Asian Journal of Turfgrass Science (아시안잔디학회지)

Turfgrass Society of Korea (한국잔디학회)
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Development of SCAR markers in Creeping bentgrass(Agrostis palustrics Huds.) cultivars

Creeping bentgrass(Agrostis palustrics Huds.) 품종별 SCAR markers 개발

  • Received : 2009.09.01
  • Accepted : 2009.11.22
  • Published : 2009.12.31
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Abstract

Creeping bentgrass (Agrostis palustrics Huds.) is cool season turfgrasse that is used for putting green in golf course. Creeping bentgrass cultivars are difficult to distinguish with the same species because of similar morphological characters and low level of genetic diversity. The SCAR markers using the specific DNA can be useful for differentiating between creeping bentgrass cultivars. Five RAPD primers were used for specific band detection among creeping bentgrass cultivars, penncross, penn A-4, crenshaw, L-93, CY-2, T-1. The pairs of SCAR primers for six cultivers were designed by the specific sequences of the bands that amplified by RAPD. Three of the six SCAR primers could not make the use as SCAR primers because the specific false bands were detected in all cultivars. The remaining pairs of SCAR primer, CY850F/R, T700F/R, L2900F/R, amplified the specific band at expected size for three cultivars, CY-2, T-1, L-93, respectively. The CY850F/R primer amplified a band of 850bp in CY-2 cultivar, the T700F/R primer amplified a band of 700bp in T-1 cultivar, and the L2900F/R primer amplified a band of 2.9kb in L-93 cultivar. In this study we developed the SCAR markers to identify and distinguish the inerseeded creeping bentgrass cultivars in a golf course green.

크리핑벤트그래스는 골프코스의 퍼팅그린에서 주로 사용하고 있는 한지형잔디 초종이다. 크리핑벤트그래스 품종들은 형태적인 특성이나 유전적인 다양성이 협소하여 품종간 구분이 매우 어렵다. 따라서 이들 품종간 유전적인 다양성이나 골프코스 그린의 인터씨딩율에 대한 조사를 위해 SCAR marker를 개발하고자 본 연구를 수행하였다. penncross, penn A-4, crenshaw, L-93, CY-2, T-1 공시품종들에 대한 SCAR marker 개발을 위해 5개 RAPD primer에 대한 품종 간 구분이 가능한 특이적 band를 선발하였다. 선발한 특이적 band의 염기서열을 분석하여 품종별 SCAR primer를 제작하였다. 각 품종의 SCAR primer별로 특이적 band가 나타나는지에 대한 여부를 검정한 결과, penncross, penn A-4, crenshaw 품종의 SCAR primer는 6개 공시품종에서 동일한 크기의 band가 나타나 SCAR marker로써 활용이 어려웠다. 하지만 CY-2의 CY850F/R primer는 850bp, T-1의 T700F/R primer는 700bp, L-93의 L2900F/R는 2.9kb에서 특이적 band가 나타나 3개 품종별 SCAR marker로서 활용이 가능하였다. 따라서 본 연구에서 개발한 3개 품종별 SCAR marker를 활용하여 크리핑벤트그래스의 품종별 유전적 다양 및 골프코스 그린의 인터씨딩율 조사에 활용이 가능할 것으로 기대된다.

Keywords

References

  1. 한국잔디연구소. http://www.ktri.or.kr
  2. Bonos, S.A., K.A. Plumley, and W.A. Meyer. 2002. Ploidy determination inAgrostis using flow cytometry and morphological traits. Crop Sci. 42:192-196 https://doi.org/10.2135/cropsci2002.0192
  3. Caceres, M.E., Pupilli F., Piano E., and S. Arcioni. 2000. RFLP markers are an effective tool for the identification of creeping bentgrass(Agrostis stolonifera L.)cultivars. Genetic Resources and Crop Evolution 47:455-459 https://doi.org/10.1023/A:1008751520046
  4. Cai, H., M. Inoue, N. Yuyama, and S. Nakayama. 2004. An AFLP-basedlinkage map of Zoysiagrass(Zoysia japonica). Plant Breeding 123:543-548 https://doi.org/10.1111/j.1439-0523.2004.01022.x
  5. Casler, M.D., and R.R, Duncan. 2003. Turfgrass biology, geneitcs, andbreeding. p 175. John Wiley & Sons, Inc., Hoboken, New Jersey
  6. Casler, M.D., Y. Rangel, and J.C., Stier, and G., Jung. 2003. PAPD marker diversity among creeping bentgrass clones. Crop Sci. 43:688-693
  7. Dieffenbach, C.W., T.M. Lowe, and G.S. Dveksler. 1993. General conceptsfor PCR primer design. Genome Res. 3:S30-S37 https://doi.org/10.1101/gr.3.3.S30
  8. Elizabeth, A.S., M.D., Casler, and G., Jung. 2003. Development of species-specific SCAR markers in bentgrass. Crop Sci. 43:345-349 https://doi.org/10.2135/cropsci2003.0345
  9. Hernandez, P., A. Martin, and G. Dorado. 1999. Development of SCARs by direct sequencing fo RAPD products: a practical tool for the introgression and marker-assisted selection of wheat. Mol. Breed. 5:245-253 https://doi.org/10.1023/A:1009637928471
  10. Hollman, A.B., J.C. Stier, M.D. Casler, G. Jung, and L.A. Brilman. 2005. Identification of putative velvet bentgrass clones using RAPD markers. Crop Sci. 45:923-930 https://doi.org/10.2135/cropsci2004.0297
  11. Huff. D.R., and A.J. Palazzo. 1998. Fine fescue species determination bylaser flow cytometry. Crop Sci. 38:445-450 https://doi.org/10.2135/cropsci1998.0011183X003800020029x
  12. Johnes, M.A., P.W. Skroch, J. Nienhuis, P.Hinrichsen, G. Bascur, and C. Munoz-Schick. 1997. Gene pool classification of common bean landraces from Chile based on RAPD and morphological data. Crop Sci. 37:605-613 https://doi.org/10.2135/cropsci1997.0011183X003700020049x
  13. Jones, T.W.A. 1983. Instability during storage of phosphogluco-isomerase isoenzymes from ryegrass(Lolium spp.). Physiol. Plant 58:136-140 https://doi.org/10.1111/j.1399-3054.1983.tb04156.x
  14. Murray, M G; Thompson, W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic acids research ,v.8 no.19 pp.4321-4325 https://doi.org/10.1093/nar/8.19.4321
  15. Paran, I, and R.W. Michelmore. 1993. Development of reliable RCR-based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Gen. 85:985-993
  16. Rossi, F.S. 1999. Interseeding bentgrasses into established turf. Golf Course Management August p:53-56
  17. Skroch, P., and J. Nienhuis. 1993. Impact of scoring error and reproducibility of RAPD data on RAPD based estimates of genetic distance. Theor. Appl. Gen.91:1086-1091
  18. Vergara, G.V., and S.S. Bughrara. 2004. Genetic differentiation of tetraploid creeing bentgrass and hexaploid redtop bentgrass genotypes by AFLP and their use in turfgrass breeding. Crop Sci. 44:884-890 https://doi.org/10.2135/cropsci2004.0884
  19. Warnke, S.E., D.S. Douches, and B.E. Branham. 1997. Relationships among creeping bentgrass cultivars based on isozyme polymorphism. Crop Sci. 37:203-207 https://doi.org/10.2135/cropsci1997.0011183X003700010034x
  20. Wilkinson, J.F., and J.B. Beard. 1972. Electrophoretic identification of Agrostis palustris and Poa pratensis cultivars. Crop Sci. 12:833-834 https://doi.org/10.2135/cropsci1972.0011183X001200060036x
  21. Yamamoto, I., and J.M. Duich. 1994. Electrophoretic identification of cross-pollinated bentgrass species and cultivars. Crop Sci. 34:792-798 https://doi.org/10.2135/cropsci1994.0011183X003400030034x
  22. Yaneshita, M., Nagasawa R., Engelke M.C., and Sasakuma T. 1997. Geneticvariation and interspecific hybridization among natural populations of zoysiagrasses detected by RFLP analyses of chloroplast and nuclear DNA. Genes Gener. Syst. 72:173-179 https://doi.org/10.1266/ggs.72.173