동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제23권3호
  • /
  • Pages.631-638
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  • 2009
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

GLEDITSIAE SPINA 에탄올 추출물 및 분획물이 산화적 스트레스와 human LDL 산화억제에 미치는 영향

Preventive Effects of GLEDITSIAE SPINA Ethanol Extracts and its Fraction on Oxidative Stress and Human LDL Oxidation

  • 김혁 (동국대학교 한의과대학 기초의과학연구센터 & 진단학교실) ;
  • 이민자 (동국대학교 한의과대학 기초의과학연구센터 & 진단학교실) ;
  • 이혜숙 (동국대학교 한의과대학 기초의과학연구센터 & 진단학교실) ;
  • 정현정 (동국대학교 한의과대학 기초의과학연구센터 & 진단학교실) ;
  • 최성규 (동국대학교 한의과대학 기초의과학연구센터 & 진단학교실) ;
  • 이창섭 (동국대학교 한의과대학 기초의과학연구센터 & 진단학교실) ;
  • 박원환 (동국대학교 한의과대학 기초의과학연구센터 & 진단학교실)
  • Kim, Hyuck (Cardiovascular Medical Research Center and Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Lee, Min-Ja (Cardiovascular Medical Research Center and Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Lee, Hye-Sook (Cardiovascular Medical Research Center and Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Jung, Hyun-Jung (Cardiovascular Medical Research Center and Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Choi, Sung-Kyu (Cardiovascular Medical Research Center and Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Lee, Chang-Sub (Cardiovascular Medical Research Center and Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Park, Won-Hwan (Cardiovascular Medical Research Center and Department of Diagnostics, College of Korean Medicine, Dongguk University)
  • 발행 : 2009.06.25
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초록

GLEDITSIAE SPINA (GS) has been used as folk remedies traditionally for treatment of antiphlogistic and antifebrile agents. An ethanol extract and its fraction of GS were assessed to determine the mechanism of its antioxidant activity. Also, inhibitory effect of extract from GS and its fraction measuring the inhibitory effect on $Cu^{2+}$-induced human low-density lipoprotein (LDL) oxidation. GS ethanol extract and its fraction exhibited a concentration-dependent reactive oxygen species (ROS) and reactive nitrogen species (RNS) scavenging activities, including trolox equivalent antioxidant capacity (TEAC), OPPH radical, superoxide anion, hydroxyl radicals, peroxynitrite and nitric oxide, using different assay systems. Furthermore, the GS ethanol extract and its fraction showed dose-dependent protection of LDL oxidation induced by $CuSO_4$. In addition, the GS ethanol extract and its fraction were characterized as containing a high amount of total phenolics. These results suggest that GS ethanol extract and its fraction might be helpful for preventing oxidative stress and protecting LDL oxidation.

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