한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제17권3호
  • /
  • Pages.198-203
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  • 2009
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

대청에서 AFLP를 이용한 종자순도검사와 평가

Seed Purity Test and Evaluation in Isatis tinctoria var. yezoensis (Ohwi) Ohwi Using AFLP Markers

  • 최주수 (동의대학교 분자생물학과) ;
  • 허만규 (동의대학교 분자생물학과) ;
  • 성정숙 (농촌진흥청 농업유전자원센터)
  • Choi, Joo-Soo (Department of Molecular Biology, Dong-eui University, Department of Biology, Huck Institutes of the Life Sciences, Pennsylvania State University) ;
  • Huh, Man-Kyu (Department of Molecular Biology, Dong-eui University, Department of Biology, Huck Institutes of the Life Sciences, Pennsylvania State University) ;
  • Sung, Jung-Sook (National Agrobiodiversity center, RDA)
  • 발행 : 2009.06.30
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초록

Isatis tinctoria var. yezoensis (Ohwi) Ohwi (Cruciferae) is one of major natural dyeing crops in the world and also have used as a medicinal plant in Korea. We evaluated seed purity in $F_1$-hybrid accessions using amplified fragment length polymorphism (AFLP) markers. One hundred sixty seeds from the male and female harvests were subsequently screened for seed purity with ten primers. The 13 accession-specific bands and many variable AFLP bands scored for accessions. Especially, E-AAC/M-CAA and E-AAG/M-CAT were presented clear hybrid bands for $F_1$ hybrids. $F_1$ hybrids maintained higher average level of genetic diversity compared with their correspondent parents. Self-inbred seeds from the female and male harvests were revealed 8.0% and 5.0%, respectively. The AFLP may lead to a better insight in to the hybrid seed purity test in I. tinctoria var. yezoensis.

키워드

참고문헌

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