YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Anti-Obese Activity of HPJ Extract on High Fat Diet-Induced Obese Mice

고지방 식이로 유도된 비만 쥐에서 HPJ 추출물의 항비만 효과

  • Yuan, Hai-Dan (Pharmacology and Clinical Pharmacy Lab, College of Pharmacy, Kyung Hee University) ;
  • Quan, Hai-Yan (Pharmacology and Clinical Pharmacy Lab, College of Pharmacy, Kyung Hee University) ;
  • Zhang, Ya (Pharmacology and Clinical Pharmacy Lab, College of Pharmacy, Kyung Hee University) ;
  • Kim, Sung-Jib (Pharmacology and Clinical Pharmacy Lab, College of Pharmacy, Kyung Hee University) ;
  • Shin, Dae-Hee (Research Complex, Sungkyunkwan University) ;
  • Lim, Bang-Ho (Research Complex, Sungkyunkwan University) ;
  • Chung, Sung-Hyun (Pharmacology and Clinical Pharmacy Lab, College of Pharmacy, Kyung Hee University)
  • 원해단 (경희대학교 약학대학 약물학.임상약학교실) ;
  • 권해연 (경희대학교 약학대학 약물학.임상약학교실) ;
  • 장아 (경희대학교 약학대학 약물학.임상약학교실) ;
  • 김성집 (경희대학교 약학대학 약물학.임상약학교실) ;
  • 신대희 ((주)휴온스 중앙연구본부) ;
  • 임방호 ((주)휴온스 중앙연구본부) ;
  • 정성현 (경희대학교 약학대학 약물학.임상약학교실)
  • Received : 2009.06.17
  • Accepted : 2009.08.10
  • Published : 2009.10.31
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Abstract

In this study, we investigated the anti-obese activity of HPJ extract in C57BL/6J mice. The C57BL/6J mice were randomly divided into five groups: normal control group (Con), high fat diet control group (HFD), treatment groups with HPJ at 125 mg/kg (HPJ125), 250 mg/kg (HPJ250), or 500 mg/kg (HPJ500). To induce an obesity, mice were fed by a high fat diet for 6 weeks, and mice were administered with HPJ extract once a day for 8 weeks. At the end of treatment, we examined the effect of HPJ extract on body weight, plasma lipid, and lipogenic enzymes. HPJ extract was found to lower whole body and epididymal adipose tissue weights and lowered plasma levels of glucose, insulin, triglyceride (TG), total cholesterol (TC), non-esterified fatty acid (NEFA) and leptin, compared to those in HFD group. Histological analyses of the liver and fat tissues of mice treated with HPJ extract revealed significantly decreased number of lipid droplets and decreased size of adipocytes compared to the HFD group. In addition, HPJ extract preserved the morphological integrity of pancreatic islets. To elucidate an action mechanism of HPJ extract, Western blot and RT-PCR were performed using epididymal adipose tissues. HPJ extract up-regulated the levels of phosphorylated adenosine monophosphate-activated protein kinase (AMPK) and its substrate, acetyl-CoA carboxylasse (ACC). HPJ extract also attenuated lipogenic gene expressions of sterol regulatory element-binding protein $1{\alpha}$ (SREBP$1{\alpha}$), fatty acid synthase (FAS), sterol-CoA desaturase 1 (SCD1) and glycerol-3-phosphate acyltransferase (GPAT) in dose-dependent manners. In contrast, expressions of lipolytic genes such as peroxisome proliferator-activated receptor-$\alpha$ (PPAR-${\alpha}$) and CD36, and fatty acid $\beta$-oxidation gene, carnitine palmitoyltransferase-1 (CPT-1) were increased. These results suggest that HPJ extract ameliorates obesity through inhibiting synthesis of lipogenic enzymes as well as stimulating fatty acid oxidation resulting from activation of AMPK, and HPJ extract could be developed as a potential therapeutic agent for obese patients.

Keywords

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