Asian Journal of Turfgrass Science (아시안잔디학회지)

Turfgrass Society of Korea (한국잔디학회)
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Antagonistic Effects of Pseudomonas spp. against Turfgrass Pathogenic Soil Fungi

잔디 주요 토양 병해에 대한 토양세균 Pseudomonas spp.의 길항 효과

  • Chang, Seog-Won (Bio-Regional Innovation Center Youngdong University) ;
  • Chang, Tae-Hyun (Department of Plant Resources, Division of Ecology and Environment System, College of Ecology and Environmental Sciences, KyungpookNational University) ;
  • Choi, Byung-Jin (Bio-Regional Innovation Center Youngdong University) ;
  • Song, Jung-Hee (Bio-Regional Innovation Center Youngdong University) ;
  • Park, Kyung-Sook (Bio-Regional Innovation Center Youngdong University) ;
  • Rho, Yong-Taek (Bio-Regional Innovation Center Youngdong University)
  • 장석원 (영동대학교바이오지역혁신센터) ;
  • 장태현 (경북대학교 자원식물학과) ;
  • 최병진 (영동대학교바이오지역혁신센터) ;
  • 송정희 (영동대학교바이오지역혁신센터) ;
  • 박경숙 (영동대학교바이오지역혁신센터) ;
  • 노용택 (영동대학교바이오지역혁신센터)
  • Received : 2009.11.10
  • Accepted : 2009.12.15
  • Published : 2009.12.31
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Abstract

Bacterial isolates collected from rhizosphere of turfgrass showed strong in vitro antagonistic activities against a number of turfgrass soilborne pathogens such as Rhizoctonia cerealis, R. solani AG-1(1B), Sclerotinia homoeocarpa and Typhula incarnata. In vivo study, four bacterial isolates selected have control values over 60% against one or more turfgrass pathogenic fungi. The antagonistic effects of the bacterial isolates varied depending on fungal species, host plant, and disease pressure, indicating that control effects of the antagonists could be variable depending on field conditions. They were classified as belonging to the genus Pseudomonas species, based on morphological and biochemical characteristics as well as 16S rRNA analysis. The four bacterial isolates are under a study for finding proper cultural conditions and determination formulation type.

본 연구에서는 골프장에서 문제가 되는 주요 병의 방제가능한 생물학적 제제를 개발하기 위하여 실시하였다. 강원도 소재 골프장의 잔디 근권토양으로부터 채취한 100여개의 미생물로부터 4개의 길항 미생물을 선발하였다. 배지를 이용한 저지원 실험에서 4개의 균주는 한국 골프장에서 문제가 되고 있는 옐로우패취(Rhizoctonia cerealis), 브래운패취(R. solani AG-1(1B)), 동전마름병(Sclerotinia homoeocarpa), 그리고 설부갈색소립균핵병원균 (Typhula incarnata)에 대하여 효과적이었다. 옐로우패취, 브래운패취, 동전마름병을 대상으로 온실실험 결과, 4개의 균주는 병의 종류, 기주 식물체 및 병 발생 정도에 따라 효과가 달랐지만 한개 혹은 그 이상의 병에 대해 60% 이상을 방제가를 기록하였다. 4개의 균주는 생화학적 특성과 16S rRNA 분석을 통해 Pseudomonas spp.로 밝혀졌다. 현재 4개의 균주는 적정 배양조건과 제제화를 위한 연구 중에 있다.

Keywords

References

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