Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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General Enzymatic Properties of Human Histidine Acid Phosphatase-Phytase

히스티딘 에시드 포스파테이즈(Histidine Acid Phosphatase) 계열 인간 파이테이즈(Phytase)의 일반적 특성규명

  • Cho, Jaie-Soon (Department of Animal Sciences and Environment, College of Animal Bioscience & Technology, Konkuk University)
  • 조재순 (건국대학교 동물생명과학대학 동물생산환경학)
  • Received : 2009.03.11
  • Accepted : 2009.04.20
  • Published : 2009.04.01
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Abstract

The glycosylated human MINPP (multiple inositol polyphosphate phosphatase), which was recombinantly over-expressed by using industrial host, Pichia pastoris, showed the phytase activity against phytate ($InsP_6$) and the enzyme activity of the unglycosylated counterpart was decreased to 30%. The optimal phytase activity occurred at pH 7.4. The human MINPP showed high substrate specificity for $InsP_6$ with little activity on other organic phosphate conjugates such as para-nitrophenylphosphate (pNPP), ATP, and ribose-1-phosphate (R-1-P). The phosphatase activity against 2,3-bisphosphoglycerate (2,3-BPG) by human MINPP was increased to 1.2-fold in the presence of stimulator, 1 mM 2-phosphoglycolate (2-PG) but the phytase activity against $InsP_6$ was not affected by addition of 1 mM 2-PG. The phosphatase activity against 2,3-BPG by human MINPP was not increased in the presence of 2 mM $Mg^{2+}$ or 100 mM $Cl^-$.

산업용 균주인 Pichia pastoris에서 재조합 발현된 당화된 인간유래 MINPP (multiple inositol polyphosphate phosphatase) 효소는 기질 피틴태인(InsP6)에 대한 파이테이즈 효소활성을 나타내었고 탈당화시 그 효소활성이 30% 감소되었다. 그 효소의 기질 $InsP_6$에 대한 최적 pH 활성은 중성범위인 pH 7.4였다. 기질특이성 측면에서 인간 MINPP 효소는 para-nitrophenylphosphate (pNPP), ATP, ribose-1-phosphate (R-1-P)와 같은 유기인산화합물(organic phosphate conjugates)의 분해활성은 매우 낮은 대신, 기질 $InsP_6$를 효과적으로 분해하였고 특히 화학적 자극제인(chemical stimulator)인 1 mM 2-phosphoglycolate (2-PG)의 첨가에 따른 기질 2,3-bisphosphoglycerate (2,3-BPG)에 대한 효소활성이 2-PG를 첨가하지 않을때 보다 1.2배 증가되었지만 기질 $InsP_6$에 대한 효소활성에는 영향을 주지 않았다. 또한 2 mM $Mg^{2+}$ 이온과 100 mM $Cl^-$ 이온의 첨가는 MINPP 효소의 기질 2,3-BPG에 대한 효소활성에 역시 영향을 주지 않았다.

Keywords

References

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