생명과학회지 (Journal of Life Science)

  • 제19권6호
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  • Pages.756-764
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

황금(黃芩), 석창포(石菖蒲), 천마(天麻) 물추출액 혼합물이 배양한 흰쥐 대뇌신경세포의 활성과 학습능력 증진 효능

Effects of Water-extract Mixture of Scutellariae baicalensis GEORGI, Acarus gramineus SOLAND and Gastrodia elata BLUME on Cultured Rat Cortical Neurons and Enhancement of Learning and Memory Power

  • 배철환 (동국대학교 한의과대학 내과학 교실) ;
  • 정현정 (동국대학교 한의과대학 내과학 교실) ;
  • 정승현 (동국대학교 한의과대학 내과학 교실) ;
  • 문일수 (동국대학교 의과대학 해부학교실) ;
  • 이원철 (부산대학교 한의학전문대학원) ;
  • 신길조 (동국대학교 한의과대학 내과학 교실)
  • 발행 : 2009.06.30
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초록

Scutellariae baicalensis GEORGt Acorus gramineus SOLAND and Gastrodia elata BLUME are traditional medicines used in the treatment of incipient stoke. In this study we investigated their effects on various aspects of neuronal differentiation in single or composite forms. Water-extracts of these medicines showed neuroprotective effects on cultured rat cortical neurons in normoxia and hypoxia. To understand the mechanism for neuroprotection we carried out various cell biological assays. They stimulated initial differentiation of neuronal development (transition from stage 1 to 2), and increased the number of spines and the length and number of dendritic processes. These effects were best manifested in the experimental group, which were given a mixture of the three kinds of extracts (p<0.01). To assess improvement of brain functions we carried out Morris water-maze tests for the mice that were fed on these extracts instead of water for 4 weeks. The experimental groups, especially those which were given the mixture of the three kinds of extract, showed significant (p<0.01) enhancement in memory as early as one day after the learning trial. These results indicate that these three kinds of extracts have synergistic effects on neuronal protection and improvement of brain functions.

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