Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
권호 표지

Changes in Differentially Expressed Genes in the Liver of Oryzias latipes by Binary Exposure to Carcinogenic Polycyclic Aromatic Hydrocarbons

  • Oh, Jeong-Hwan (Department of Biological Sciences, Pusan National University) ;
  • Moon, Hyo-Bang (Marine Environment Research Team, National Fisheries Research and Development Institute) ;
  • Choe, Eun-Sang (Department of Biological Sciences, Pusan National University)
  • Received : 2009.11.05
  • Accepted : 2009.11.25
  • Published : 2009.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

The biological effects of carcinogenic polycyclic aromatic hydrocarbons (cPAHs) including benzo[a]pyrene (BaP), dibenzo[a,h]anthracene (DBA), benzo[a]anthracene (BaA), benzo[b] fluoranthene (BbF), benzo[k]fluoranthene (BkF), and indeno[1,2,3-c, d]pyrene (InP) on transcriptomic changes were determined in the liver of Oryzias latipes. Differentially expressed genes (DEGs) by binary exposure to cPAHs (BaP+BaA, BaP+BbF, BaP+BkF, BaP+DbA, BaP+InP) were screened by annealing control primers-based polymerase chain reaction followed by sequence analysis and BLAST searching. The results showed that four DEGs were commonly expressed by cPAHs and they were identified as ribosomal protein S4, coagulation factor II, elongation factor 1 beta, and a predicted protein similar to human immunodeficiency virus type I enhancer binding protein 3. This finding suggests that binary exposure to cPAHs interferes protein synthesis required for fundamental liver functions in fish.

Keywords

References

  1. Adamo RD, S Pelosi, P Trotta and G Sansone. 1997. Bioaccumulation and biomagnification of polycyclic aromatic hydrocarbons in aquatic organisms. Mar. Chem. 56:45 https://doi.org/10.1016/S0304-4203(96)00042-4
  2. Aravindakshan J, V Paquet, M Gregory, J Dufresne, M Fournier, DJ Marcogliese and DG Cyr. 2004. Consequences of xenoestrogen exposure on male reproductive function in spottail shiners (Notropis hudsonius). Toxicol. Sci. 78:156 https://doi.org/10.1093/toxsci/kfh042
  3. Baumard P, H Budzinski, P Garrigues, JC Sorbe, T Burgeot and J Bellocq. 1998. Concentrations of PAHs (Polycyclic aromatic hydrocarbons) in various marine organisms in relation to those in sediments and to trophic level. Mar. Pollut. Bull. 36:951 https://doi.org/10.1016/S0025-326X(98)00088-5
  4. Carr-schmid A, L Valente, VI Loik, T Williams, LM Starita and TG Kinzy. 1999. Mutations in elongation factor 1, a guanine nucleotide exchange factor, enhance translational fidelity. Mol. Cell. Biol. 19:5257
  5. Carlson EA, Y Li and JT Zelikoff . 2004. Benzo[a]pyrene-induced immunotoxicity in Japanese medaka (Oryzias latipes): relationship between lymphoid CYP1A activity and humoral immune suppression. Toxicol. Appl. Pharmacol. 201:40 https://doi.org/10.1016/j.taap.2004.04.018
  6. Coughlin SR, TK Vu, DT Hung and VI Wheaton. 1992. Characterization of a functional thrombin receptor. Issues and opportunities. J. Clin. Invest. 89:351 https://doi.org/10.1172/JCI115592
  7. Fielden MR and TR Zacharewski. 2001. Challenges and limitations of gene expression profiling in mechanistic and predictive toxicology. Toxicol. Sci. 60:6 https://doi.org/10.1093/toxsci/60.1.6
  8. Gravato C and MA Santos. 2002. Juvenile sea bass liver P450, EROD induction and erythrocytic genotoxic responses to PAH and PAH-like compounds. Ecotoxicol. Environ. Saf. 51:115 https://doi.org/10.1006/eesa.2001.2133
  9. Greytak SR, D Champlin and GV Callard. 2005. Isolation and characterization of two cytochrome P450 aromatase forms in killifish (Fundulus heteroclitus): Differential expression in fish from polluted and unpolluted environments. Aquat. Toxicol. 71:371 https://doi.org/10.1016/j.aquatox.2004.12.007
  10. Guillette JLJ, DA Crain, AA Ronney and DB Pickford. 1995. Organization versus activation: The role of endocrine disrupting contaminants (EDCs) during embryonic development in wildlife. Environ. Health Perspect. 103:157
  11. Hiraga K, K Suzuki, E Tsuchiya and T Miyakawa. 1993. Cloning and characterization of the elongation factor EF-1b homologue of Saccharomyces cerevisiae. EF-1b is essential for growth. FEBS Lett. 316:165 https://doi.org/10.1016/0014-5793(93)81208-H
  12. Hoffmann JL and JT Oris. 2006. Altered gene expression: A mechanism for reproductive toxicity in zebrafish exposed to benzo(a)pyrene. Aquat. Toxicol. 78:332 https://doi.org/10.1016/j.aquatox.2006.04.007
  13. Hwang IT, YJ Kim, SH Kim, CI Kwak, YY Gu and JY Chun. 2003. Annealing control primer system for improving specificity of PCR amplification. BioTechniques 35:1
  14. Hylland K. 2006. Polycyclic aromatic hydrocarbon (PAH) ecotoxicology in marine ecosystems. J. Toxicol. Environ. Health Part A 69:109 https://doi.org/10.1080/15287390500259327
  15. IARC. 1987. IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans. Overall evaluation of carcinogenicity: an updating of IAPC monographs. vol. 1-42. Suppl. 7. International Agency for Research on Cancer, Lyon, France
  16. Inoue K and Y Takei. 2003. Asian medaka fishes offer new models for studying mechanisms of seawater adaptation. Comp. Biochem. Physiol. B 136:635 https://doi.org/10.1016/S1096-4959(03)00204-5
  17. Jonsson EM, A Abrahamson, B Brunstorm and I Brandt. 2006. Cytochrome P4501A induction in rainbow trout gills and liver following exposure to waterborne indigo, benzo(a) pyrene and 3,$3^{\prime}$,4,$4^{\prime}$,5-pentachlorobiphenyl. Aquat. Toxicol. 79:226 https://doi.org/10.1016/j.aquatox.2006.06.006
  18. Kapp LD and JR Lorsch. 2004. The molecular mechanics of eukaryotic translation. Annu. Rev. Biochem. 73:657 https://doi.org/10.1146/annurev.biochem.73.030403.080419
  19. Kim YJ, CI Kwak, YY Gu, IT Hwang and JY Chun. 2004. Annealing control primer system for identification of differentially expressed genes on agarose gels. BioTechniques 36:1
  20. Liebman SW, YO Chernoff and R Liu. 1995. The accuracy center of a eukaryotic ribosome. Biochem. Cell Biol. 73:1141 https://doi.org/10.1139/o95-123
  21. Lodish H, A Berk, P Matsudaira, CA Kaiser, M Krieger, MP Scott, SL Zipursky and J Darnell. 2003. Molecular cell biology. 5th ed. pp. 119-130. W.H. Freeman and Company, New York
  22. Moggs JG. 2005. Molecular responses to xenoestrogens: Mechanistic insights from toxicogenomics. Toxicology 213:177 https://doi.org/10.1016/j.tox.2005.05.020
  23. Nacci DE, M Kohan, M Pelletier and E George. 2002. Effects of benzo(a)pyrene exposure on a fish population resistant to the toxic effects of dioxin-like compounds. Aquat. Toxicol. 57:203 https://doi.org/10.1016/S0166-445X(01)00196-5
  24. Patel MR, BE Scheffler, L Wang and KL Willett. 2006. Effects of benzo(a)pyrene exposure on killifish (Fundulus heteroclitus) aromatase activities and mRNA. Aquat. Toxicol. 77:267 https://doi.org/10.1016/j.aquatox.2005.12.009
  25. Peters LD, HR Morse, R Waters and DR Livingstone. 1997. Responses of hepatic cytochrome P4501A and formation of DNAadducts in juveniles of turbot (Scophthalmus maximus L.) exposed to water-borne benzo[a]pyrene. Aquat. Toxicol. 38:67 https://doi.org/10.1016/S0166-445X(96)00838-7
  26. Riis B, SIS Rattan, BFC Clark and WC Merrick. 1990. Eukaryotic protein elongation factors. Trends Biol. Chem. 15:420 https://doi.org/10.1016/0968-0004(90)90279-K
  27. Sakamoto T, T Kozaka, A Takahashi, H Kawauchi and M Ando. 2001. Medaka (Oryzias latipes) as a model for hypoosmoregulation of euryhaline fishes. Aquaculture 193:347 https://doi.org/10.1016/S0044-8486(00)00471-3
  28. Sun WY, DP Witte, JL Degen, MC Colbert, MC Burkart, K Holmb$\ddot{a}$ck, Q Xiao, TH Bugge and SJ Degen. 1998 Prothrombin deficiency results in embryonic and neonatal lethality in mice. 1: Proc. Natl. Acad. Sci. U.S.A. 95:7597 https://doi.org/10.1073/pnas.95.13.7597
  29. Waring JF, R Ciurlionis, RA Jolly, M Heindel and RG Ulrich. 2001. Microarray analysis of hepatotoxins in vitro reveals a correlation between gene expression profiles and machanisms of toxicity. Toxicol. Lett. 120:359 https://doi.org/10.1016/S0378-4274(01)00267-3
  30. Williams TD, AM Diab, SG George, V Sabine and JK Chipman. 2007. Gene expression responses of European flounder (Platichthys flesus) to 17-beta estradiol. Toxicol. Lett. 168:236 https://doi.org/10.1016/j.toxlet.2006.10.020