Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Association Study Between Polymorphisms of Inositol 1,4,5-triphosphate Receptor Type 1 (IP3R1) Gene and Carcass Traits in Korean Cattle (Hanwoo)

한우 Inositol 1,4,5-triphosphate Receptor Type 1 (IP3R1) 유전자의 다형성 및 형질 관련성 분석

  • 김남국 (농촌진흥청 국립축산과학원) ;
  • 김건석 (농촌진흥청 국립축산과학원) ;
  • 정유성 (농촌진흥청 국립축산과학원) ;
  • 문희주 (농촌진흥청 국립축산과학원) ;
  • 조용민 (농촌진흥청 국립축산과학원) ;
  • 윤두학 (농촌진흥청 국립축산과학원)
  • Received : 2009.07.17
  • Accepted : 2009.08.11
  • Published : 2009.08.01
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Abstract

Inositol 1,4,5-triphosphate receptor type 1 (IP3R1) is a $Ca^{2+}$ release channel that responds to the second messenger IP3 and that modulates diverse cellular functions such as contraction/excitation, secretion, gene expression and cellular growth. We discovered single nucleotide polymorphisms (SNPs) within IP3R1 gene and analyzed associations between gene polymorphisms and carcass traits in Korean cattle (Hanwoo) in order to develop novel DNA markers at genomic level. Three SNPs were detected at the position of g.1428617A>G, g.1418843C>T and g.1414377C>T with 24 unrelated Hanwoo samples by direct sequencing of the PCR products. We found that genotype of g.1414377C>T SNP was associated with live weight (P<0.05) and carcass weight (P<0.01) using the general linear model of SAS package. These results suggest that polymorphism of IP3R1 gene was associated with weight-related traits in Hanwoo.

본 연구는 한우 inositol 1,4,5-triphosphate receptor type1(IP3R1) 유전자를 대상으로 SNP를 발굴하고, 도체형질과의 관련성 분석을 위하여 수행하였다. PCR 및 염기서열 결정법을 통해 IP3R1 유전자내 3개의 SNP를 발굴하였고, 이중 intron 29에 위치하는 SNP의 경우 미 보고된 신규 SNP로 확인되었다. 발굴된 3개의 SNP를 대상으로 표현형 기록치를 보유한 후대검정우 583두에 대하여 유전자형 분석 및 관련성 분석을 수행하였다. 분석결과 3개의 SNP 중 g.1428617A>G SNP가 생시체중(P<0.05) 및 도체중(P<0.01)과 유의적인 상관관계가 있음을 확인할 수 있었다. 이러한 결과는 추후 한우 개량을 위한 유전자 마커로 활용이 가능할 것으로 판단된다.

Keywords

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