Jeju National University) ;
  • Heo, Moon-Soo (Department of Aquatic Life Medicine, Jeju National University)
  • 발행 : 2009.06.30
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초록

제주도 양식장 배출수 퇴적층에서 단백질 분해 효소를 생산하는 세균을 분리하였으며, 각각 SK-2, 및 SK-125라고 명명하였다. 분리균주 SK-2의 16S rDNA의 염기서열 분석 결과 Baeillus lieheniformis와 Baeillus subtillis의 염기서열과 99%의 상동성을 보였으며, BIOLOG를 이용한 생화학적 분석에서도 Bacillus family와 유사한 특성을 보여 최종적으로 분리균주 SK-2를 Baeillus sp. SK-2으로 명명하였다. 또한 분리균주 SK-125의 16S rDNA의 염기서열 분석 결과 Pseudoal teromonas haloplanktis의 염기서열과 99% 의 상동성을 보였으며, BIOLOG를 이용한 생화학적 분석에서도 Pseudoalteromonas family와 유사한 특성을 보여 최종적으로 분리균주 SK-125를 Pseudoalteromonas sp. SK-125라고 명명하였다. Baeillus sp. SK-2와 Pseudoalteromonas sp. SK-125균주의 온도별 실험에서는 $40^{\circ}C$에서 가장 높은 생육도를 보였으며, 효소활성은 Bacillus sp. SK-2와 Pseudoalteromonas sp. SK-125 두 균주 모두 $30^{\circ}C$에서 가장 높은 활성을 보였다.

In this study, protease-producing bacteria were isolated from the marine sedimentary layer in coastal Jeju. We isolated 2 protease producing strains (SK-2 and SK-125) and tested their protesase producing activities. Gram staining and BIOLOG of isolated strains revealed that strains SK-2 and SK-125 belong to Bacillus and Pseudoalteromonas families, respectively. The 16S rDNA nucleotide sequences analyses of the isolated strains showed 99% sequence homology with those of Bacillus sp. and Pseudoalteromonas sp.; therefore, the isolated strains SK-2 and SK-125 were named Bacillus sp. SK-2 and Pseudoalteromonas sp. SK-125, respectively. The optimum conditions for the cell growth of protease activities were obtained when the both isolates were cultured at $30^{\circ}C$, 96 hrs and pH $7{\sim}8$.

키워드

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