Effect of the Ethanol Extract of Artemisiae Capillaris Herba on the Hyperpigmentation Induced by ${\alpha}$-MSH

인진(茵蔯) 에탄올추출물이 ${\alpha}$-MSH로 유도된 과색소 형성에 미치는 영향

  • Shin, Ki-Don (Department of Herbology, College of Oriental Medicine, Sangji University) ;
  • Kim, Dae-Sung (Department of Herbal Resources. Professional Graduate School of Oriental Medicine, WonkWang University) ;
  • Lee, Jang-Cheon (Division of Pharmacology, School of Oriental Medicine, Pusan National University) ;
  • Mun, Yeun-Ja (Department of Anatomy, College of Oriental Medicine, WonkWang University) ;
  • Woo, Won-Hong (Department of Herbal Resources. Professional Graduate School of Oriental Medicine, WonkWang University) ;
  • Lee, Young-Cheal (Department of Herbology, College of Oriental Medicine, Sangji University)
  • 신기돈 (상지대학교 한의과대학 본초학교실) ;
  • 김대성 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이장천 (부산대학교 한의학전문대학원 약물의학부) ;
  • 문연자 (원광대학교 한의과대학 해부학교실) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이영철 (상지대학교 한의과대학 본초학교실)
  • Published : 2009.06.25

Abstract

Melanogenesis is induced mainly by ultraviolet radiation of sunlight and ${\alpha}$-Melanocyte stimulation hormone (${\alpha}$-MSH) which binds to a specific G protein coupled receptor. ${\alpha}$-MSH and cAMP-elevating agents are known to melanin syntheisis and dendrite outgrowth. The purpose of this study was to investigate the mechanism of melanogenesis inhibition in B16/F10 cells by ethanol extract of Artemisiae Capillaris Herba. In the present study, ${\alpha}$-MSH led to a stimulation of melanin synthesis that appeared to result from an increased tyrosinase activity and melanin content. However, the ethanol extract of Artemisiae Capillaris Herba inhibited the ${\alpha}$-MSH-induced tyrosinase activity and melanin content. In control conditions, B16/F10 cells displayed a fibroblastic appearance while ${\alpha}$-MSH treatment promoted the emergence of small and numerous dendrites from the plasma membrane. The ethanol extract of Artemisiae Capillaris Herba abolished the ${\alpha}$-MSH-induced dendricity. Regarding protein levels of the melanogenic enzymes, the amounts of tyrosinase were increased after incubation with ${\alpha}$-MSH. The treatment of Artemisiae Capillaris Herba ethanol extract decreased the ${\alpha}$-MSH expression levels of tyrosinase. Based on these findings, it is likely that the ethanol extract of Artemisiae Capillaris Herba exerts its depigmenting effects in B16/F10 cells through the suppression of tyrosinase expression, which are key enzymes for melanogenesis.

Keywords

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