Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Plant Regeneration through Organogenesis from Callus of Camptotheca acuminata Decaisne

희수나무 캘러스로부터 기관분화에 의한 식물체 재분화

  • Bae, Dae-Ho (Department of Biology and Institute of Plant Resources, Chonnam National Univ.) ;
  • Park, Whoa-Shig (Forest Resources Research Institute) ;
  • Hwang, Sung-Jin (Department of Biology and Institute of Plant Resources, Chonnam National Univ.) ;
  • Hwang, Baik (Department of Biology and Institute of Plant Resources, Chonnam National Univ.)
  • Published : 2009.06.30
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Abstract

Camptotheca acuminata, a native of South China is a well known natural source of monoterpene-indole alkaloid camptothecin(CPT), one of the most promising anti-tumoural compounds. This study was conducted to optimize plant growth regulators and culture conditions on plantlets regeneration through organogenesis from callus of Camptotheca acuminta. Callus were induced from various explants of in vitro germinated plantlets of C. acuminta using WPM medium containing 0.2 ㎎/L 2,4-D. Hypocotyl segments were exhibited higher embryogenic callus than the other explants. Shoot buds formation from embryogenic callus was affected by plant growth regulators, pre-treated dark condition and liquid culture. Organogenesis was optimal in WPM liquid medium containing 0.5 ㎎/L BA. The dark pre-treatment for 2 weeks before the solid culture was effective for organogenesis. The regenerated shoots were rooted in WPM medium with 0.2 ㎎/L NAA and successfully acclimated in green-house conditions.

Keywords

References

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